• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The bacterial effector-mediated inhibition of caspase-1 activation

Research Project

Project/Area Number 23390106
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionUniversity of the Ryukyus

Principal Investigator

SUZUKI Toshihiko  琉球大学, 医学(系)研究科(研究院), 教授 (10292848)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywordsインフラマゾーム / カスパーゼ-1 / 腸炎ビブリオ / エフェクター / 免疫回避 / マクロファージ / 炎症
Research Abstract

In this work we found that both the NLRP3 and NLRC4 inflammasomes are activated by thermostable direct hemolysins (TDHs) and type III secretion system 1 (T3SS1) in response to V. parahaemolyticus infection. The activated inflammasomes then triggers the activation of caspase-1, a cysteine protease that is essential for IL-1beta processing and release. However, the bacteria also have a function to suppress inflammasome activation via T3SS1. We identified T3SS1 secreted effector proteins, VopQ and VopS, prevent mainly NLRC4 inflammasome activation. VopQ and VopS induce autophagy and the inactivation of Rho GTPases, including Cdc42, respectively, and these cellular events interfere with the assembly of specks, the platform of inflammasome activation. Collectively, T3SS1 effector-based suppression of inflammasome activation may provide important insights into bacterial strategies for evading inflammasome-mediated host immune responses.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (43 results)

All 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 1 results) Presentation (23 results) (of which Invited: 6 results) Book (8 results) Remarks (3 results)

  • [Journal Article] Shigella type III secretion protein MxiI is recognized by Naip2 to induce Nlrc4 inflammasome activation independently of Pkc δ.2014

    • Author(s)
      Suzuki S, Franchi L, He Y, Muioz-Planillo R, Mimuro H, Suzuki T, Sasakawa C, Nunez G.
    • Journal Title

      PLoS Pathog.

      Volume: 10 Issue: 2 Pages: e1003926-e1003926

    • DOI

      10.1371/journal.ppat.1003926

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High temperature protein G (HtpG) is an essential virulence factor of Leptospira interrogans2014

    • Author(s)
      King AM, Pretre G, Bartpho T, Sermswam RW, Toma C, Suzuki T, Eshghi A, Picardeau M, Adler B, Murray GL
    • Journal Title

      Infect Immun

      Volume: 82 Issue: 3 Pages: 1123-1131

    • DOI

      10.1128/iai.01546-13

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages.2014

    • Author(s)
      Toma C, Murray GL, Nohara T, Mizuyama M, Koizumi N, Adler B, Suzuki T.
    • Journal Title

      Cell Microbiol

      Volume: 印刷中

    • NAID

      120006941158

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vibrio parahaemolyticus effector proteins suppress inflammasome activation by interfering with host autophagy signaling2013

    • Author(s)
      Naomi Higa, Claudia Toma, Yukiko Koizumi, Noboru Nakasone, Toshitsugu Nohara and Toshihiko Suzuki
    • Journal Title

      PloS Pathogen

      Volume: 9(1) Issue: 1 Pages: e1003142-e1003142

    • DOI

      10.1371/journal.ppat.1003142

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lose the battle to win the war : bacterial strategies for evading host inflammasome activation2013

    • Author(s)
      Higa N, Toma C, Nohara T, Nakasone N, Takaesu G, Suzuki T
    • Journal Title

      Trends Microbiol

      Volume: 21 Issue: 7 Pages: 342-349

    • DOI

      10.1016/j.tim.2013.04.005

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inflammasome activation via intracellular NLRs triggered by bacterial infection2012

    • Author(s)
      Yukiko Koizumi, et al
    • Journal Title

      Cellular microbiology

      Volume: 14(2) Issue: 2 Pages: 149-154

    • DOI

      10.1111/j.1462-5822.2011.01707.x

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics features of intracellular pathogenic Leptospira in infected murine macrophages2011

    • Author(s)
      Toma C, et al
    • Journal Title

      Cell.Microbiol.

      Volume: 13 Issue: 11 Pages: 1783-1792

    • DOI

      10.1111/j.1462-5822.2011.01660.x

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detergents enhance EspB secretion from Escherichia coli strains harboring the locus for enterocyte effacement (LEE) gene2011

    • Author(s)
      Nakasone N, Toma C, Higa N, Koizumi Y, Ogura Y, Suzuki
    • Journal Title

      FEMS Microbiol.Lett.

