Molecular mechanism for roles of chondroitin sulfate in tumor metastasis

• Project/Area Number: 23390016
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: SUGAHARA Kazuyuki 北海道大学, 先端生命科学研究科(研究院), 学術研究員 (60154449)
• Co-Investigator(Kenkyū-buntansha): YAMADA Shuhei 北海道大学, 大学院・先端生命科学研究院, 准教授 (70240017)
• Co-Investigator(Renkei-kenkyūsha): MIZUMOTO Shuji 名城大学, 薬学部, 助教 (40443973)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 癌 / 転移 / コンドロイチン硫酸 / ヘパラン硫酸 / RAGE / ルイス肺癌細胞 / グリコサミノグリカン / プロテオミクス / プロテオグリカン / オリゴ糖 / デルマタン硫酸

Research Abstract

Previously, we have demonstrated that Lewis lung carcinoma (LLC)-derived tumor cell line with high metastatic potential shows a higher proportion of E-disaccharide units, GlcUA-GalNAc(4, 6-O-disulfate), in CS chains, which are recognized by phage display antibody. Interestingly, the antibody strongly inhibited the pulmonary metastasis. In this research, to elucidate the molecular mechanism for tumor metastasis involved in the CS chains. The receptor molecule for CS chains containing E-disaccharides, Receptor for Advanced Glycation End-products (RAGE), was identified by proteomics approach. Furthermore, the colonization of the lungs by LLC cells was effectively inhibited by an anti-RAGE antibody. These results provide the clear evidence that RAGE is at least one of the critical receptors for CS chains expressed at the tumor cell surface and involved in experimental lung metastasis, and that CS/HS and RAGE are potential molecular targets in the treatment of pulmonary metastasis.

Research Products

• [Journal Article] Glycosaminoglycans are functional ligands for Receptor for Advanced Glycation End-products (RAGE) in tumors (2013)
  • Author(s): Shuji Mizumoto, Kazuyuki Sugahara
  • Journal Title: FEBS Journal Volume: 280(10) Pages: 2462-2470
  • Peer Reviewed
• [Journal Article] Expression of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase involved in chondroitin sulfate synthesis is responsible for pulmonary metastasis (2013)
  • Author(s): Shuji Mizumoto, Moto Watanabe, Shuhei Yamada, Kazuyuki Sugahara
  • Journal Title: BioMed Research International Volume: 2013 Pages: 656319-656319
  • NAID: 120005906914
  • Peer Reviewed
• [Journal Article] Glycosaminoglycans are functional ligands for Receptor for Advanced Glycation End-products (RAGE) in tumors (2013)
  • Author(s): Mizumoto, S., and Sugahara, K.
  • Journal Title: FEBS Journal Volume: 280 Issue: 10 Pages: 2462-2470
  • DOI: 10.1111/febs.12156
  • Peer Reviewed
• [Journal Article] Expression of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase involved in chondroitin sulfate synthesis is responsible for pulmonary metastasis (2013)
  • Author(s): Mizumoto, S., Watanabe, M., Yamada, S., and Sugahara, K.
  • Journal Title: BioMed Research International Volume: 2013 Pages: 656319-656319
  • DOI: 10.1155/2013/656319
  • NAID: 120005906914
  • Peer Reviewed
• [Journal Article] Specific interaction of the envelope glycoproteins E1 and E2 with liver heparan sulfate involved in the tissue tropismatic infection by hepatitis C virus (2012)
  • Author(s): Fumi Kobayashi, Shuhei Yamada, Shuhei Taguwa, Chikako Kataoka, Satomi Naito, Yoshiki Hama, Hideki Tani, Yoshiharu Matsuura, Kazuyuki Sugahara
  • Journal Title: Glycoconjugate Journal Volume: 29(4) Pages: 211-220
  • Peer Reviewed
• [Journal Article] Receptor for Advanced Glycation End Products (RAGE) Functions as a Receptor for Specific Sulfated Glycosaminoglycans, and Anti-RAGE Antibody or The Sulfated Glycosaminoglycans Delivered in vivo Inhibit Pulmonary Metastasis of Tumor Cells (2012)
  • Author(s): Shuji Mizumoto, Jun Takahashi, Kazuyuki Sugahara
  • Journal Title: Journal of Biological Chemistry Volume: 287(23) Pages: 18985-18994
  • Peer Reviewed
• [Journal Article] Receptor for Advanced Glycation End Products (RAGE) Functions as a Receptor for Specific Sulfated Glycosaminoglycans, and Anti-RAGE Antibody or The Sulfated Glycosaminoglycans Delivered in vivo Inhibit Pulmonary Metastasis of Tumor Cells (2012)
  • Author(s): Mizumoto, S., Takahashi, J., and Sugahara, K.
