Generation and characterization of genetically-altered mice lacking a novel vitamin K2 synthesis enzyme Ubiad1
Project/Area Number |
23390022
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Kobe Pharmaceutical University |
Principal Investigator |
OKANO TOSHIO 神戸薬科大学, 薬学部, 教授 (20131542)
|
Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Kimie 神戸薬科大学, 薬学部, 准教授 (90309435)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
|
Keywords | 遺伝子改変動物 / ビタミンK2 / 合成酵素 / UBIAD1 / 全身性ノック・アウト / コンデショナル・ノックアウト / 神経 / 骨 / ビタミンK2 / UBIAD1 / 全身性ノックアウト / コンディショナル・ノックアウト / ヒト / 遺伝子欠損動物 / CAG-Creヘテロマウス / CAG-Flpヘテロマウス |
Research Abstract |
It was demonstrated that tissue vitamin K2 originates from oral vitamin K1 by a mechanism that involves the release of vitamin K3 from vitamin K1 in the intestine followed by delivery of vitamin K3 through the mesenteric lymphatic system and blood circulation to tissues, and then reduced by an unknown redox enzyme in the tissues and converted into vitamin K2 by the prenylating enzyme Ubiad1. To investigate the function of ubiad1, we attempted to generate mice lacking Ubiad1 by gene targeting, but it was found that Ubiad1-deficient mouse embryos failed to survive beyond embryonic day 7.5. We have succeeded in generating hematopoietic and nerve tissue -specific, bone specific- or neuron-specific Ubiad1-deficient mice by intercrossing Ubiad1 flox/flox mice with Nestin-Cre expressing mice, Osterix-Cre expressing mice or Synapsin-Cre expressing mice.
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Report
(4 results)
Research Products
(142 results)
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[Journal Article] Liver-specific γ-glutamyl carboxylase-1 deficient mice display bleeding diathesis and short life span.2014
Author(s)
Azuma K, Tsukui T, Ikeda K, Shiba S, Nakagawa K, Okano T, Urano T, Horie-Inoue K, Ouchi Y, Ikawa M, Inoue S
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Journal Title
PLoS ONE
Volume: 9
Issue: 2
Pages: e88643-e88643
DOI
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Peer Reviewed
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[Presentation] 脂溶性ビタミン2011
Author(s)
岡野登志夫
Organizer
第2回近畿地区ビタミン懇話会
Place of Presentation
スペースアルファ神戸(兵庫県)
Year and Date
2011-11-27
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[Presentation] 栄養と薬2011
Author(s)
岡野登志夫
Organizer
兵庫県栄養士会主催生涯学習講演
Place of Presentation
神戸女子大学(兵庫県)
Year and Date
2011-09-03
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