Mechanistic analysis of the circadian clock development in mammals
| Project/Area Number |
23390051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YAGITA Kazuhiro 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90324920)
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| Co-Investigator(Kenkyū-buntansha) |
INOKAWA Hitoshi 京都府立医科大学, 医学研究科, 講師 (40285250)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | 体内時計 / 細胞分化 / ES細胞 / 発生 / 概日リズム / 幹細胞 / 分化誘導 / RNA-seq / イメージング |
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| Outline of Final Research Achievements |
In mammals, the emergence of circadian clock oscillation gradually occurs during the developmental process. However, developmental mechanisms of circadian clock have not been well understood.
We have shown that the circadian clock oscillation is not detected in the mouse embryonic stem (ES) cells. We also showed that the apparent circadian clock oscillation is induced during the differentiation culture of mouse ES cells without maternal entraining factors. These results demonstrate that the intrinsic program controls the circadian oscillator formation during the differentiation process of ES cells in cultural condition.
To understand the circadian clock development in mammals, we performed ES cell-based screening to find out factors contributing to the clock formation mechanisms, and found Dnmt1 as an essential factor for circadian clock development. Epigenetic programming and following organization of transcriptional network may be essential for circadian clock development in mammals.
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Report
(5 results)
Research Products
(33 results)
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[Journal Article] Parathyroid hormone resets the cartilage circadian clock of the organ-cultured murine femur.2015
Author(s)
Okubo N, Fujiwara H, Minami Y, Kunimoto T, Hosokawa T, Umemura Y, Inokawa H, Asada M, Oda R, Kubo T, Yagita K.
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Journal Title
Acta Orthopedica
Volume: 13
Issue: 5
Pages: 1-5
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] An in vitro ES cell-based clock recapitulation assay model identifies CK2a as an endogenous clock regulator2013
Author(s)
Umemura Y, Yoshida J, Wada M, Tsuchiya Y, Minami Y, Watanabe H, Kondoh G, Takeda J, Inokawa H, Horie K, Yagita K.
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Journal Title
PLoS One
Volume: 8
Issue: 6
Pages: e67241-e67241
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 生殖と概日リズム.2014
Author(s)
八木田和弘
Organizer
第４３回日本女性心身医学会学術集会
Place of Presentation
京都
Year and Date
2014-08-10
Related Report
Invited
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