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Molecular basis for Epstein-Barr virus replication

Research Project

Project/Area Number 23390118
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionAichi Cancer Center Research Institute

Principal Investigator

TSURUMI Tatsuya  愛知県がんセンター(研究所), 腫瘍ウイルス学部, 部長 (90172072)

Co-Investigator(Renkei-kenkyūsha) KANDA Teru  愛知県がんセンター(研究所), 腫瘍ウイルス学部, 室長 (50333472)
MURATA Takayuki  愛知県がんセンター(研究所), 腫瘍ウイルス学部, 研究員 (30470165)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
KeywordsEBウイルス / 複製 / 転写 / 増殖 / EBウイルス / エピジェネチックス / EBV / ウイルス / カプシド
Research Abstract

Epstein-Barr virus possesses two alternative life cycles, latent and lytic.
In the latent phase, reactivation of BZLF1 promoter is inhibited. We determined that histons around the promoter region we re negatively methylated and positive histon markers were increased after induction of lytic replication. JDP2 transcription factor contributed the establishment of the latent infection. In the lytic phase, viral replication occurs in replication compartments(RC). BMRF-1 core exists within RC. Viral DNA packaging proteins were found in the core and viral immediate-eartly and early transcription occurs outside the core, while late transcription was inside. Thus, each events were compartmentalized by the BMRF-1 core.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (45 results)

All 2013 2012 2011 Other

All Journal Article (26 results) (of which Peer Reviewed: 26 results) Presentation (19 results) (of which Invited: 2 results)

  • [Journal Article] Pin1 interacts with the Epstein-Barr virus DNA polymerase catalytic subunit and regulates viral DNA replication2013

    • Author(s)
      Narita Y, Murata T, Ryo A, Kawashima D, Sugimoto A, Kanda T, Kimura H, Tsurumi T
    • Journal Title

      J Virol

      Volume: 87 Pages: 2120-2127

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome guardian p53 and viral infections2013

    • Author(s)
      Sato Y, Tsurumi T
    • Journal Title

      Rev Med Virol

      Volume: 23 Pages: 213-220

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epstein-Barr virus deubiquitinase downregulates TRAF6-mediated NF-κB signaling during productive replication2013

    • Author(s)
      Saito S, Murata T, Kanda T, Isomura H, Narita Y, Sugimoto A, Kawashima D, Tsurumi T
    • Journal Title

      J Virol

      Volume: 87 Pages: 4060-4070

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear Transport of Epstein-Barr Virus DNA Polymerase is dependent on the BM RF1 Polymerase Processivity Factor and M olecular Chaperone Hsp902013

    • Author(s)
      Kawashim a D, Kanda T, Murata T, Saito S, Sugimoto A, Narita Y, Tsurumi T
    • Journal Title

      J Virol

      Volume: 87 Pages: 6482-6491

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Different Distributions of Epstein-Barr Virus Early and Late Gene Transcripts within Viral Replication Com partm ents2013

    • Author(s)
      Sugim to A, Sato Y, Kanda T, Mrata T, Narita Y, Kawashim D, Kimra H, Tsurumi T
    • Journal Title

      J Virol

      Volume: 87 Pages: 6693-6699

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic modification of the Epstein-Barr virus BZLF1 promoter regulates viral reactivation from latency2013

    • Author(s)
      Murata T, Tsurumi T
    • Journal Title

      Front Genet

      Volume: 4 Pages: 53-53

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heat Shock Protein 90 Inhibitors Repress Latent Mem brane Protein 1 (LM P1) Expression and Proliferation of Epstein-Barr virus-Positive Natural Killer Cell Lym phoma2013

    • Author(s)
      Murata T, Iwata S, Alam Siddiquey M, Kanazawa T, Goshima F, Kimura H, Tsurumi T
    • Journal Title

      PLoS One

      Volume: 8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction between Basic Residues of Epstein-Barr Virus EBNA1 Protein and Cellular Chrom atin M ediates Viral Plasm id M aintenance2013

    • Author(s)
      Kanda T, Horikoshi N, Murata T, Kawashima D, Sugimoto A, Narita Y, Kurumizaka H, Tsurumi T
    • Journal Title

      J Biol Chem

      Volume: 288 Pages: 24189-24199

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of M yocyte Enhancer Factor 2 (M EF2) Fam ily Transcription Factors to BZLF1 Expression in Epstein-Barrvirus Reactivation from Latency2013

