Development of targeted medical care (simultaneous diagnostic imaging and therapy) for pathogenic angiogenesis using fibrinolytic metabolites

• Project/Area Number: 23390151
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Okayama University
• Principal Investigator: MATSUURA Eiji 岡山大学, 医歯(薬)学総合研究科, 教授 (20181688)
• Project Period (FY): 2011-11-18 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 血管新生 / ベータ2-グリコプロテインI / 抗血管新生剤 / B2GPI-ドメインI / nicked B2GPI / PETイメージング / 質量分析 / DDSキャリア / beta2 glycoprotein I / B2GPI-DI / VEGF / β2GPI / nicked-β2GPI / β2GPI-DI / DOTA修飾 / VEGF受容体 / MALDI-TOF-MS / β2-グリコプロテインI(β2GPI) / β2GPI-DV / nicked DV / 抗血管新生 / マイクロキャリア / 動脈硬化 / Theranostics

Research Abstract

By the cytostatic activity test and angiogenesis imaging analysis with human umbilical vein endothelial cells, we confirmed that DI of beta2-glycoprotein I (B2GPI) revealed dose- and time-dependent cytostatic and anti-angiogenesis activity. 64Cu-labeled DI and 64Cu-labeled B2GPI were prepared and used for PET/CT imaging of tumor-bearing mice. DI showed high tumor/blood ratio. 64Cu-labeled and B2GPI-bound liposomes were prepared as an experimental model of the Theranostics (simultaneous diagnostic imaging and therapy) technology.