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 23 Pages: 18985-18994
  • DOI: 10.1074/jbc.m111.313437
  • Peer Reviewed
• [Journal Article] Specific interaction of the envelope glycoproteins E1 and E2 with liver heparan sulfate involved in the tissue tropismatic infection by hepatitis C virus (2012)
  • Author(s): Kobayashi, F., Yamada, S., Taguwa, S., Naito, S., Tani, H., Matsuura, Y., and Sugahara, K.
  • Journal Title: Glycoconjugate Journal Volume: 29 Issue: 4 Pages: 211-220
  • DOI: 10.1007/s10719-012-9388-z
  • Peer Reviewed
• [Journal Article] ISCSM2011 Chondroitin sulfate E-type structure at tumor cell surface is involved in experimental metastasis (2012)
  • Author(s): Sugahara, K., and Mizumoto, S.
  • Journal Title: Advances in Experimental Medicine and Biology Volume: 749 Pages: 33-45
  • DOI: 10.1007/978-1-4614-3381-1_3
  • ISBN: 9781461433804, 9781461433811
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate E-type structure at tumor cell surface is involved in experimental metastasis (2012)
  • Author(s): Kazuyuki SUGAHRA, Shuji MIZUMOTO
  • Journal Title: Advances in Experimental Medicine and Biology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Bone ana skin disorders caused by a disturbance in the bioynthesis of chondroitin sulfate and dermatan sulfate (2012)
  • Author(s): Mizumoto, S., Sugahara, K.
  • Journal Title: Extracellular matrix : Pathobiology and signaling Volume: (in press)
  • Peer Reviewed
• [Journal Article] Receptor for Advanced Glycation End Products (RAGE) Functions as a Receptor for Specific Sulfated Glycosaminoglycans, and Anti-RAGE Antibody or The Sulfated Glycosaminoglycans Delivered in vivo Inhibit Pulmonary Metastasis of Tumor Cells (2012)
  • Author(s): Shuji MIZUMOTO, Jun TAKAHASHI, Kazuyuki SUGAHARA
  • Journal Title: Journal of Biological Chemistry Volume: (in press)
  • Peer Reviewed
• [Journal Article] Involvement of HNK-1 Sulfotransferase in the Biosynthesis of Chondroitin Sulfate and the GlcUA(3-O-sulfate)-Gal-Gal-Xyl Tetrasaccharide Found in α-Thrombomodulin from Human Urine (2011)
  • Author(s): Taishi Hashiguchi, Shuji Mizumoto, Yuko Nishimura, Jun-ichi Tamura, Shuhei Yamada, Kazuyuki Sugahara
  • Journal Title: Journal of Biological Chemistry Volume: 286(38) Pages: 33003-33011
  • Peer Reviewed
• [Journal Article] Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus (2011)
  • Author(s): Jiancheng Chen, Shuhei Yamada, Yoshiki Hama, Ajaya Kumar Shetty, Takanari Kobayashi, Hiroshi Oda, Kosuke Seiki, Eunmi Kim, Takashi Kimura, Naonori Takahashid, Kazuya Hidari, Takashi Suzuki, Yasuo Suzuki, Kazuyuki Sugahara
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 412(1) Pages: 136-142
  • Peer Reviewed
• [Journal Article] Faulty initiation of proteoglycan synthesis causes cardiac and joint defects (2011)
  • Author(s): Sevjidmaa Baasanjav, Lihadh Al-Gazali, Taishi Hashiguchi, Shuji Mizumoto, Bjoern Fischer, Denise Horn, Dominik Seelow, Bassam R. Ali, Samir A.A. Aziz, Ruth Langer, Ahmed A.H. Saleh, Christian Becker, Gudrun Nürnberg, Vincent Cantagrel, Joseph G. Gleeson, Delphine Gomez, Jean-Baptiste Michel, Sigmar Stricker, Tom H. Lindner, Peter Nürnberg, Kazuyuki Sugahara, Stefan Mundlos, Katrin Hoffmann
  • Journal Title: American Journal of Human Genetics Volume: 89(1) Pages: 15-27
  • Peer Reviewed
• [Journal Article] Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection (2011)
  • Author(s): Eunmi Kim, Megumi Okumura, Hirofumi Sawa, Tadaaki Miyazaki, Daisuke Fujikura, Shuhei Yamada, Kazuyuki Sugahara, Michihito Sasaki, Takashi Kimura
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 409(4) Pages: 717-722
  • Peer Reviewed
• [Journal Article] Dermatan sulfate epimerase 2 is the predominant isozyme in the formation of the chondroitin sulfate/dermatan sulfate hybrid structure in postnatal developing mouse brain (2011)
  • Author(s): Chizuru Akatsu, Shuji Mizumoto, Tomoyuki Kaneiwa, Marco Maccarana, Anders Malmström, Shuhei Yamada, Kazuyuki Sugahara
  • Journal Title: Glycobiology Volume: 21(5) Pages: 565-574
  • Peer Reviewed
• [Journal Article] Evolution of Glycosaminoglycans : Comparative Biochemical Study (2011)
  • Author(s): Shuhei Yamada, Kazuyuki Sugahara, Suat Özbek
  • Journal Title: Communicative & Integrative Biology Volume: 4 Pages: 150-158