    • Author(s)
      Murata T, Narita Y, Sugimoto A, Kawashima D, Kanda T, Tsurumi T
    • Journal Title

      J Virol

      Volume: 87 Pages: 10148-10162

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of Myocyte Enhancer Factor 2 (MEF2) Family Transcription Factors to BZLF1 Expression in Epstein-Barr virus Reactivation from Latency.2013

    • Author(s)
      Murata T, Narita Y, Sugimoto A, Kawashima D, Kanda T, Tsurumi T
    • Journal Title

      Journal of Virology

      Volume: 87 Issue: 18 Pages: 10148-10162

    • DOI

      10.1128/jvi.01002-13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic modification of the Epstein-Barr virus BZLF1 promoter regulates viral reactivation from latency.2013

    • Author(s)
      Murata T, Tsurumi T.
    • Journal Title

      Front Genet

      Volume: 4 Pages: 53-53

    • DOI

      10.3389/fgene.2013.00053

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Different Distributions of Epstein-Barr Virus Early and Late Gene Tran scripts within Viral Replication Compartments2013

    • Author(s)
      Atsuko Sugimoto, Yoshitaka Sato, Teru Kanda, Takayuki Murata, Yohei Narita, Daisuke Kawashima, Hiroshi Kimura, Tatsuya Tsurumi
    • Journal Title

      Journal of Virology

      Volume: (印刷中(In press) Issue: 12 Pages: 6693-6699

    • DOI

      10.1128/jvi.00219-13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear transport of Epstein-Barr virus DNA polymerase is dependent on the BMRF1 polymerase processivity factor and molecular chaperone Hsp90.2013

    • Author(s)
      Kawashima D, Kanda T, Murata T, Saito S, Sugimoto A, Narita Y, Tsurumi T.
    • Journal Title

      Journal of Virology

      Volume: 87 Issue: 11 Pages: 6482-6491

    • DOI

      10.1128/jvi.03428-12

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epstein-Barr virus deubiquitinase downregulates TRAF6-mediated NF-κB signaling during productive replication.2013

    • Author(s)
      Saito S, Murata T, Kanda T, Isomura H, Narita Y, Sugimoto A, Kawashima D, Tsurumi T.
    • Journal Title

      Journal of Virology

      Volume: 87 Issue: 7 Pages: 4060-4070

    • DOI

      10.1128/jvi.02020-12

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic Histone M odification of Epstein-Barr Virus BZLF1 Prom oter during Latency and Reactivation in Raji Cells2012

    • Author(s)
      Murata T, Kondo Y, Sugimoto A, Kawashima D, Saito S, Isomura H, Kanda T, Tsurumi T
    • Journal Title

      J Virol

      Volume: 86 Pages: 4752-4761

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of down-regulated neutral ceram idase during all-trans retinoic acid induced neuronal differentiation in SH-SY5Y neuroblastom a cells2012

    • Author(s)
      Tanaka K, Tam iya-Koizum i K, Hagiwara K, Ito H, Takagi A, Kojim a T, Suzuki M, Iwaki S, Fujii S, Nakamura M, Banno Y, Kannagi R, Tsurumi T, Kyogashima M, Murate T
    • Journal Title

      J Biochem

      Volume: 151 Pages: 611-620

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] HLA-restricted presentation of W T1 tum or antigen in B-lym phoblastoid cell lines established using a m axi-EBV system2012

    • Author(s)
      Kanda T, Ochi T, Fujiwara H, Yasukawa M, Okamoto S, Mineno J, Kuzushima K, Tsurumi T
    • Journal Title

      Cancer Gene Ther

      Volume: 19 Pages: 566-571

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells.2012

    • Author(s)
      Murata T.
    • Journal Title

      J Virol.

