| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
|
|---|
| Outline of Final Research Achievements |
The host range of enterovirus 71 (EV71) is restricted to primates with some exceptions. Therefore it was difficult to evalate the neurovirulence level of EV71 strains. We have established a transgenic mouse model that expressed a cellular receptor for EV71, human Scavenger receptor B2 (SCARB2). The transgenic mice became susceptible to EV71. They showed efficient EV71 replication in neurons in the CNS and neurological signs similar to those observed in human severe cases infected with EV71.
We inoculated several EV71 strains and compared the virulence of these strains. The inoculated virus exhibited different virulence levels, suggesting that the tg mice are useful model. We then evaluated the effect of a single amino acid substitution Gly to Glu at position 145 of the VP1 capsid protein. The virus with Glu at this position significantly increased the virulence compared to that with Gly. The result suggested that this amino acid is an important virulence determinant in EV71.
|
