Development of evaluation method of soil microorganisms evenness
| Project/Area Number |
23380196
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
SHINANO Takuro 独立行政法人農業・食品産業技術総合研究機構, 東北農業研究センター農業放射線研究センター, センター長 (20235542)
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| Co-Investigator(Kenkyū-buntansha) |
OKAZAKI Keiki 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター・生産環境研究領域, 主任研究員 (40414750)
SEKIGUCHI Hiroyuki 独立行政法人農業・食品産業技術総合研究機構, 近畿中国四国農業研究センター・水田作研究領域, 主任研究員 (50466009)
IWATA Yukiyoshi 独立行政法人農業・食品産業技術総合研究機構, 農村工学研究所・農地基盤工学研究領域, 主任研究員 (70370591)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
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| Keywords | 土壌微生物 / 一酸化二窒素 / メタゲノム / メタトランスクリプトーム / 凍結融解 / 多様性指数 / 次世代シーケンシング / 病害土壌 / 根圏土壌 / 土壌RNA |
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| Research Abstract |
We focus on nitrous oxide emission during freeze and thaw process in the field, and to investigate the diversity and evenness of the soil microorganism, metagenomic and metatranscriptomic approaches were applied on DNA and mRNA levels. The results indicate that during the bio-geochemical process, it is rather evenness play an important role than diversity of species number, indicating that few number of species seems to be increased with the process. We have applied the Functional Single Cell separation technique and obtain several isolates which seem to grow and lead the process dominantly under the cold condition.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Effect of phosphorus levels on the protein profiles of secreted protein and root surface protein of rice2013
Author(s)
Shinano, T., Yoshimura, T., Watanabe, T., Unno, Y., Osaki, M., Nanjo, Y. and Komatsu, S.
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Journal Title
Journal of Proteome Research
Volume: 12(11)
Issue: 11
Pages: 4748-4756
DOI
Related Report
Peer Reviewed
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[Presentation] Evaluation of potential risks of soils for Fusarium wilt of spinach using nitrate-nonutilizing mutants of the pathogen, Fusarium oxysporum f. sp. spinaciae.2013
Author(s)
Sekiguchi, H., Takehara, T., Tomioka, K., Nomiyama, K., Osaki, H., Miyagawa, H. and Shinano, T.
Organizer
The 10th International Congress of Plant Pathology
Place of Presentation
Beijing, China
Related Report
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[Presentation] Decreasing soil frost depth and its influence on the soil water and nitrate movements in Tokachi, Hokkaido, Japan2012
Author(s)
Iwata, Y., Hirota, T., Hayashi, M., Yazaki, T., Nemoto, M., Ohkubo, S., Yanai, Y., Suzuki, S
Organizer
Plant and Microbe Adaptations to Cold
Place of Presentation
札幌, 日本
Related Report
Invited
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[Presentation] 根の世界2011
Author(s)
信濃卓郎
Organizer
サッポロ農学校2011
Place of Presentation
北海道大学農学部(札幌)
Year and Date
2011-09-15
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[Book] Plant and microbe adaptations to cold in a changing world, p41-52, Possible change of water and nitrate cycles associated with the frost-depth decrease under climate change2013
Author(s)
Iwata, Y., Hirota, T., Hayashi, M, Arima, J., Suzuki, S., Nemoto, M
Total Pages
352
Publisher
Springer
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[Book] Possible change of water and nitrate cycles associated with the frost-depth decrease under climate change2013
Author(s)
Iwata, Y., Hirota, T., Hayashi, M, Arima, J., Suzuki, S., Nemoto, M.
Total Pages
352
Publisher
Springer
Related Report
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