| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Outline of Final Research Achievements |
We previously found that a plasmid bearing mammalian replication initiation region (IR) and a nuclear matrix attachment region (MAR) was amplified spontaneously in animal cells and generate extrachromosomal DMs or chromosomal HSR. This study aimed to develop this method to a novel cell technology. Consequently, 1) we identified minimal required sequence for gene amplification, and clarified the relationship to DNA replication. 2) We clarified the mechanism of gene amplification. 3) We isolated a human genome sequence that enhance the gene expression from tandemly repeated sequence.4) We showed that the pulverization of chromosome de novo generates DMs that had amplified oncogenes. 5) We obtained the understanding about the intracellular maintenance and extracellular elimination of DMs.
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