  • Peer Reviewed
• [Journal Article] Demonstration of the hepatocyte growth factor signaling pathway in the in vitro neuritogenic activity of chondroitin sulfate from ray fish cartilage (2011)
  • Author(s): Taishi Hashiguchi, Takanari Kobayashi, Duriya Fongmoon, Ajaya Kumar Shetty, Shuji Mizumoto, Nobuyuki Miyamoto, Toshikazu Nakamura, Shuhei Yamada, Kazuyuki Sugahara
  • Journal Title: Biochimica et Biophysica Acta (General Subjects) Volume: 1810(4) Pages: 406-413
  • NAID: 120002934270
  • Peer Reviewed
• [Journal Article] Demonstration of the hepatocyte growth factor signaling pathway in the in vitro neuritogenic activity of chondroitin sulfate from ray fish cartilage (2011)
  • Author(s): Taishi Hashiguchi
  • Journal Title: Bionchim.Biophys.Acta Volume: 1810 Issue: 4 Pages: 406-413
  • DOI: 10.1016/j.bbagen.2011.01.001
  • Peer Reviewed
• [Journal Article] Dermatan sulfate epimerase 2 is the predominant isozyme in the formation of the chondroitin sulfate/dermatan sulfate hybrid structure in postnatal developing mouse brain (2011)
  • Author(s): Akatsu, C., Mizumoto, S., Kaneiwa, T., Maccarana, M., Malmstrom,. A., Yamada, S., Sugahara, K.
  • Journal Title: Glycobiology Volume: 21 Issue: 5 Pages: 565-574
  • DOI: 10.1093/glycob/cwq208
  • Peer Reviewed
• [Journal Article] Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection (2011)
  • Author(s): Kim, E., Okumura, M., Sawa, H., Miyazaki, T., Fujikura, D., Yamada, S., Sugahara, K., Sasaki, M., Kimura, T.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 409 Issue: 4 Pages: 717-722
  • DOI: 10.1016/j.bbrc.2011.05.072
  • Peer Reviewed
• [Journal Article] Faulty initiation of proteoglycan synthesis causes cardiac and joint defects (2011)
  • Author(s): Baasanjav, S., Al-Gazali, L., Hashiguchi, T., Mizumoto, S., Fischer, B., Horn, D., Seelow, D., Ali, B.R., Aziz, S.A.A., Langer, R., Saleh,A.A.H., Becker, C., Nunberg,G., Cantagrel, V., Gleeson, J.G., Gomez, D., Michel, J-.B., Stricker, S., Lindner, T.H., Nunberg, P., Sugahara, K., Mundlos, S., Hoffmann, K.
  • Journal Title: American Journal of Human Genetics Volume: 89 Issue: 1 Pages: 15-27
  • DOI: 10.1016/j.ajhg.2011.05.021
  • Peer Reviewed
• [Journal Article] Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus (2011)
  • Author(s): Jiancheng Chen
  • Journal Title: Biochem.Biophys.Res.Commun Volume: 412 Issue: 1 Pages: 136-142
  • DOI: 10.1016/j.bbrc.2011.07.059
  • Peer Reviewed
• [Journal Article] Involvement of HNK-1 sulfotransferase in the biosynthesis of chondro itin sulfate and the GlcA(3-O-sulfate)-Gal-Gal-Xyl tetrasaccharide found in α-thrombomodulin from human urine (2011)
  • Author(s): Taishi Hashiguchi
  • Journal Title: J.Biol.Chem Volume: 286 Issue: 38 Pages: 33003-33011
  • DOI: 10.1074/jbc.m111.279174
  • Peer Reviewed
• [Presentation] Mechanism of glycosaminoglycan -mediated tumor metastasis (2014)
  • Author(s): Kazuyuki Sugahara, Shuji Mizumoto
  • Organizer: The 27th International Carbohydrate Symposium
  • Place of Presentation: Indian Institute of Science (India)
  • Year and Date: 2014-01-15
  • Invited
• [Presentation] Wobble chondroitin sulfate oligosaccharide motifs and mass production of oligosaccharides (2013)
  • Author(s): Kazuyuki Sugahara
  • Organizer: The 21st Symposium on Glycosaminoglycans
  • Place of Presentation: Villa Vigoni (Italy)
  • Year and Date: 2013-09-19
  • Invited
• [Presentation] Receptor for Advanced Glycation End-products (RAGE) Functions as Receptor for Sulfated Glycosaminoglycans (GAGs) : Anti-RAGE Antibody or Sulfated GAGs Inhibit Lung Metastasis of Tumor Cells (2013)
  • Author(s): Kazuyuki Sugahara, Jun Takahashi, Shuji Mizumoto
  • Organizer: 22nd International Symposium on Glycoconjugates
  • Place of Presentation: Dalian Institute of Chemical Phisics, Chinese Academy of Sciences (China)
  • Year and Date: 2013-06-28
• [Presentation] Development of novel monoclonal antibodies against chondroitin sulfate, and their application to glycobiology and glycopathology (2013)
  • Author(s): Peraphan Pothacharoen, Duriya Fongmoon, Shuji Mizumoto, Kazuyuki Sugahara, Prachya Kongtawlert
  • Organizer: The 5th annual conference for Asian Communications of Glycobiology and Glycotechnology
  • Place of Presentation: Khon Kaen University (Thailand.)
