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Basic analysis of pathogenesis of whooping cough

Research Project

Project/Area Number 23390104
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOsaka University

Principal Investigator

HORIGUCHI Yasuhiko  大阪大学, 微生物病研究所, 教授 (00183939)

Co-Investigator(Kenkyū-buntansha) KAMITANI Shigeki  大阪大学, 微生物病研究所, 助教 (60379089)
ABE Hiroyuki  大阪大学, 微生物病研究所, 助教 (00379265)
Project Period (FY) 2011-11-18 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords百日咳 / 気管支敗血症菌 / 咳嗽 / 百日咳菌 / 咳嗽発作
Research Abstract

To understand the mechanism of paroxysmal cough observed in whooping cough, Bordetella pertussis infection, we established an animal model that reproduce the paroxysmal cough by using B. bronchiseptica, which is closely related to B. pertussis. This model animal showed coughing after intranasal infection by 500 cfu of B. bronchiseptica. In the course of analyzing the B. bronchiseptica infection, we succeeded in isolating a mutant strain that did not induce coughing in the model animal. A comparison of the genomes of the mutant strain and the parental wild type strain revealed a single-base insertion in a gene designated crg1. A strain deficient in crg1 was found not to cause the paroxysmal cough in the animals, showing that crg1 might be involved in the paroxysmal cough. Further work is in progress to characterize the crg1 gene.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (49 results)

All 2014 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (43 results) (of which Invited: 8 results) Book (1 results)

  • [Journal Article] Defining the substrate specificity of Pasteurella multocida toxin-induced deamidation of α-subunits of heterotrimeric G proteins2013

    • Author(s)
      Orth, J. H., Fester, I., Siegert, P., Weise, M., Lanner, U., Kamitani, S., Tachibana, T., Wilson, B. A., Schlosser, A., Horiguchi, Y., and Aktories,K
    • Journal Title

      FASEB J

      Volume: 27 Issue: 2 Pages: 832-842

    • DOI

      10.1096/fj.12-213900

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Swine Atrophic Rhinitis caused by Pasteurella multocida toxin and Bordetella dermonecrotic toxin2012

    • Author(s)
      Y. Horiguchi
    • Journal Title

      Current Topics in Microbiology and Immunology

      Volume: 361 Pages: 113-129

    • DOI

      10.1007/82_2012_206

    • ISBN
      9783642310164, 9783642310171
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal Structure of Clostridiumperfringensenterotoxin displays features of β-pore-forming toxins2011

    • Author(s)
      Kitadokoro, K., Nishimura, K., Kamitani, S., Fukui-Miyazaki, A., Toshima, H., Abe, H., Kamata, Y., Sugita-Konishi, Y., Yamamoto, S., Karatani, H., and Horiguchi, Y
    • Journal Title

      J Biol Chem

      Volume: 286 Issue: 22 Pages: 19549-19555

    • DOI

      10.1074/jbc.m111.228478

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq2011

    • Author(s)
      Kamitani, S., Ao, S., Toshima, H., Tachibana, T., Hashimoto, M., Kitadokoro, K., Fukui-Miyazaki, A., Abe, H., and Horiguchi, Y.
    • Journal Title

      FEBS J

      Volume: 278 Issue: 15 Pages: 2702-2712

    • DOI

      10.1111/j.1742-4658.2011.08197.x

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids2011

    • Author(s)
      Fukui-Miyazaki A, Ohnishi S, Kamitani S, Abe H, Horiguchi Y
    • Journal Title

      Microbiol Immunol

      Volume: 55 Issue: 3 Pages: 154-159

    • DOI

      10.1111/j.1348-0421.2010.00300.x

    • NAID

      10031121454

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] ブタ由来気管支敗血症菌 S798 株のゲノム解析2014

    • Author(s)
      岡田圭祐, 安倍裕順, 小椋義俊, 林 哲也, 阿部章夫, 桑江朝臣, 堀口安彦
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京江戸川区 タワーホール船堀
    • Year and Date
      2014-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] IVET-IP 法 : ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析2014

