Regulatory mechanism of FDC activation via Fca/mR

• Project/Area Number: 23390091
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: University of Tsukuba
• Principal Investigator: HONDA Shinichiro 筑波大学, 医学医療系, 准教授 (60360640)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 濾胞樹状細胞 / IgM / Fc受容体 / Fc 受容体 / IgA

Research Abstract

Follicular dendritic cells (FDCs) capture and retain antigen for long period to activate cognate B cells, leading to germinal center formation. We established a novel method for FDCs isolation from lymphoid organs of naive mice. Surprisingly, analysis with this method showed that FDCs internalized capturing antigen via Fca/mR. Aa a result of this internalization, antigen amount on FDCs cells surface was decreased. These results suggested that Fca/mR regulates antigen amount on FDCs cell surface, leading to the regulation of B cells activation and germinal center formation.

Research Products

• [Journal Article] Involvement of Fcα/μR (CD351) in autoantibody production (2013)
  • Author(s): Yoshizawa Y, Honda S, Shibuya A
  • Journal Title: Mol Immunol Volume: 57(2) Issue: 2 Pages: 216-219
  • DOI: 10.1016/j.molimm.2013.10.002
  • NAID: 120007130578
  • Peer Reviewed
• [Journal Article] Molecular characterization of the dimer formation of Fcα/μ receptor (CD351) (2013)
  • Author(s): Takagaki K, Satoh K, Honda S, Shibuya A
  • Journal Title: Mol Immunol Volume: 56(1-2) Issue: 1-2 Pages: 23-27
  • DOI: 10.1016/j.molimm.2013.04.003
  • NAID: 120007136867
  • Peer Reviewed
• [Journal Article] Obesity-associated autoantibody production requires AIM to retain IgM immune complex on follicular dendritic cells (2013)
  • Author(s): Arai, S., et al.
  • Journal Title: Cell Rep. Volume: 3 Issue: 4 Pages: 1-12
  • DOI: 10.1016/j.celrep.2013.03.006
  • Peer Reviewed
• [Journal Article] Apoptotic cells suppress mast cell inflammatory responses via the CD300a immunoreceptor. (2012)
  • Author(s): Nakahashi-Oda, C., Tahara-Hanaoka, S., Shoji, M., Okoshi, Y., Nakano-Yokomizo, T., Ohkohchi, N., Yasui, T., Kikutani, H., Honda, S., Shibuya, K., Nagata, S. and Shibuya, A.
  • Journal Title: J Exp. Med. Volume: 209 Issue: 8 Pages: 1493-1503
  • DOI: 10.1084/jem.20120096
  • Peer Reviewed
• [Journal Article] Identification of phosphatidylserine as a ligand for the CD300a immunoreceptor (2012)
  • Author(s): Chigusa Nakahashi-Oda, et.al.
  • Journal Title: BBRC Volume: 417 Issue: 1 Pages: 646-650
  • DOI: 10.1016/j.bbrc.2011.12.025
  • Peer Reviewed
• [Journal Article] Isolation and characterization of naive follicular dendritic cells (2012)
  • Author(s): Kenta Usui
  • Journal Title: Molecular Immunology Volume: 50 Issue: 3 Pages: 172-176
  • DOI: 10.1016/j.molimm.2011.11.010
  • Peer Reviewed
• [Journal Article] Isolation and characterization of naive follicular dendritic cells (2012)
  • Author(s): Usui, et al.
  • Journal Title: Mol Immunol Volume: 50 Pages: 172-176
  • Peer Reviewed
• [Journal Article] The immunoreceptor adapter protein DAP12 suppresses B lymphocyte-driven adaptive immune responses (2011)
  • Author(s): Takako Nakano-Yokomizo, et.al.
  • Journal Title: J Exp Med Volume: 8 Issue: 8 Pages: 1661-1671
  • DOI: 10.1084/jem.20101623