• [Presentation] Novel Class of Tetrahydropyridine-fused Pyrazoles, Isoxazolines, and HS-Mimetic Small Molecules as Anti-cancer Agents (2013)
  • Author(s): Rangappa KS, Basappa, Mantelingu K, Sadashiva MP, Feng Li, Gautam Sethi, Kazuyuki Sugahara
  • Organizer: The 14th tetrahedron symposium
  • Place of Presentation: Hilton Vienna (Austria)
• [Presentation] Mass preparation of oligosaccharides with intestinal absorption capacity by hydrolysis of chondroitin sulfate polysaccharides with subcritical water microreaction system (2013)
  • Author(s): Kazuyuki Sugahara, Shuhei Yamada, Shuji Mizumoto, Keiichiro Matsushima, Haruo Ura, and Nobuyuki Miyamoto
  • Organizer: The 13th International Conference on Functional and Medical Foods with Bioactive Compounds : Science and Practical Applications/The First International Symposium of Academic Society for Functional Foods and Bioactive Compounds
  • Place of Presentation: The Kyoto Prefectural University (Kyoto)
• [Presentation] Novel Class of Tetrahydropyridine-fused Pyrazoles, Isoxazolines, and HS-Mimetic Small Molecules as Anti-cancer Agents (2013)
  • Author(s): Rangappa KS, Basappa, Mantelingu K, Sadashiva MP, Feng Li, Gautam Sethi, Kazuyuki Sugahara
  • Organizer: The 14th tetrahedron symposium
  • Place of Presentation: Hilton Vienna(オーストリア)
• [Presentation] Development of novel monoclonal antibodies against chondroitin sulfate, and their application to glycobiology and glycopathology (2013)
  • Author(s): Peraphan Pothacharoen, Duriya Fongmoon, Shuji Mizumoto, Kazuyuki Sugahara,Prachya Kongtawlert
  • Organizer: The 5th annual conference for Asian Communications of Glycobiology and Glycotechnology
  • Place of Presentation: Khon Kaen University (タイ国)
• [Presentation] Receptor for Advanced Glycation End-products (RAGE) functions as a receptor for glycosaminoglycans involved in pulmonary metastasis (2012)
  • Author(s): Shuji Mizumoto, Jun Takahashi, Kazuyuki Sugahara
  • Organizer: The 10th International Symposium for Future Drug Discovery and Medical Care
  • Place of Presentation: Hokkaido Univ. (Sapporo)
  • Year and Date: 2012-10-03
• [Presentation] GAG signals involved in pulmonary metastasis of tumor cells (2012)
  • Author(s): Kazuyuki Sugahara
  • Organizer: The 20th Symposium on glycosaminoglycans
  • Place of Presentation: Villa Vigoni (Italy)
  • Year and Date: 2012-09-20
• [Presentation] RAGEは癌細胞の肺転移に関わるコンドロイチン硫酸の受容体として機能する (2012)
  • Author(s): 水本秀二, 高橋潤, 菅原一幸
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島市民ホール(鹿児島市)
  • Year and Date: 2012-09-20
• [Presentation] 糖鎖生物学による癌の肺転移の分子メカニズムの解明 抗癌剤開発に向けての洞察 (2012)
  • Author(s): 菅原一幸
  • Organizer: 第16回未来創薬・医療イノベーションセミナー(国際会議)
  • Place of Presentation: 北海道大学 (札幌市)
  • Year and Date: 2012-07-31
  • Invited
• [Presentation] がんの肺転移に関わるグリコサミノグリカン受容体(RAGE)の同定 (2012)
  • Author(s): 高橋潤, 水本秀二, 菅原一幸
  • Organizer: 第49回日本生化学会北海道支部例会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-07-20
• [Presentation] Characterization of a receptor for glycosaminoglycans that mediate experimental lung metastasis (2012)
  • Author(s): Kazuyuki Sugahara
  • Organizer: The 15th Gordon Research Conference on Proteoglycans
  • Place of Presentation: Proctor Academy (USA)
  • Year and Date: 2012-07-10
  • Invited