      Volume: 86(9) Issue: 4 Pages: 4752-61

    • DOI

      10.1128/jvi.02634-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatiotem porally Different DNA Repair System s participate during Epstein-Barr Virus Genom e Maturation2011

    • Author(s)
      Sugim oto A, Kanda T, Yam ashita Y, M urata T, Saito S, Kawashim a D, Isom ura H, Nishiyam a Y, Tsurum i T
    • Journal Title

      J Virol

      Volume: 85 Pages: 6127-6135

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hum an cytom egalovirus gene products essential for late viral gene expression assem ble into pre- replication com plexes before viral DNA replication2011

    • Author(s)
      Isom ura H, Stinski M S, M urata T, Yam ashita Y, Kanda T, Toyokuni S, Tsurumi T
    • Journal Title

      J Virol

      Volume: 85 Pages: 6629-6644

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvem ent of Jun Dim erization Protein 2 (JDP2) in the M aintenance of Epstein-Barr Virus Latency2011

    • Author(s)
      Murata T, Noda C, Saito S, Kawashima D, Sugimoto A, Isomura H, Kanda T, Yokoyama K, Tsurum I T
    • Journal Title

      J Biol Chem

      Volume: 286 Pages: 22007-22016

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of CCAAT enhancer-binding protein (C/EBP) as a transcriptional activator for Epstein-Barr virus oncogene latent m em brane protein 12011

    • Author(s)
      Noda C, M urata T, Kanda T, Yoshiyam a H, Sugim oto A, Kawashim a D, Saito S, Isomura H, Tsurum I T
    • Journal Title

      J Biol Chem

      Volume: 286 Pages: 42524-42533

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unexpected instability of fam ily of repeats (FR), the critical cis-acting sequence required for EBV latent infection, in EBV-BAC systems2011

    • Author(s)
      Kanda T, Shibata S, Saito S, M urata T, Isomura H, Yoshiyama H, Takada K, Tsurumi T
    • Journal Title

      PLoS One

      Volume: 6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of CCAAT enhancer-binding protein (C/EBP) as a transcriptional activator for Epstein-Barr virus oncogene latent membrane protein 12011

    • Author(s)
      Noda C, et.al
    • Journal Title

      J.Biol.Chem.

      Volume: 286(49) Issue: 49 Pages: 42524-42533

    • DOI

      10.1074/jbc.m111.271734

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Jun dimerization protein 2 (JDP2) in the maintenance of Epstein-Barr virus latency2011

    • Author(s)
      Murata T, et.al
    • Journal Title

      J.Biol.Chem.

      Volume: 286(25) Issue: 25 Pages: 22007-22016

    • DOI

      10.1074/jbc.m110.199836

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatiotemporally different DNA repair systems participate in Epstein-Barr virus genome maturation2011

    • Author(s)
      Sugimoto A, et.al
    • Journal Title

      J.Virol.

      Volume: 85(13) Issue: 13 Pages: 6127-6135

    • DOI

      10.1128/jvi.00258-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Epstein-Barr virus Genome Packaging Factors converge in inner Genom e Storeroom s of BM RF1 cores within Viral Replication Compartments2013

    • Author(s)
      Tsurumi T
    • Organizer
      SGM spring meeting
    • Place of Presentation
      M anchester, UK
    • Related Report
      2013 Final Research Report
  • [Presentation] Nuclear Transport of Epstein-Barr Virus DNA Polym erase is dependent on the BM RF1 Polym erase Processivity Factor and Molecular Chaperone Hsp902013

    • Author(s)
      Tsurumi T, Kawashima D
    • Organizer
      The Biology of M olecular Chaperones : From molecules, organelles and cells to m isfolding diseases
    • Place of Presentation
      Santa Margheritadi Pula, Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] Roles of BART m icroRNAs in EBV-infected epithelial cells2013

    • Author(s)
      Kanda T, Murata T, Tsurumi T
    • Organizer
      6th International Symposium on Nasopharyngeal Carcinoma
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2013 Final Research Report
  • [Presentation] Induction of EBV Oncogene LMP1 by AP-2 in NPC Cells.2013

    • Author(s)
      Murata T, Noda C, Kanda T, Tsurumi T
    • Organizer
      6th International Symposium on Nasopharyngeal Carcinoma
    • Place of Presentation
      stanbul, Turkey
    • Related Report
      2013 Final Research Report
  • [Presentation] Induction of EBV Oncogene LMP1 by AP-2 in NPC Cells.2013

    • Author(s)
      Murata T, Noda C, Kanda T, Tsurumi T
    • Organizer
      6th International Symposium on Nasopharyngeal Carcinoma
    • Place of Presentation
      Istanbul
    • Related Report
      2013 Annual Research Report
  • [Presentation] Roles of BART microRNAs in EBV-infected epithelial cells.2013