  • Peer Reviewed
• [Journal Article] Fcα/μ を介した免疫制御 (2011)
  • Author(s): 本多伸一郎, 渋谷 彰
  • Journal Title: 臨床免疫・アレルギー科 Volume: 55(5) Pages: 552-558
  • URL: http://www.kahyo.com/item/M201105-555
• [Journal Article] Identification of phosphatidylserine as a ligand for the CD300a immunoreceptor (2011)
  • Author(s): Nakahashi-oda, et al.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 417 Pages: 646-650
  • Peer Reviewed
• [Journal Article] The immunoreceptor adapter protein DAP12 suppresses B lymphocyte-driven adaptive immune responses (2011)
  • Author(s): Nakano-Yokomizo, et al.
  • Journal Title: J Exp Med Volume: 208 Pages: 1661-1671
  • Peer Reviewed
• [Presentation] Involvement of CD300a in the pathogenesis of DSS-induced colitis (2013)
  • Author(s): Totsuka N, Kim YG, Tahara-Hanaoka S, Nakahashi-Oda C, Honda S, Shibuya K, Shibuya A
  • Organizer: 第42回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2013-12-13
• [Presentation] Fcα/μR (CD351) regulates inflammatory responses of marginal zone B cells against experimental septic shock (2013)
  • Author(s): Honda S, Yoshizawa Y, Sato K, Fujimoto M, Nakahashi-Oda C, Tahara-Hanaoka S, Shibuya K, Shibuya A
  • Organizer: 第42回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2013-12-12
• [Presentation] Obesity-associated autoantibody production requires AIM to retain IgM immune complex on follicular dendritic cells (2013)
  • Author(s): Arai S, Honda S, Shibuya A, Li Q-Z, Miyazaki T
  • Organizer: 第42回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2013-12-11
• [Presentation] Fcα/μR (CD351) regulates inflammatory responses of marginal zone B cells against experimental septic shock (2013)
  • Author(s): Honda S, Satoh K, Matsuoka Y, Matsushita T, Fujimoto M, Shibuya A
  • Organizer: 15th International Congress of Immunology. Mico Milano Congressi
  • Place of Presentation: Milan, Italy
  • Year and Date: 2013-08-23
• [Presentation] Apoptotic cells suppress mast cell inflammatory responses via the CD300a immunoreceptor (2012)
  • Author(s): Nakahashi-Oda C, Tahara-Hanaoka S, Yasui T, Kikutani H, Honda S, Shibuya K, Nagata S, Shibuya A
  • Organizer: 第41回日本免疫学会国際シンポジウム
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 2012-12-06
• [Presentation] Follicular dendritc cells (FDC) internalized IgM-coated antigen via Fc receptor for IgA/IgM (Fcα/μR; CD351) (2012)
  • Author(s): Sato K, Usui K, Honda S, Yoshizawa Y, Shibuya A
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 2012-12-05
• [Presentation] Follicular dendritc cells (FDC) internalized IgM-coated antigen via Fc receptor for IgA/IgM (Fcα/μR; CD351) (2012)
  • Author(s): Sato K
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 神戸国際会議場（兵庫）
• [Presentation] MAIR-II(CD300d) on Ly-6C high inflammatory monocytes plays a crucial role in inflammatory responses in a murine sepsis model (2011)
  • Author(s): 戸塚直也, 田原聡子, 永井恵, 小田(中橋)ちぐさ, 人見香織, 栗田尚樹, 本多伸一郎, 渋谷和子, 渋谷彰
  • Organizer: 第40回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2011-11-29
• [Presentation] ナイーブマウス脾臓からの FDC の単離 (2011)
  • Author(s): 臼井健太, 本多伸一郎, 吉澤勇一, 田原聡子, 渋谷和子, 渋谷彰
  • Organizer: 第40回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2011-11-29
• [Presentation] Iga/IgM受容体, Fcα/μ 受容体の二量体形成の解析 (2011)
  • Author(s): 高垣香菜, 本多伸一郎, 臼井健太, 吉澤勇一, 渋谷和子, 田原聡子, 渋谷彰
  • Organizer: 第40回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2011-11-29
• [Presentation] ナイーブマウス牌臓からのFDCの単離 (2011)
  • Author(s): 臼井健太, 他
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ・千葉
  • Year and Date: 2011-11-29
• [Presentation] IgA/IgM受容体、Fcα/μ受容体の二量体形成の解析 (2011)
  • Author(s): 高垣香菜, 他
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ・千葉
  • Year and Date: 2011-11-29
• [Presentation] MAIR-II(CD300d) on Ly-6Chigh inflammatory monocytes plays a crucial role in inflamtnato res onses in a murine sepsis model (2011)
  • Author(s): 戸塚直也, 他
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ・千葉
  • Year and Date: 2011-11-29
• [Presentation] An immunoreceptor adapter protein, DAP12, suppresses adaptive immune responses mediated by B lymphocytes (2011)
  • Author(s): 小田(中橋)ちぐさ, 他
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ・千葉
  • Year and Date: 2011-11-29
• [Presentation] The role of CD155, a novel co-stimulatory molecule in CD4+ T cell, in contact dermatitis (2011)
  • Author(s): 山下由美, 他
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ・千葉
  • Year and Date: 2011-11-29
• [Presentation] The role of CD155, a novel co-stimulatory molecule in CD4+ T cell, in contact dermatitis (2011)
  • Author(s): 山下由美, Wang Yhan , 吉岡文, 鍋倉宰, 田原聡子, 本多伸一郎, 渋谷彰, 渋谷和子
  • Organizer: 第40回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2011-11-27
• [Presentation] 濾胞状樹状細胞上の IgA 受容体を介した粘膜免疫応答制御機構の解析 (2011)
  • Author(s): 本多伸一郎
  • Organizer: 平成 23年度第1回班会議
  • Place of Presentation: 京都大学医学部芝蘭会館(京都)
  • Year and Date: 2011-07-19
• [Presentation] Fca/mR (CD351) regulates inflammatory responses of marginal zone B cells against experimental septic shock.
  • Author(s): Honda S, Satoh K, Matsuoka Y, Matsushita T, Fujimoto M, Shibuya A.
  • Organizer: 15th International Congress of Immunology
  • Place of Presentation: Mico Milano Congressi, Milan, Italy
• [Presentation] Fca/mR (CD351) regulates inflammatory responses of marginal zone B cells against experimental septic shock.
  • Author(s): Honda S, Yoshizawa Y, Sato K, Fujimoto M, Nakahashi-Oda C, Tahara-Hanaoka S, Shibuya K, Shibuya A.
  • Organizer: 第42回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Involvement of CD300a in the pathogenesis of DSS-induced colitis.
  • Author(s): Totsuka N, Kim YG, Tahara-Hanaoka S, Nakahashi-Oda C, Honda S, Shibuya K, Shibuya A.
  • Organizer: 第42回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Obesity-associated autoantibody production requires AIM to retain IgM immune complex on follicular dendritic cells
  • Author(s): Arai S, Honda S, Shibuya A, Li Q-Z, Miyazaki T..
  • Organizer: 第42回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Remarks] つくばリポジトリ
  • URL: http://www.tulips.tsukuba.ac.jp/dspace/simple-search?query=%E6%9C%AC%E5%A4%9A+%E4%BC%B8%E4%B8%80%E9%83%8E
• [Remarks] TRIOS
  • URL: http://trios.tsukuba.ac.jp/researcher/0000001684
• [Remarks] つくばリポジトリ
  • URL: http://www.tulips.tsukuba.ac.jp/dspace/simple-search?query=%E6%9C%AC%E5%A4%9A+%E4%BC%B8%E4%B8%80%E9%83%8E
• [Remarks] TRIOS
  • URL: http://trios.tsukuba.ac.jp/researcher/0000001684