• [Presentation] Receptor for Advanced Glycation End Products (RAGE)は癌細胞の肺転移に関わるコンドロイチン硫酸の受容体として機能する (2012)
  • Author(s): 水本秀二, 高橋潤, 菅原一幸
  • Organizer: 日本薬学会北海道支部第138回例会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2012-06-16
• [Presentation] ユニークな構造と機能をもつエビ頭部に由来するヘパラン硫酸 (2012)
  • Author(s): 羽磨紀城, 陳建成, Ajaya Kumar Shetty, 小林孝成, 織田浩司, 清木興介, Eunmi Kim, 木村享史, 菅原一幸, 山田修平
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-29
• [Presentation] がん細胞の肺転移に関わる硫酸化グリコサミノグリカンの受容体の同定 (2012)
  • Author(s): 高橋潤, 水本秀二, 菅原一幸
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-29
• [Presentation] グルクロン酸転移酵素-I の変異によって引き起こされる新規ラーセン様症候群 (2012)
  • Author(s): 水本秀二, Sevjidmaa Baasanjav, Lihadh Al-Gazali, 橋口太志, Bjoern Fischer, Denise Horn, Dominik Seelow, Bassam R. Ali, Samir A.A. Aziz, Ruth Langer, Ahmed A.H. Saleh, Christian Becker, Gudrun Nürnberg, Vincent Cantagrel, Joseph G. Gleeson, Delphine Gomez, Jean-Baptiste Michel, Sigmar Stricker, Tom H. Lindner, Peter Nürnberg, Stefan Mundlos, Katrin Hoffmann, 菅原一幸
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-29
• [Presentation] グルクロン酸転移酵素-Iの変異によって引き起こされる新規ラーセン様症候群 (2012)
  • Author(s): 水本秀二, Sevjidmaa Baasanjav, Lihadh Al-Gazali, 橋口太志, Bjoern Fischer, Denise Horn, Dominik Seelow, Bassam R.Ali, Samir A.A.Aziz, Ruth Langer, Ahmed A.H.Saleh, Christian Becker, Gudrun Nurnberg, Vincent Cantagrel, Joseph G.Gleeson, Delphine Gomez, Jean-Baptiste Michel, Sigmar Stricker, Tom H.Lindner, Peter Nurnberg, Stefan Mundlos, Katrin Hoffmann, 菅原一幸
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-29
• [Presentation] Shrimp head heparan sulfate with unique structure and function (2012)
  • Author(s): Yoshiki Hama, Chen Jiancheng, Ajaya Kumar Shetty, Takanari Kobayashi, Hiroshi Oda, Kosuke Seiki, Eunmi Kim, Takashi Kimura, Kazuyuki Sugahara, Shuhei Yamada
  • Organizer: The IGP International Symposium 2012
  • Place of Presentation: Hokkaido Univ. (Sapporo)
  • Year and Date: 2012-03-02
• [Presentation] Receptor for Advanced Glycation End products (RAGE)は肺転移に関わるがん細胞表面のグリコサミノグリカン受容体として機能する (2012)
  • Author(s): 高橋潤, 水本秀二, 菅原一幸
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡市)
• [Presentation] Receptor for Advanced Glycation End-products (RAGE) Functions as a Receptor for Pulmonary Metastasis Mediated by Sulfated Glycosaminoglycans (2012)
  • Author(s): Kazuyuki Sugahara
  • Organizer: 2nd International Anatomical Sciences and Cell Biology Conference/36th Annual Conference of the Anatomy Association of Thailand
  • Place of Presentation: Chiang Mai Univ. (Thailand)
  • Invited
• [Presentation] Receptor for Advanced Glycation End products (RAGE)は肺転移に関わるがん細胞表面のグリコサミノグリカン受容体として機能する (2012)
  • Author(s): ○高橋潤, 水本秀二 , 菅原一幸
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡)
• [Presentation] Involvement of HNK-1 Sulfotransferase in the Biosynthesis of Chondroitin Sulfate and the GlcUA(3-O-sulfate)-Gal-Gal-Xyl Tetrasaccharide Found in a-Thrombomodulin from Human Urine (2011)
  • Author(s): T. Hashiguchi, S. Mizumoto, Y. Nishimura, J. Tamura, ○S. Yamada, K. Sugahara
  • Organizer: 7th International Conference on Proteoglycans
  • Place of Presentation: Q STATION (Australia)
  • Year and Date: 2011-10-18
• [Presentation] Loss-of-function mutation of glucuronyltransferase-I causes Larsen-like syndrome (2011)
