Study on pathophysiological roles of brain NO-NCX signal
| Project/Area Number |
23390139
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | Osaka University |
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Principal Investigator |
MATSUDA Toshio 大阪大学, 薬学研究科(研究院), 教授 (00107103)
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| Co-Investigator(Kenkyū-buntansha) |
吾郷 由希夫 大阪大学, 薬学研究科, 大阪大学 (50403027)
TAKUMA Kazuhiro 大阪大学, 薬学研究科, 准教授 (90289025)
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| Research Collaborator |
HASHIMOTO Hitoshi 大阪大学, 薬学研究科, 教授 (30240849)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Keywords | Na+/Ca2+交換系 / 一酸化窒素 / 環境要因 / 一酸化窒素（NO） / 細胞障害 / 精神機能 / Na+/Ca2+交換系(NCX) / Nitric oxide (NO) / SEA0400 / ドパミン |
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| Research Abstract |
This study examined the role of the predominant brain-specific NCX splice variant NCX1.5 in NO-induced cytotoxicity in HEK293 cell expression system. Transfection with a plasmid construct pcDNA3.1/V5-His containing full-length rat NCX1.5 cDNA into HEK293 cells increased prominently the protein level of NCX1.5 tagged with C-terminal V5 and (His)6 epitopes (NCX1.5-V5/His). There was no difference in the cytotoxic effects by sodium nitroprusside and S-nitroso-N-acetylpenicillamine between the control and transfected cells. These results suggest that NO cytotoxicity is not dependent on NCX1.5. Microdialysis study showed that NO did not affect monoamine release in the brain. Furthermore, RT-PCR analysis showed that environmental factors did not affect the expression of NCX.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Pretreatment of donor islets with the Na+/Ca2+ exchanger inhibitor improves the efficiency of islet transplantation2013
Author(s)
Mera T, Itoh T, Kita S, Kodama S, Kojima D, Nishinakamura H, Okamoto K, Ohkura M, Nakai J, Iyoda T, Iwamoto T, Matsuda T, Baba A, Omori K, Ono J, Watarai H, Taniguchi M, Yasunami Y
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Journal Title
Am J Transplant
Volume: 13(8)
Pages: 2154-2160
Related Report
Peer Reviewed
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[Journal Article] Pretreatment of donor islets with the Na(+) /Ca(2+) exchanger inhibitor improves the efficiency of islet transplantation2013
Author(s)
Mera T, Itoh T, Kita S, Kodama S, Kojima D, Nishinakamura H, Okamoto K, Ohkura M, Nakai J, Iyoda T, Iwamoto T, Matsuda T, Baba A, Omori K, Ono J, Watarai H, Taniguchi M, Yasunami Y.
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Journal Title
Am J Transplant
Volume: 13
Issue: 8
Pages: 2154-2160
DOI
Related Report
Peer Reviewed
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[Journal Article] Preferential involvement of Na+/Ca2+ exchanger type-1 in the brain damage caused by transient focal cerebral ischemia in mice2012
Author(s)
Morimoto N, Kita S, Shimazawa M, Namimatsu H, Tsuruma K, Hayakawa K, Mishima K, Egashira N, Iyoda T, Horie I, Gotoh Y, Iwasaki K, Fujiwara M, Matsuda T, Baba A, Komuro I, Horie K, Takeda J, Iwamoto T, Hara H
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Journal Title
Biochem Biophys Res Commun
Volume: 429(3-4)
Pages: 186-190
Related Report
Peer Reviewed
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[Journal Article] SEA0400, a specific Na+/Ca2+ exchange inhibitor, prevents dopaminergic neurotoxicity in an MPTP mouse model of Parkinson's disease2011
Author(s)
Ago Y, Kawasaki T, Nashida T, Ota Y, Cong Y, Kitamoto M, Takahashi T, Takuma K, Matsuda T
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Journal Title
Neuropharmacology
Volume: 61(8)
Pages: 1441-1451
Related Report
Peer Reviewed
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