    • Author(s)
      安倍裕順, 神谷重樹, 福井理, 岡田圭祐, 堀口安彦
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京江戸川区 タワーホール船堀
    • Year and Date
      2014-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] IVET-IP法: ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析2014

    • Author(s)
      安倍 裕順
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] ブタ由来気管支敗血症菌S798株のゲノム解析2014

    • Author(s)
      岡田 圭祐
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 気管支敗血症菌が宿主体内で発現する新たな病原因子候補2014

    • Author(s)
      西川 明芳
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] In vivo expressed tag immunoprecipitatio 法による感染時の細菌遺伝子発現の網羅的解析2013

    • Author(s)
      堀口安彦
    • Organizer
      第36回分子生物学会
    • Place of Presentation
      神戸市 ポートアイランド
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 気管支敗血症菌が宿主体内で発現する新たな病原因子候補2013

    • Author(s)
      西川明芳, 安倍裕順, 岡田圭祐, 神谷重樹, 福井理, 堀口安彦
    • Organizer
      第66回日本細菌学会関西支部総会
    • Place of Presentation
      大阪大学 微生物病研究所
    • Year and Date
      2013-11-16
    • Related Report
      2013 Final Research Report
  • [Presentation] ブタ由来気管支敗血症菌 S798 株のゲノム解析2013

    • Author(s)
      岡田圭祐, 安倍裕順, 小椋義俊, 林哲也, 阿部章夫, 桑江朝臣, 堀口安彦
    • Organizer
      第66回日本細菌学会関西支部総会
    • Place of Presentation
      大阪大学 微生物病研究所
    • Year and Date
      2013-11-16
    • Related Report
      2013 Final Research Report
  • [Presentation] ブタ由来気管支敗血症菌 S798 株のゲノム解析2013

    • Author(s)
      岡田圭祐, 安倍裕順, 小椋義俊, 林哲也, 阿部章夫, 桑江朝臣, 堀口安彦
    • Organizer
      第7回細菌学若手コロッセウム
    • Place of Presentation
      広島県三原市 広島エアポートホテル
    • Year and Date
      2013-08-08
    • Related Report
      2013 Final Research Report
  • [Presentation] ボルデテラ属細菌が産生するアデニレートサイクラーゼ毒素(ACT)の菌種間における毒素作用の違いについて2013

    • Author(s)
      福井理, 戸嶋ひろ野, 安倍裕順, 神谷重樹, 堀口安彦
    • Organizer
      第60回毒素シンポジウム
    • Place of Presentation
      兵庫県宍粟市 楓香荘
    • Year and Date
      2013-07-18
    • Related Report
      2013 Final Research Report
  • [Presentation] The pore-forming mechanism of Clostridium perfringens enterotoxin2013

    • Author(s)
      Horiguchi Y
    • Organizer
      ETOX16
    • Place of Presentation
      Freiburg Germany Hotel Schloss Reinach
    • Year and Date
      2013-06-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Attempts to understand how the pathogenic bordetellae exert specific pathogenicity in specific hosts2013

    • Author(s)
      Horiguchi Y
    • Organizer
      第155回日本獣医学会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] A putative virulence factor produced by Bordetella2013

    • Author(s)
      Nishikawa S, Abe H, Okada K, Kamitani S, Fukui A, Horiguchi Y
    • Organizer
      第86回日本細菌学会
    • Place of Presentation
      千葉市幕張メッセ
    • Year and Date
      2013-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] IVET-IP Analysis Monitoring Temporal Gene Expression Profiles of Bordetella bronchiseptica in Rats2013

    • Author(s)
      Abe H, Kamitani S, Fukui A, Okada K, Horiguchi Y
    • Organizer
      第86回日本細菌学会
    • Place of Presentation
      千葉市 幕張メッセ
    • Year and Date
      2013-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] In vivo expressed-tag immunoprecipitation 法による感染時の細菌遺伝子発現の網羅的解析2013

