Biosynthetic pathway and physiological role of pyruvylated galactose-containing oligosaccharides in fission yeast.
| Project/Area Number |
23380204
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
TAKEGAWA Kaoru 九州大学, (連合)農学研究科(研究院), 教授 (50197282)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | 分裂酵母 / 糖鎖 / ガラクトース / ピルビン酸 / 糖タンパク質 / 非性的凝集 / 凝集素 |
|---|
| Research Abstract |
In the fission yeast Schizosaccharomyces pombe, galactose residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases in the lumen of the Golgi apparatus. Cell surface glycoproteins play a key role in flocculation and filamentous invasive growth in yeasts. We identified fission yeast gsf2+, encoding a flocculin that binds to galactose residues located on cell surface glycoconjugates. Flocculation and invasive growth of S. pombe is tightly controlled by gsf2+ expression. MADS-box transcription factor Mbx2 regulates nonsexual flocculation by inducing gsf2+ expression. S. pombe appears to have a unique galactose-specific recognition system in which Gsf2p/flocculin plays an essential role in mediating cell-cell interactions. Furthermore, we characterized a putative S. pombe pyruvyltransferase, Pvg1p, and the recombinant Pvg1p transferred pyruvyl residues from phosphoenolpyruvate specifically to beta-linked galactose.
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Report
(4 results)
Research Products
(117 results)
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[Journal Article] gfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and A. fumigatus2013
Author(s)
Komachi Y, Hatakeyama S, Motomatsu H, Futagami T, Kinzjakina K, Sorbado P, Ekino K, Takegawa K, Goto M, Nomura Y, and Oka T
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Journal Title
Mol Microbiol
Volume: 90(5)
Pages: 1054-1073
Related Report
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[Journal Article] gfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and A. fumigatus.2013
Author(s)
Komachi Y, Hatakeyama S, Motomatsu H, Futagami T, Kinzjakina K, Sorbado P, Ekino K, Takegawa K, Goto M, Nomura Y, and Oka T
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Journal Title
Molecular Microbiology
Volume: 90
Issue: 5
Pages: 1054-1073
DOI
Related Report
Peer Reviewed
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[Presentation] 酵母の糖鎖認識システム2011
Author(s)
竹川薫
Organizer
第2回糖鎖科学中部拠点プログラム講演会
Place of Presentation
名古屋大学(名古屋市)(招待講演)
Year and Date
2011-09-24
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