  • Author(s): S. Baasanjav, L. Al-Gazali, T. Hashiguchi, ○S. Mizumoto, B. Fischer, D. Horn, D. Seelow, B. R. Ali, S.A.A. Aziz, R. Langer, A. A.H. Saleh, C. Becker, G. Nürnberg, V. Cantagrel, J. G. Gleeson, D. Gomez, J.-B. Michel, S. Stricker, T. H. Lindner, P. Nürnberg, K. Sugahara, S. Mundlos, K. Hoffmann
  • Organizer: 7th International Conference on Proteoglycans
  • Place of Presentation: Q STATION (Australia)
  • Year and Date: 2011-10-18
• [Presentation] Characterization of a receptor for glycosaminoglycans that mediate experimental lung metastasis (2011)
  • Author(s): K.Sugahara, S.Mizumoto, J.Takahashi
  • Organizer: 7th International Conference on Proteoglycans
  • Place of Presentation: QSTATION (Australia)(招待講演)
  • Year and Date: 2011-10-18
• [Presentation] Loss-of-function mutation of glucuronyltransferase-I causes Larsen-like syndrome (2011)
  • Author(s): S.Baasanjav, L.Al-Gazali, T.Hashiguchi, 〇S.Mizumoto, B.Fischer, D.Horn, D.Seelow, B.R.Ali, S.A.A.Aziz, R.Langer, A.A.H.Saleh, C.Becker, G.Nurnberg, V.Cantagrel, J.G.Gleeson, D.Gomez, J.-B.Michel, S.Stricker, T.H.Lindner, P.Nurnberg, K.Sugahara, S.Mundlos, K.Hoffmann
  • Organizer: 7th International Conference on Proteoglycans
  • Place of Presentation: QSTATION (Australia)
  • Year and Date: 2011-10-18
• [Presentation] Involvement of HNK-1 Sulfotransferase in the Biosynthesis of Chondroitin Sulfate and the GlcUA(3-0-sulfate)-Gal-Gal-Xyl Tetrasaccharide Found in a-Thrombomodulin from Human Urine (2011)
  • Author(s): T.Hashiguchi, S.Mizumoto, Y.Nishimura, J.Tamura, 〇S.Yamada, K.Sugahara
  • Organizer: 7th International Conference on Proteoglycans
  • Place of Presentation: QSTATION (Australia)
  • Year and Date: 2011-10-18
• [Presentation] A deficiency of glycosaminoglycans causes cardiac and joint disorders (2011)
  • Author(s): Sevjidmaa Baasanjav, Lihadh Al-Gazali, Taishi Hashiguchi, ○ Shuji Mizumoto, Bjoern Fischer, Denise Horn, Dominik Seelow, Bassam R. Ali, Samir A.A. Aziz, Ruth Langer, Ahmed A.H. Saleh, Christian Becker, Gudrun Nürnberg, Vincent Cantagrel, Joseph G. Gleeson, Delphine Gomez, Jean-Baptiste Michel, Sigmar Stricker, Tom H. Lindner, Peter Nürnberg, Kazuyuki Sugahara, Stefan Mundlos, Katrin Hoffmann
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2011-09-24
• [Presentation] A deficiency of glycosaminoglycans causes cardiac and joint disorders (2011)
  • Author(s): Sevjidmaa Baasanjav, Lihadh Al-Gazali, Taishi Hashiguchi, Shuji Mizumoto, Bjoern Fischer, Denise Horn, Dominik Seelow, Bassam R.Ali, Samir A.A.Aziz, Ruth Langer, Ahmed A.H.Saleh, Christian Becker, Gudrun Nurnberg, Vincent Cantagrel, Joseph G.Gleeson, Delphine Gomez, Jean-Baptiste Michel, Sigmar Stricker, Tom H.Lindner, Peter Nurnberg, Kazuyuki Sugahara, Stefan Mundlos, Katrin Hoffmann
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-09-24
• [Presentation] Involvement of chondroitin sulphate E-type structure in the experimental metastasis of the Lewis lung carcinoma cell line (2011)
  • Author(s): Kazuyuki Sugahara
  • Organizer: The 3rd FEBS-MPST Advanced Lecture Course on Matrix Pathobiology, Signaling and Molecular Targets
  • Place of Presentation: Spetses Hotel and the Anargyrios College (Greece)
  • Year and Date: 2011-09-06
  • Invited
• [Presentation] Involvement of chondroitin sulphate E-type structure in the experimental metastasis of the Lewis lung carcinoma cell line (2011)