    • Author(s)
      堀口 安彦
    • Organizer
      第36回分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] The pore-forming mechanism of Clostridium perfringens enterotoxin2013

    • Author(s)
      堀口 安彦
    • Organizer
      ETOX16
    • Place of Presentation
      Freiburg, Germany
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ボルデテラ属細菌が産生するアデニレートサイクラーゼ毒素 (ACT)の菌種間における毒素作用の違いについて2013

    • Author(s)
      福井 理
    • Organizer
      第60回毒素シンポジウム
    • Place of Presentation
      兵庫
    • Related Report
      2013 Annual Research Report
  • [Presentation] ブタ由来気管支敗血症菌S798株のゲノム解析2013

    • Author(s)
      岡田 圭祐
    • Organizer
      第7回細菌学若手コロッセウム
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] ブタ由来気管支敗血症菌S798株のゲノム解析2013

    • Author(s)
      岡田 圭祐
    • Organizer
      第66回日本細菌学会関西支部総会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] 気管支敗血症菌が宿主体内で発現する新たな病原因子候補2013

    • Author(s)
      西川 明芳
    • Organizer
      第66回日本細菌学会関西支部総会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] 気管支敗血症菌が宿主体内で発現する新たな病原因子候補2013

    • Author(s)
      西川 明芳
    • Organizer
      第36回分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] ブタ由来気管支敗血症菌S798株のゲノム解析2013

    • Author(s)
      岡田 圭祐
    • Organizer
      第36回分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] IVET-IP Analysis Monitoring Temporal Gene Expression Profiles of Bordetella bronchiseptica in Rats2013

    • Author(s)
      H. Abe
    • Organizer
      第86回日本細菌学会
    • Place of Presentation
      横浜市幕張メッセ
    • Related Report
      2012 Annual Research Report
  • [Presentation] A putative virulence factor produced by Bordetella2013

    • Author(s)
      S. Nishikawa
    • Organizer
      第86回日本細菌学会
    • Place of Presentation
      横浜市幕張メッセ
    • Related Report
      2012 Annual Research Report
  • [Presentation] Attempts to understand how the pathogenic bordetellae exert specific pathogenicityi in specific hosts2013

    • Author(s)
      Y. Horiguchi
    • Organizer
      第155回日本獣医学会
    • Place of Presentation
      東京大学駒場キャンパス
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 「なぜ百日咳菌はヒトだけに感染して激しい咳発作を起こすのか?」その基礎細菌学的アプローチ2012

    • Author(s)
      堀口安彦
    • Organizer
      第39回地方衛生研究所全国協議会近畿支部細菌部会研究会
    • Place of Presentation
      大阪市 天王寺区役所
    • Year and Date
      2012-11-09
    • Related Report
      2013 Final Research Report
  • [Presentation] A functional difference in adenylate cyclase toxins produced by Bordetella pertussis and Bordetella bronchiseptica2012

    • Author(s)
      Horiguchi Y
    • Organizer
      IEIIS 2012 Homeostatic Inflammation Symposium
    • Place of Presentation
      National Center of Sciences Building, Tokyo
    • Year and Date
      2012-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] ボルデテラ属細菌の感染特異性決定機構解析へのアプローチ2012

    • Author(s)
      堀口安彦
    • Organizer
      第79回日本細菌学会北海道支部学術集会
    • Place of Presentation
      北海道帯広市 とかちプラザ
    • Year and Date
      2012-08-28
    • Related Report
      2013 Final Research Report
  • [Presentation] The molecular action of Clostridium perfringens enterotoxin, a pore-forming toxin2012

    • Author(s)
      Horiguchi Y
    • Organizer
      日本細菌学会
    • Place of Presentation
      長崎市 長崎ブリックホール
    • Year and Date
      2012-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Different activities of adenylate cyclase toxins of Bordetella pertussis and B.bronchiseptica2012