      Volume: 315 Issue: 2 Pages: 109-114

    • DOI

      10.1111/j.1574-6968.2010.02176.x

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages

    • Author(s)
      Toma C, Murray GL, Nohara T, Mizuyama M, Koizumi N, Adler B, Suzuki T
    • Journal Title

      Cell Microbiol

      Volume: (印刷中)

    • NAID

      120006941158

    • URL

      http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-5822

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Naip2は赤痢菌MxiIを認識しNlrc4 inflammasomeを活性化する2014

    • Author(s)
      鈴木志穂, Luigi Franchi, Yuan He, Raul Munoz-Planillo, 三室仁美, 鈴木敏彦
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 沖縄近海からとれた海洋産物による細菌のIII型分泌機構阻害2014

    • Author(s)
      仲宗根昇, 比嘉直美, Claudia Toma, 高江洲義一, 野原敏次, 鈴木敏彦
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 病原体感染におけるインフラマゾーム活性化2013

    • Author(s)
      鈴木敏彦
    • Organizer
      沖縄感染免疫シンポジウム
    • Place of Presentation
      沖縄
    • Year and Date
      2013-07-31
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Invited
  • [Presentation] インフラマゾーム活性化と慢性炎症疾患との接点2013

    • Author(s)
      鈴木敏彦
    • Organizer
      第3回糖尿病治療の新時代~基礎と臨床を学ぶ~
    • Place of Presentation
      大阪
    • Year and Date
      2013-07-05
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Analysis of the bacterial factors involved in Leptospira interrogans -macrophage interactions2013

    • Author(s)
      Claudia Toma, Gerald L. Murray, Naomi Higa, Noboru Nakasone, Toshitsugu Nohara, Giichi Takaesu, Nobuo Koizumi, Ben Adler, Toshihiko Suzuki
    • Organizer
      8th Scientific Meeting of International Leptospirosis Society
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] High temperature protein G (HtpG) is essential for acute leptospirosis in hamsters2013

    • Author(s)
      Amy M. King, Gabriela Pretre, Thanatchaporn Bartpho, Rasana W. Sermswan, Claudia Toma, Toshihiko Suzuki, Mathieu Picardeau, Ben Adler, Gerald L.Murray
    • Organizer
      8th Scientific Meeting of International Leptospirosis Society
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 細菌のIII型分泌機構を阻害する沖縄県海域に生息する海産物の調査2013

    • Author(s)
      仲宗根昇, 比嘉直美, トーマ・クラウディア, 高江洲義一, 野原敏次, 鈴木敏彦
    • Organizer
      第66回日本細菌学会九州支部総会
    • Related Report
      2013 Final Research Report
  • [Presentation] 細菌感染とNod-like receptor2013

    • Author(s)
      鈴木敏彦
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉
    • Related Report
      2013 Final Research Report
  • [Presentation] Leptospira interrogansとマクロファージの相互作用に関与している病原因子の解析2013

    • Author(s)
      クラウディア・トーマ, 比嘉直美, 仲宗根昇, 野原敏次, 小泉信夫, 鈴木敏彦
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉
    • Related Report
      2013 Final Research Report
  • [Presentation] 下痢原性大腸菌の3型分泌装置を阻害する植物抽出物の発見2013

    • Author(s)
      仲宗根昇, 比嘉直美, 高江洲義一, 野原敏次, トーマ・クラウディア, 鈴木敏彦
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉
    • Related Report
      2013 Final Research Report
  • [Presentation] インフラマゾーム活性化と慢性炎症疾患との接点2013

    • Author(s)
      鈴木敏彦
    • Organizer
      第3回糖尿病治療の新時代~基礎と臨床を学ぶ~
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 細菌のIII型分泌機構を阻害する沖縄県海域に生息する海産物の調査2013

    • Author(s)
      仲宗根昇、比嘉直美、トーマ・クラウディア、高江洲義一、野原敏次、鈴木敏彦
    • Organizer
      第66回日本細菌学会九州支部総会
    • Place of Presentation
      長崎
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌感染とNod-like receptor2013

    • Author(s)
      鈴木敏彦
    • Organizer
      日本細菌学会
    • Place of Presentation
      幕張メッセ(千葉)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Leptospira interrogansとマクロファージの相互作用に関与している病原因子の解析2013

    • Author(s)
      クラウディア・トーマ、比嘉直美、仲宗根昇、野原敏次、小泉信夫、鈴木敏彦
    • Organizer
      日本細菌学会
    • Place of Presentation
      幕張メッセ(千葉)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 下痢原性大腸菌の3型分泌装置を阻害する植物抽出物の発見2013

