Escherichia coli host engineering for non-biased expression of metagenomic library
Project/Area Number |
23380197
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Boundary agriculture
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Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
MIYAZAKI Kentaro 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (60344123)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
|
Keywords | メタゲノム / バイオテクノロジー / 大腸菌 / ゲノム育種 / リボソーム工学 / 16S rRNA / 翻訳 / リボソーム / 宿主 / 機能改変 / 遺伝子発現 / 異宿主発現 / レポーターアッセイ / 翻訳開始因子 / 発現バイアス / 進化工学 / 開始コドン |
Research Abstract |
We have applied translational engineering approach to solve the problem in heterologous expression problem in Escherichia coli. Initiation factor 3 as well as the ribosome was engineered to alter the translational profile and variants that showed enhanced expression of genes having non-AUG initiation codon were identified. Also, mutant ribosome that have 16S rRNA from non-E. coli bacteria showed altered, or enhanced expression of reporter gfp genes, that were not efficiently expressed in wild type E. coli.
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Report
(4 results)
Research Products
(120 results)
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[Journal Article] Meeting Report : 1st International Functional Metagenomics Workshop2013
Author(s)
Engel K, Ashby D, Brady SF, Cowan DA, Doemer J, Edwards EA, Fiebig K, Martens EC, McCormac D, Mead DA, Miyazaki K, Moreno-Hagelsieb G, O'Gara F, Reid A, Rose DR, Simonet P, Sjöling S, Smalla K, Streit WR, Tedman-Jones J, Valla S, Wellington EMH, Wu C-C, Liles MR, Neufeld JD, Sessitsch A, Charles TC
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Journal Title
St. Jacobs, Ontario, Canada. Stand Genomic Sci
Volume: 8
Pages: 106-111
Related Report
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[Journal Article] Meeting Report: 1st International Functional Metagenomics Workshop May 7–8, 2012, St. Jacobs, Ontario, Canada2013
Author(s)
Engel K, Ashby D, Brady SF, Cowan DA, Doemer J, Edwards EA, Fiebig K, Martens EC, McCormac D, Mead DA, Miyazaki K, Moreno-Hagelsieb G, O'Gara F, Reid A, Rose DR, Simonet P, Sjöling S, Smalla K, Streit WR, Tedman-Jones J, Valla S, Wellington EMH, Wu C-C, Liles MR, Neufeld JD, Sessitsch A, Charles TC
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Journal Title
Standards in Genomic Sciences
Volume: 8
Issue: 1
Pages: 106-111
DOI
Related Report
Peer Reviewed
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