    • Author(s)
      戸嶋ひろ野, 福井理, 安倍裕順, 神谷重樹, 堀口安彦
    • Organizer
      日本細菌学会
    • Place of Presentation
      長崎市 長崎ブリックホール
    • Year and Date
      2012-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] A functional difference in adenylate cyclase toxins produced by Bordetella pertussis and Bordetellabronchiseptica2012

    • Author(s)
      Y. Horiguchi
    • Organizer
      IEIIS 2012 Homeostatic Inflammation Symposium
    • Place of Presentation
      National Center of Sciences Building, Tokyo
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ボルデテラ属細菌の感染特異性決定機構解析へのアプローチ2012

    • Author(s)
      堀口安彦
    • Organizer
      第79回日本細菌学会北海道支部学術集会
    • Place of Presentation
      北海道帯広市 とかちプラザ
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 「なぜ百日咳菌はヒトだけに感染して激しい咳発作を起こすのか?」 その基礎細菌学的アプローチ2012

    • Author(s)
      堀口安彦
    • Organizer
      第39回地方衛生研究所全国協議会近畿支部細菌部会研究会
    • Place of Presentation
      大阪市天王寺区役所
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Different activities of adenylate cyclase toxins of Bordetella pertussis and B.bronchiseptica2012

    • Author(s)
      戸嶋ひろ野
    • Organizer
      第85回日本細菌学会
    • Place of Presentation
      長崎市長崎ブリックホール
    • Related Report
      2011 Annual Research Report
  • [Presentation] The molecular action of Clostridium perfringens enterotoxin,a pore-forming toxin2012

    • Author(s)
      Y. Horiguchi
    • Organizer
      第85回日本細菌学会
    • Place of Presentation
      長崎市長崎ブリックホール
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順, 神谷重樹, 福井理, 戸嶋ひろ野, 堀口安彦
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜市 パシフィコ横浜
    • Year and Date
      2011-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順, 神谷重樹, 福井理, 戸嶋ひろ野, 堀口安彦
    • Organizer
      日本細菌学会関西支部総会
    • Place of Presentation
      大阪市 大阪府立大学中百舌鳥キャンパス
    • Year and Date
      2011-11-19
    • Related Report
      2013 Final Research Report
  • [Presentation] The pore-forming mechanism of clostridium perfringens enterotoxin targeting claudins, components of the tight junction2011

    • Author(s)
      Horiguchi Y, Kitadokoro K, Nishimura K, Kamitani S, Abe H
    • Organizer
      日本生化学会
    • Place of Presentation
      京都市 京都国際会議場
    • Year and Date
      2011-09-23
    • Related Report
      2013 Final Research Report
  • [Presentation] The pore-forming mechanism of clostridium perfringens enterotoxin targeting claudins, components of the tight junction2011

    • Author(s)
      Horiguchi Y, Kitadokoro K, Nishimura K , Kamitani S, Abe H
    • Organizer
      International Union of Microbiological Societies 2011
    • Place of Presentation
      札幌市 札幌コンベンションセンター
    • Year and Date
      2011-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜市パシフィコ横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順
    • Organizer
      第64回日本細菌学会関西支部総会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス
    • Related Report
      2011 Annual Research Report
  • [Presentation] The pore-forming mechanism of clostridium perfringens enterotoxin targeting claudins,components of the tight junction2011

    • Author(s)
      Y. Horiguchi
    • Organizer
      第84回日本生化学会
    • Place of Presentation
      京都市京都国際会議場
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] The pore-forming mechanism of clostridium perfringens enterotoxin targeting claudins,components of the tight junction2011

    • Author(s)
      Y. Horiguchi
    • Organizer
      International Union of Microbiological Societies 2011
    • Place of Presentation
      札幌市札幌コンベンションセンター
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Book] Swine Atrophic Rhinitis Caused by Pasteurella multocida Toxin and Bordetella Dermonecrotic Toxin, Curr Top Microbiol Immunol2012

    • Author(s)
      Horiguchi Y
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report

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Published: 2013-05-15   Modified: 2023-03-16  

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