    • Author(s)
      仲宗根昇、比嘉直美、高江洲義一、野原敏次、トーマ・クラウディア、鈴木敏彦
    • Organizer
      日本細菌学会
    • Place of Presentation
      幕張メッセ(千葉)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Caspase-1-mediated host immune responses triggered by inflammasome upon bacterial infecion2012

    • Author(s)
      Suzuki T
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      ブリックホール(長崎市)(招待講演)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effector proteins secreted via T3SS1 of Vibrio parahaemolyticus interfere with inflammasome activation2012

    • Author(s)
      比嘉直美, クラウディア・トーマ, 仲宗根昇, 鈴木敏彦
    • Organizer
      第42回日本免疫学会学術総会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] NLR活性化と病原体感染とのかかわり2012

    • Author(s)
      鈴木敏彦
    • Organizer
      第40回日本臨床免疫学会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Caspase-1-mediated host immune responses triggered by inflammasome upon bacterial infecion2012

    • Author(s)
      Suzuki T
    • Organizer
      第85回日本細菌学会総会国際シンポジウム
    • Place of Presentation
      長崎
    • Related Report
      2013 Final Research Report
  • [Presentation] NLR活性化と病原体感染とのかかわり2012

    • Author(s)
      鈴木敏彦
    • Organizer
      日本臨床免疫学会
    • Place of Presentation
      京王プラザホテル(東京)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Effector proteins secreted via T3SS1 of Vibrio parahaemolyticus interfere with inflammasome activation2012

    • Author(s)
      比嘉直美、クラウディア・トーマ、仲宗根昇、鈴木敏彦
    • Organizer
      日本免疫学会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Bacterial infection and inflammasome activaiton mediated by NOD-like receptors2011

    • Author(s)
      Suzuki T
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター(札幌市)(招待講演)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Bacterial infection and inflammasome activation mediated by NOD-like receptors2011

    • Author(s)
      Suzuki T
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Book] 標準微生物学2014

    • Author(s)
      中込治, 神谷茂編集(鈴木敏彦, 他執筆)
    • Publisher
      医学書院(印刷中)
    • Related Report
      2013 Final Research Report
  • [Book] 標準微生物学2014

    • Author(s)
      中込治、神谷茂編集( 鈴木敏彦、他執筆)
    • Publisher
      医学書院
    • Related Report
      2013 Annual Research Report
  • [Book] 実験医学 7月号特集「インフラマソーム」2012

    • Author(s)
      鈴木敏彦
    • Publisher
      羊土社
    • Related Report
      2013 Final Research Report
  • [Book] 細菌感染による NLRP3 および NLRC4 を介したインフラマソーム活性化, 感染・炎症・免疫2012

    • Author(s)
      小泉由起子, 鈴木敏彦
    • Publisher
      医学の門社
    • Related Report
      2013 Final Research Report
  • [Book] 実験医学7月号特集「インフラマゾーム」2012

    • Author(s)
      鈴木敏彦
    • Total Pages
      6
    • Publisher
      羊土社
    • Related Report
      2012 Annual Research Report
  • [Book] 細菌感染によるNLRP3およびNLRC4を介したインフラマソーム活性化(雑誌「感染・炎症・免疫)2012

    • Author(s)
      小泉由起子, 他
    • Publisher
      医薬の門社
    • Related Report
      2011 Annual Research Report
  • [Book] 細菌感染による NLRP3 および NLRC4を介したインフラマソーム活性化, 生体の科学2011

    • Author(s)
      小泉由起子, 比嘉直美, 鈴木敏彦
    • Publisher
      医学書院
    • Related Report
      2013 Final Research Report
  • [Book] 細菌感染によるNLRP3およびNLRC4を介したインフラマソーム活性化(雑誌「生体の科学」)2011

    • Author(s)
      小泉由起子, 他
    • Publisher
      医学書院
    • Related Report
      2011 Annual Research Report
  • [Remarks] 琉球大学大学院医学研究科細菌学講座

    • URL

      http://w3.u-ryukyu.ac.jp/bacteriology

    • Related Report
      2013 Annual Research Report
  • [Remarks] 琉球大学大学院医学研究科細菌学講座

    • URL

      http://w3.u-ryukyu.ac.jp/bacteriology

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://w3.u-ryukyu.ac.jp/bacteriology

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi