Molecular basis of enterovirus 71 neuropathogenicity
Project/Area Number |
23390116
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Virology
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Research Institution | Tokyo Metropolitan Institute of Medical Science |
Principal Investigator |
KOIKE Satoshi 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 副参事研究員 (30195630)
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Co-Investigator(Kenkyū-buntansha) |
FUJII Ken 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (10580201)
YAMAYOSHI Seiya 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (50529534)
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Co-Investigator(Renkei-kenkyūsha) |
NAGATA Noriyo 国立感染症研究所, 感染病理部, 室長 (30270648)
SHIMIZU Hiroyuki 国立感染症研究所, ウイルス第二部, 室長 (90270644)
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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Keywords | エンテロウイルス71 / 神経病原性 / マウス感染モデル / 感染症 / ウイルス / ウイルス受容体 / 神経毒力 / 脳神経疾患 |
Outline of Final Research Achievements |
The host range of enterovirus 71 (EV71) is restricted to primates with some exceptions. Therefore it was difficult to evalate the neurovirulence level of EV71 strains. We have established a transgenic mouse model that expressed a cellular receptor for EV71, human Scavenger receptor B2 (SCARB2). The transgenic mice became susceptible to EV71. They showed efficient EV71 replication in neurons in the CNS and neurological signs similar to those observed in human severe cases infected with EV71. We inoculated several EV71 strains and compared the virulence of these strains. The inoculated virus exhibited different virulence levels, suggesting that the tg mice are useful model. We then evaluated the effect of a single amino acid substitution Gly to Glu at position 145 of the VP1 capsid protein. The virus with Glu at this position significantly increased the virulence compared to that with Gly. The result suggested that this amino acid is an important virulence determinant in EV71.
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Report
(5 results)
Research Products
(34 results)
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[Journal Article] Establishment of a panel of in-house polyclonal antibodies for the diagnosis of enterovirus infections2014
Author(s)
Kotani O, Iwata-Yoshikawa N, Suzuki T, Sato Y, Nakajima N, Koike S, Iwasaki T, Sata T, Yamashita T, Minagawa H, Taguchi F, Hasegawa H, Shimizu H, Nagata N
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Journal Title
Neuropathology
Volume: 35
Issue: 2
Pages: 107-121
DOI
Related Report
Peer Reviewed
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[Journal Article] Human SCARB2-dependent Infection by Coxsackievirus A7, A14, A16 and Enterovirus 712012
Author(s)
Yamayoshi S, Iizuka S, Yamahsita T, Muzuta K, Okamoto M, Nishimura H, Sanjoh S, Katsushima N, Itagaki T, Nagai Y, Fujii K, Koike S
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Journal Title
Journal of Virology
Volume: 86
Pages: 5686-5696
Related Report
Peer Reviewed
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[Journal Article] The association of recombination events in the founding and emergence of subgenogroup evolutionary lineages of human enterovirus 712012
Author(s)
McWilliam Leitch EC, Cabrerizo M, Cardosa J, Harvala H, Ivanova OE, Koike S, Kroes AC, Lukashev A, Perera D, Roivainen M, Susi P, Trallero G, Evans DJ, Simmonds P
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Journal Title
Journal of Virology
Volume: 86
Issue: 5
Pages: 2676-2685
DOI
Related Report
Peer Reviewed
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[Presentation] Human SCARB2-dependent infection of clinical isolates of coxsackievirus A14, A16 and Enterovirus 712011
Author(s)
Yamayoshi S, Iizuka S, Yamashita T, Minagawa H, Sanjoh K, Kasushima N, Itagaki, T, Mizuta K, Nagai Y, Okamoto M, Nishimura H, Fujii K, Koike S
Organizer
The XVth International Congress of Virology
Place of Presentation
札幌コンベンションセンター(札幌市)
Year and Date
2011-09-16
Related Report
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