  • Author(s): K.Sugahara
  • Organizer: The 3rd FEBS-MPST Advanced Lecture Course on Matrix Pathobiology, Signaling and Molecular Targets (FEBS-MPST2011)
  • Place of Presentation: Spetses Hotel and Anargyrios College (Greece)(招待講演)
  • Year and Date: 2011-09-06
• [Presentation] ルイス肺癌細胞表面上のコンドロイチン硫酸E 構造の発現低下による転移抑制 (2011)
  • Author(s): 水本秀二, 渡邊基夫, 山田修平, 菅原一幸
  • Organizer: 日本薬学会北海道支部第136回例会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-05-21
• [Presentation] エビ頭部由来へパラン硫酸の構造と機能の研究 (2011)
  • Author(s): 陳建成, 羽磨紀城, Ajaya Kumar Shetty, 小林孝成, 織田浩司, 清木興介, 木村享史, 高橋尚徳, 左一八, 鈴木隆, 鈴木康夫, ○山田修平, 菅原一幸
  • Organizer: 日本薬学会北海道支部第136回例会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-05-21
• [Presentation] エビ頭部由来ヘパラン硫酸の構造と機能の研究 (2011)
  • Author(s): 陳建成、羽磨紀城、Ajaya Kumar Shetty、小林孝成、織田浩司、清木興介、木村享史、高橋尚徳、左一八、鈴木隆、鈴木康夫、〇山田修平、菅原一幸
  • Organizer: 日本薬学会北海道支部第136回例会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-05-21
• [Presentation] ルイス肺癌細胞表面上のコンドロイチン硫酸E構造の発現低下による転移抑制 (2011)
  • Author(s): 水本秀二、渡邊基夫、山田修平、菅原一幸
  • Organizer: 日本薬学会北海道支部第136回例会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-05-21
• [Presentation] Analysis of the expression pattern of dermatan sulfate epimerase-2, which is involved in the bipolar disorder, in the mouse brain during postnatal development (2011)
  • Author(s): Chizuru Akatsu, Shuji Mizumoto, Tomoyuki Kaneiwa, Marco Maccarana, Anders Malmström, Shuhei Yamada, ○Kazuyuki Sugahara
  • Organizer: The 9th International Symposium for Future Drug Discovery and Medical Care
  • Place of Presentation: Hokkaido Univ. (Sapporo)
• [Presentation] エビ頭部に由来するヘパラン硫酸の構造と生物活性 (2011)
  • Author(s): 羽磨紀城, 陳建成, Ajaya Kumar Shetty, 小林孝成, 織田浩司, 清木興介, Eunmi Kim, 木村享史, 菅原一幸, 山田修平
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: 長岡リリックホール(長岡市)
• [Presentation] ルイス肺癌細胞表面上のコンドロイチン硫酸E 構造の発現低下による転移抑制 (2011)
  • Author(s): 渡邊基夫, 水本秀二, 山田修平, ○菅原一幸
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: 長岡リリックホール(長岡市)
• [Presentation] ルイス肺癌細胞表面上のコンドロイチン硫酸E構造の発現低下による転移抑制 (2011)
  • Author(s): 渡邊基夫、水本秀二、山田修平、菅原一幸
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: 長岡リリックホール(新潟県)
• [Presentation] エビ頭部に由来するヘパラン硫酸の構造と生物活性 (2011)
  • Author(s): 羽磨紀城、陳建成、Ajaya Kumar Shetty、小林孝成、織田浩司、清木興介、Eunmi Kim、木村享史、菅原一幸、山田修平
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: 長岡リリックホール(新潟県)
• [Presentation] Mass preparation of oligosaccharides with intestinal absorption capacity by hydrolysis of chondroitin sulfate polysaccharides with subcritical water microreaction system
  • Author(s): Kazuyuki Sugahara, Shuhei Yamada, Shuji Mizumoto, Keiichiro Matsushima, Haruo Ura, and Nobuyuki Miyamoto
  • Organizer: The 13th International Conference on Functional and Medical Foods with Bioactive Compounds: Science and Practical Applications”
  • Place of Presentation: The Kyoto Prefectural University of Medicine (京都)
• [Presentation] Receptor for Advanced Glycation End-products (RAGE) Functions as Receptor for Sulfated Glycosaminoglycans (GAGs): Anti-RAGE Antibody or Sulfated GAGs Inhibit Lung Metastasis of Tumor Cells
  • Author(s): Kazuyuki SUGAHARA, Jun TAKAHASHI, Shuji MIZUMOTO
  • Organizer: 22nd International Symposium on Glycoconjugates
  • Place of Presentation: Dalian Institute of Chemical Phisics, Chinese Academy of Sciences(中国)
