| Project/Area Number |
23390104
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAMITANI Shigeki 大阪大学, 微生物病研究所, 助教 (60379089)
ABE Hiroyuki 大阪大学, 微生物病研究所, 助教 (00379265)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | 百日咳 / 気管支敗血症菌 / 咳嗽 / 百日咳菌 / 咳嗽発作 |
|---|
| Research Abstract |
To understand the mechanism of paroxysmal cough observed in whooping cough, Bordetella pertussis infection, we established an animal model that reproduce the paroxysmal cough by using B. bronchiseptica, which is closely related to B. pertussis. This model animal showed coughing after intranasal infection by 500 cfu of B. bronchiseptica. In the course of analyzing the B. bronchiseptica infection, we succeeded in isolating a mutant strain that did not induce coughing in the model animal. A comparison of the genomes of the mutant strain and the parental wild type strain revealed a single-base insertion in a gene designated crg1. A strain deficient in crg1 was found not to cause the paroxysmal cough in the animals, showing that crg1 might be involved in the paroxysmal cough. Further work is in progress to characterize the crg1 gene.
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