Development of nano-sized DDS for nucleic acid drugs based on polypod-like structured nucleic acids
| Project/Area Number |
23390010
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
NISHIKAWA Makiya 京都大学, 薬学研究科(研究院), 准教授 (40273437)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Yoshinobu 京都大学, 大学院・薬学研究科, 教授 (30171432)
TAKAHASHI Yuki 京都大学, 大学院・薬学研究科, 助教 (00547870)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
|---|
| Keywords | 核酸 / ナノ粒子 / DDS / CpG / siRNA |
|---|
| Research Abstract |
To increase the therapeutic potency of nucleic acid drugs, we r tried to develop polypod-like structured nucleic acid, or polypodna, and dendritic nano-sized DDS by connecting them. We found that polypodna with 3 to 8 pods can be developed and these DNA nanostructures are efficiently taken up by immune cells depending on pod number. Furthermore, we succeeded in constructing DNA dendrimer by connecting polypodnas through their adhesive terminal ends. Cytokine release by immunostimulatory CpG DNA has been greatly increased by the development of such DNA nanostructures. Thus, we have shown that this approach can be useful for increasing the potency of nucleic acid drugs.
|
Report
(4 results)
Research Products
(67 results)
-
-
[Journal Article] Injectable, self-geling, biodegradable, and immunomodulatory DNA hydrogel for antigell dellvery2014
Author(s)
Makiya Nishikawa, Kohei Ogawa, Yaka Umeki, KohtaMohri, Yohji Kawashaki, Hiroshi Watanabe, Natsuki Takahashi, Eri Kusuki, Rei Takahashi, Yuki Takahashi, Yoshinobu Takakura
-
Journal Title
Journal of Controlled Release
Volume: 180
Pages: 25-32
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Design and development of nanosized DNA assemblies in polypod-like structures as efficient vehicles for immunostimulatory CpG motifs to immune cells2012
Author(s)
Mohri K, Nishikawa M, Takahashi N, Shiomi T, Matsuoka N, Ogawa K, Endo M, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y
-
Journal Title
ACS Nano
Volume: 6
Issue: 7
Pages: 5931-5940
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 多足型核酸構造体-ポリポドナ-の設計と構造・機能解析2012
Author(s)
西川元也, 毛利浩太, 高橋夏樹, 楠木絵理, 塩見朋紀, 小川耕平, 梅木佑夏, 遠藤政幸, 杉山 弘, 渡辺 宏, 高橋有己, 高倉喜信
Organizer
アンチセンス・遺伝子・デリバリーシンポジウム2012
Place of Presentation
仙台
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 酵素反応を必要としないDNAハイドロゲルの開発2011
Author(s)
毛利浩太, 西川元也, 小川耕平, 高橋夏樹, 楠木絵里, 高橋有己, 高倉喜信
Organizer
第21アンチセンスシンポジウム+第11回遺伝子・デリバリー研究会シンポジウム合同シンポジウム
Place of Presentation
大阪大学コンベンションセンター(大阪府)
Year and Date
2011-09-02
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
