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Analysis of cohesin model in CYP3A4 gene and individual phenotypic status

Research Project

Project/Area Number 23390035
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical pharmacy
Research InstitutionKyushu University

Principal Investigator

IEIRI ICHIRO  九州大学, 薬学研究科(研究院), 教授 (60253473)

Co-Investigator(Kenkyū-buntansha) HIROTA Takeshi  九州大学, 大学院・薬学研究院, 助教 (80423573)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywords遺伝子 / 発現制御 / 薬剤反応性 / CYP3A4 / 遺伝子空間的転写モデル / 個人差解明 / 臨床試験 / 立体空間的発現制御 / メチル化 / CpGアイランド
Research Abstract

We showed that DNA methylation in CYP3A4 enhancer region is critically involved in determining the individual hepatic CYP3A4 mRNA level. Chromosome Conformation Capture (3C) assay suggested long-range cis-interaction between the enhancer region and the transcription start site. It is well known that miR-122 shows specific expression in human liver and asialoglycoprotein receptor (ASGP-R) is a liver-specific protein. We established the method using immuno-precipitation with polyclonal antibody against ASGP-R, to isolate the circulating cells showing miR-122 expression from peripheral blood. These results suggest that the isolated cells is from human liver cells. The established method can be used for the detection of human liver DNA methylation status using peripheral blood.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (7 results)

All 2013 2011 Other

All Presentation (6 results) Remarks (1 results)

  • [Presentation] エピジェネティック制御機構に基づくCYP3A4 遺伝子発現制御メカニズムの解析2013

    • Author(s)
      藤田 麻里絵, 江口 駿介, 廣田 豪, 家入 一郎
    • Organizer
      第30回日本薬学会九州支部大会
    • Year and Date
      2013-12-08
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒトCYP3A4 遺伝子発現のゲノム空間構造解析に基づく個人差解明2013

    • Author(s)
      江口 駿介, 廣田 豪, 舞 彩華, 家入一郎
    • Organizer
      日本薬学会 第133年会
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] エピジェネティック制御機構に基づくCYP3A4遺伝子発現制御メカニズムの解析2013

    • Author(s)
      藤田 麻里絵、江口 駿介、廣田 豪、家入 一郎
    • Organizer
      第30回日本薬学会九州支部大会
    • Place of Presentation
      長崎
    • Related Report
      2013 Annual Research Report
  • [Presentation] CYP3A4 遺伝子発現の個人差解明を指向したエピジェネティック解析2011

    • Author(s)
      江口 駿介, 廣田 豪, 家入 一郎
    • Organizer
      第28回日本薬学会九州支部大会
    • Year and Date
      2011-12-10
    • Related Report
      2013 Final Research Report
  • [Presentation] CYP3A4遺伝子発現の個人差解明を指向したepigenetic解析2011

    • Author(s)
      江口駿介
    • Organizer
      日本薬学会九州支部大会
    • Place of Presentation
      福岡
    • Year and Date
      2011-12-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトCYP3A4遺伝子発現のゲノム空間構造解析に基づく個人差解明

    • Author(s)
      家入 一郎
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Remarks] 九州大学大学院薬学研究院薬物動態学分野Publication

    • URL

      http://doutai.phar.kyushu-u.ac.jp/6.publications/publications.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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