    • Author(s)
      Kanda T, Murata T, Tsurumi T
    • Organizer
      6th International Symposium on Nasopharyngeal Carcinoma
    • Place of Presentation
      Istanbul
    • Related Report
      2013 Annual Research Report
  • [Presentation] Nuclear Transport of Epstein-Barr Virus DNA Polymerase is dependent on the BMRF1 Polymerase Processivity Factor and Molecular Chaperone Hsp90.2013

    • Author(s)
      Tsurumi T, Kawashima D.
    • Organizer
      The Biology of Molecular Chaperones: From molecules, organelles and cells to misfolding diseases
    • Place of Presentation
      Santa Margherita di Pula, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] The Epstein-Barr virus DNA Polymerase Catalytic Subunit BALF5 Interacts with Pin1 for Efficient Productive Viral DNA Replication.2013

    • Author(s)
      Narita Y, Murata T, Kimura H, Tsurumi T.
    • Organizer
      38th Annual International Herpesvirus Workshop
    • Place of Presentation
      Grand Rapids, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Spatiotemporally different DNA repair systems, HRR and MMR, participate in Epstein-Barr virus genome maturation2012

    • Author(s)
      Tsurumi T, et al
    • Organizer
      Maintenance of Genome Stability 2012
    • Place of Presentation
      Sheraton Nassau, Nassau, Bahamas
    • Year and Date
      2012-03-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cis- and trans-elements that affect reactivation of Epstein-Barrvirus from latency2012

    • Author(s)
      Murata T, Tsurumi T
    • Organizer
      International Congress on Oncogenic Herpesviruses and Associated Diseases
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Epstein-Barr virus genome packaging factors converge at inner part of viral genom e storeroom of the BM RF1 core within viral replication compartment2012

    • Author(s)
      Sugimoto A, Kimura H, Tsurumi T
    • Organizer
      International Congress on Oncogenic Herpesviruses and Associated Diseases
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Chrom osom e binding of Epstein-Barr virus EBNA1 protein is m ediated by arginine residues within chrom osom e binding domains2012

    • Author(s)
      Kanda T, Murata T, Tsurumi T
    • Organizer
      International Congress on O ncogenic Herpesviruses and Associated Diseases
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Involvement of Hsp90 in Epstein-Barr Virus Lytic Replication2012

    • Author(s)
      Kawashima D, Tsurumi T
    • Organizer
      The 11th International Congress of Hypertherm ic O ncology (ICHO ) & The 29th Japanese Congress of Therm al M edicine (JCTM )
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] Epstein-Barr virus Genom e Packaging Factors converge in inner Genom e Storeroom s of BM RF1 cores within Viral Replication Com partm ents2012

    • Author(s)
      Tsurumi T, Sugimoto A
    • Organizer
      The 4th EMBO meeting
    • Place of Presentation
      Nice, France
    • Related Report
      2013 Final Research Report
  • [Presentation] Epstein-Barr virus Replication Factory2012

    • Author(s)
      Tsurumi T
    • Organizer
      The 11th Awaji International Forum on Infection and Immnity
    • Place of Presentation
      Awaji
    • Related Report
      2013 Final Research Report
  • [Presentation] Epstein-Barr virus Genome Packaging Factors converge in inner Genome Storerooms of BMRF1 cores within Viral Replication Compartments

    • Author(s)
      Tsurumi T
    • Organizer
      The 4th EMBO meeting
    • Place of Presentation
      Convention Center (Nice)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Epstein-Barr virus Replication Factory

    • Author(s)
      Tsurumi T
    • Organizer
      The 11th Awaji International Forum on Infection and Immnity
    • Place of Presentation
      国際会議場(淡路)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] EBV陽性細胞の増殖を制御するウイルス遺伝子の発現調節機構

    • Author(s)
      鶴見達也
    • Organizer
      感染と癌 -感染癌のエフェクター分子とその標的-
    • Place of Presentation
      北海道大学学友会館(札幌)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Epstein-Barr virus Genome Packaging Factors converge in inner Genome Storerooms of BMRF1 cores within Viral Replication Compartments

    • Author(s)
      Tsurumi T
    • Organizer
      SGM spring meeting
    • Place of Presentation
      Convention Center (Manchester)
    • Related Report
      2012 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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