| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Research Abstract |
We have applied translational engineering approach to solve the problem in heterologous expression problem in Escherichia coli. Initiation factor 3 as well as the ribosome was engineered to alter the translational profile and variants that showed enhanced expression of genes having non-AUG initiation codon were identified. Also, mutant ribosome that have 16S rRNA from non-E. coli bacteria showed altered, or enhanced expression of reporter gfp genes, that were not efficiently expressed in wild type E. coli.
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