| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
In mammals, the emergence of circadian clock oscillation gradually occurs during the developmental process. However, developmental mechanisms of circadian clock have not been well understood.
We have shown that the circadian clock oscillation is not detected in the mouse embryonic stem (ES) cells. We also showed that the apparent circadian clock oscillation is induced during the differentiation culture of mouse ES cells without maternal entraining factors. These results demonstrate that the intrinsic program controls the circadian oscillator formation during the differentiation process of ES cells in cultural condition.
To understand the circadian clock development in mammals, we performed ES cell-based screening to find out factors contributing to the clock formation mechanisms, and found Dnmt1 as an essential factor for circadian clock development. Epigenetic programming and following organization of transcriptional network may be essential for circadian clock development in mammals.
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