| Project/Area Number |
23390032
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Showa Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ANAN Yasumi 昭和薬科大学, 薬学部, 講師 (40403860)
KOIZUMI Shinji 独立行政法人労働安全衛生総合研究所, 研究企画調整部, 特任研究員 (80183325)
徳本 真紀 昭和薬科大学, 薬学部, 助教 (90614339)
八幡 紋子 昭和薬科大学, 薬学部, 助教 (00384636)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | セレン / スペシエーション / セレノシアネート / ICP-MS / Q-TOF / ESI-MS / テルル / セレン糖 / ESI-MS-MS / セレノシアン酸 / HPLC / LC-MS / セレノプロテイン / メタロチオネイン |
|---|
| Research Abstract |
When human hepatoma HepG2 cells were exposed to sodium selenite, an unknown selenium metabolite was detected in the cytosolic fraction by HPLC coupled with an inductively coupled plasma mass spectrometer (ICP-MS). The unknown selenium metabolite was also detected in the mixture of HepG2 homogenate and sodium selenite in the presence of exogenous glutathione (GSH). The unknown selenium metabolite was identified as selenocyanate by electrospray ionization mass spectrometry (ESI-MS) and ESI quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS). Selenocyanate was less toxic to HepG2 cells than selenite or cyanide, suggesting that it was formed to reduce the toxicity of selenite. On the other hand, selenocyanate could be assimilated to selenoproteins and selenometabolites in rats. Consequently, selenite was metabolized to selenocyanate to temporarily ameliorate its toxicity, and selenocyanate acted as an intrinsic selenium pool.
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