Research Products

• [Journal Article] Is atherosclerosis an autoimmune disease? (2014)
  • Author(s): Matsuura E, Atzeni F, Sarzi-Puttini P, Turiel M, Lopez LR, Nurmohamed MT
  • Journal Title: BMC Med Volume: 12 Issue: 1
  • DOI: 10.1186/1741-7015-12-47
  • Peer Reviewed
• [Journal Article] β2-glycoprotein I and oxidative inflammation in early atherogenesis : a progression from innate to adaptive immunity? (2012)
  • Author(s): Matsuura E, Lopez LR, Shoenfeld Y, Ames PR
  • Journal Title: Autoimmun Rev Volume: 12 Issue: 2 Pages: 241-9
  • DOI: 10.1016/j.autrev.2012.04.003
  • Peer Reviewed
• [Journal Article] 7-Ketocholesteryl-9-carboxynonanoate enhances the expression of ATP-binding cassette transporter A1 via CD36 (2012)
  • Author(s): Wenzhe Li
  • Journal Title: Atherosclerosis Volume: 226 Issue: 1 Pages: 102-109
  • DOI: 10.1016/j.atherosclerosis.2012.10.038
  • Peer Reviewed
• [Journal Article] A possible mechanism of autoimmune mediated infertility in women with endometriosis (2011)
  • Author(s): Inagaki J, Hao L, Nakatsuka M, Yasuda T, Hiramatsu Y, Shoenfeld Y, Matsuura E
  • Journal Title: American journal of reproductive immunology Volume: 66 Pages: 90-99
  • Peer Reviewed
• [Journal Article] In vivo oxidation, platelet activation and simultaneous occurrence of natural immunity in atherosclerosis-prone mice (2011)
  • Author(s): Shen L, Matsunami Y, Quan N, Kobayashi K, Matsuura E, Oguma
  • Journal Title: The Israel Medical Association Journal Volume: 13 Pages: 278-283
  • Peer Reviewed
• [Journal Article] Recombinant domain V of β2-glycoprotein I inhibits the formation of a 7-ketocholesteryl-9-carboxynonanoate and β2-glycoprotein I complex (2011)
  • Author(s): Zhang Y, Li W, Chi Y, Wang R, Wang D, Zhang F, Liu Z, Matsuura E, Liu Q
  • Journal Title: Journal of Biochemistry Volume: 149 Pages: 35-42
  • Peer Reviewed
• [Presentation] Autoimmune and infection-mediated atherosclerosis : possible mechanisms and innovation from translational biomedical researches (2014)
  • Author(s): Matsuura E, Lopez L
  • Organizer: 9th International Congress on Autoimmunity
  • Place of Presentation: Nice, France
• [Presentation] Oxidized lipids composed of atherogenic complex autoantigen : by lipidome analyses with cutting-edge imaging mass microscope (iMScope) (2014)
  • Author(s): Shen L, Matsuura E, et al(他6名)
  • Organizer: 9th International Congress on Autoimmunity
  • Place of Presentation: Nice, France
• [Presentation] In vivo molecular imaging using humanized anti-beta2-glycoprotein I autoantibodies to detect arterial lesions in the atherosclerosis prone apoE-/- mice (2014)
  • Author(s): Matsunami Y, Matsuura E, et al(他5名)
  • Organizer: 9th International Congress on Autoimmunity
  • Place of Presentation: Nice, France
• [Presentation] Detection of oxidized lipoproteins accumulated in atherosclerosis lesions by imaging mass microscopy (iMS) (2013)
  • Author(s): Shen L, Matsuura E, et al(他8名)
  • Organizer: 6th Asian Congress of Autoimmunity
  • Place of Presentation: Hong Kong, China
• [Presentation] Infection and immunity-mediated atherosclerosis (2013)
  • Author(s): Matsuura E, Shen L, Lopez L
  • Organizer: 6th Asian Congress of Autoimmunity
  • Place of Presentation: Hong Kong, China
• [Presentation] A non-invasive PET/CT imaging procedure for diagnosing atherosclerotic lesions : immunologic targeting to oxLDL/beta2-glycoprotein I in the WHHL rabbits (2013)
  • Author(s): Sasaki T, Matsunami Y, Matsuura E
  • Organizer: 6th Asian Congress of Autoimmunity
  • Place of Presentation: Hong Kong, China
• [Presentation] Areautoantibodies against β2-glycoprotein I really atherogenic? : Answers from in vivo PET imaging studies in apolipoprotein E-deficient mice (2013)
  • Author(s): Matsunami Y, Matsuura E, et al(他5名)
  • Organizer: 6th Asian Congress of Autoimmunity
  • Place of Presentation: Hong Kong, China
• [Presentation] Recombinant domain V of beta2-glycoprotein I inhibits the formation of atherogenic oxLDL/β2-glycoprotein I complexes (2013)
  • Author(s): Liu Q, Matsuura E, et al(他3名)
  • Organizer: 6th Asian Congress of Autoimmunity
  • Place of Presentation: Hong Kong, China
• [Presentation] Pathological implication of autoantibodies againstβ2-glycoprotein I and a novel immune-theranostics in atherosclerosis (2013)
  • Author(s): Matsuura E, Lopez L
  • Organizer: 6th Asian Congress of Autoimmunity
  • Place of Presentation: Hong Kong, China
• [Presentation] Autoimmunity, oxidative inflammation, and β2-glycoprotein I in early atherogenesis (2013)
  • Author(s): Matsuura E
  • Organizer: Controversies and Rheumatology & Autoimmunity 2013
  • Place of Presentation: Budapest, Hungary
• [Presentation] Autoimmunity, oxidative inflammation, and β2-glycoprotein I in early atherogenesis (2013)
  • Author(s): Matsuura E
  • Organizer: Changchun International Forum for Cardiovascular Diseases and Dietary Nutrition
  • Place of Presentation: Jilin University, Changchun, China
• [Presentation] β2-Glycoprptein I and oxidative inflammation in atherogenesis of autoimmune atherothrombotic diseases (2012)
  • Author(s): Matsuura E
  • Organizer: International Congress on Autoimmunity
  • Place of Presentation: Granada, Spain
• [Presentation] Novel metabolic profiles in atherosclerosis-prone mice (2012)
  • Author(s): Shen L, Matsuura E, et al(他4名)
  • Organizer: 7th International Conference "Bioactive Okayama 2012"
  • Place of Presentation: Okayama
• [Book] Anti-β2-glycoprotein I antibodies. Autoantibodies (3rd edition). (Shoenfled Y, Gershwin ME, eds) (2014)
  • Author(s): Matsuura E, Lopez LR.
  • Publisher: Elsevier, Amsterdam, The Netherland