• [Presentation] Wobble chondroitin sulfate oligosaccharide motifs and mass production of oligosaccharides
  • Author(s): Kazuyuki Sugahara
  • Organizer: The 21st Symposium on Glycosaminoglycans
  • Place of Presentation: Loveno, Lake Como(イタリア）
  • Invited
• [Presentation] Mechanism of glycosaminoglycan-mediated tumor metastasis
  • Author(s): Kazuyuki Sugahara and Shuji Mizumoto
  • Organizer: The 27th International Carbohydrate Symposium
  • Place of Presentation: Indial Institute of Science (インド)
  • Invited
• [Presentation] Receptor for Advanced Glycation End Products (RAGE)は癌細胞の肺転移に関わるコンドロイチン硫酸の受容体として機能する
  • Author(s): ○水本秀二、高橋潤、菅原一幸
  • Organizer: 日本薬学会北海道支部第138回例会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
• [Presentation] がんの肺転移に関わるグリコサミノグリカン受容体(RAGE)の同定
  • Author(s): ○高橋潤、水本秀二、菅原一幸
  • Organizer: 第49 回日本生化学会北海道支部例会
  • Place of Presentation: 北海道大学(札幌市）
• [Presentation] Characterization of a receptor for glycosaminoglycans that mediate experimental lung metastasis
  • Author(s): Kazuyuki Sugahara
  • Organizer: The 15th Gordon Research Conference on Proteoglycans
  • Place of Presentation: Proctor Academy(米国)
  • Invited
• [Presentation] 糖鎖生物学による癌の肺転移の分子メカニズムの解明 -抗癌剤開発に向けての洞察-
  • Author(s): Kazuyuki Sugahara
  • Organizer: 第１６回未来創薬・医療イノベーションセミナー
  • Place of Presentation: 北海道大学(札幌市）
  • Invited
• [Presentation] RAGEは癌細胞の肺転移に関わるコンドロイチン硫酸の受容体として機能する
  • Author(s): ○ 水本秀二, 高橋潤, 菅原一幸
  • Organizer: 第３１回日本糖質学会年会
  • Place of Presentation: 鹿児島市民ホール(鹿児島市）
• [Presentation] Receptor for Advanced Glycation End-products (RAGE) functions as a receptor for glycosaminoglycans involved in pulmonary metastasis
  • Author(s): ◯Shuji MIZUMOTO, Jun TAKAHASHI, Kazuyuki SUGAHARA
  • Organizer: The 10th International Symposium for Future Drug Discovery and Medical Care
  • Place of Presentation: Hokaido Univ. (Sapporo)
• [Presentation] GAG signals involved in pulmonary metastasis of tumor cells
  • Author(s): Kazuyuki Sugahara
  • Organizer: The 20th Symposium on glycosaminoglycans
  • Place of Presentation: Villa VigoniVilla(イタリア）
• [Presentation] Receptor for AdvancedGlycation End-products (RAGE) Functions as a Receptor for Pulmonary Metastasis Mediated by Sulfated Glycosaminoglycans
  • Author(s): Kazuyuki SUGAHARA
  • Organizer: 2nd International Anatomical Sciences and Cell Biology Conference & 36th Annual Conference of the Anatomy Association of Thailand
  • Place of Presentation: Chiang Mai Grandview Hotel & Convention Center(タイ)
  • Invited
• [Book] 「ISCSM2011 Chondroitin sulfate E-type structure at tumor cell surface is involved in experimental metastasis (Chapter 3)」, Advances in Experimental Medicine and Biology "Biochemical Roles of Eukaryotic Cell Surface Macromolecules", (P. R. Sudhakaran and A. Surolia, eds) (2012)
  • Author(s): Kazuyuki Sugahara, Shuji Mizumoto
  • Publisher: Springer, New York
• [Book] 「Bone and Skin Disorders Caused by a Disturbance in the Bioynthesis of Chondroitin Sulfate and Dermatan Sulfate 」, Extracellular matrix : Pathobiology and signaling , (N. Karamanos ed.) (2012)
  • Author(s): Shuji Mizumoto, Kazuyuki Sugahara
  • Publisher: Walter De Gruyter, Berlin, Germany
• [Remarks] 研究業績
  • URL: http://www.lfsci.hokudai.ac.jp/labs/infmem/sugaken/lonbun.html
• [Remarks]
  • URL: http://www.lfsci.hokudai.ac.jp/labs/infmem/index.html