Development of new method detecting redox regulation against oxidative stress

• Project/Area Number: 23390152
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: The Tazuke Kofukai
• Principal Investigator: NAKAMURA Hajime 公益財団法人田附興風会, 医学研究所 第8研究部, 部長 (70303914)
• Co-Investigator(Kenkyū-buntansha): KASUNO Kenji 福井大学, 医学部, 准教授 (60455243)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2011: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
• Keywords: チオレドキシン / 急性腎障害 / 慢性腎臓病 / 酸化ストレス / 血液透析 / 末梢血リンパ球 / 過酸化水素 / バイオマーカー / レドックス / ヒト末梢血リンパ球 / mRNA / RT-PCR

Research Abstract

Thioredoxin is a redox-regulating protein which is induced by oxidative stress. We previously reported that plasma/serum levels of thioredoxin are elevated in diseases associated with oxidative stress. In this study, we found that urinary levels of thioredoxin are correlated with urinary levels of N-acetyl-beta-D-glucosamidase which is a marker for damage of renal tubules in chronic renal diseases. We have developed a new rapid method detecting the elevation of thioredoxin mRNA. It is possible to diagnose rapidly the pathological condition suffering from oxidative stress.

Research Products

• [Journal Article] Protective effects of oral administration of yeast thioredoxin against gastric mucosal injury (2014)
  • Author(s): Ｔａｋｅｔａｎｉ Ｙ， ｅｔ ａｌ．
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Smoking counteracts the favorable effect of exercise training on endothelial function in patients with type 2 diabetes (2013)
  • Author(s): Sato S, Nakayama N, Otsuki S, Tanaka S, Nakamura H, Koshiyama H, Nohara R
  • Journal Title: Japanese Clinical Medicine Volume: 4 Pages: 15-19
• [Journal Article] Treatment of nonalcoholic steatohepatitis with vitamins E and C : a pilot study (2013)
  • Author(s): Kawanaka M, Nishino K, Nakamura J, Suehiro M, Goto D, Urata N, Kawamoto T, Nakamura H, Yodoi J, Hino K, Yamada G
  • Journal Title: Hepat Med Volume: 5 Pages: 11-16
• [Journal Article] The protective role of the transmembrane thioredoxin-related protein TMX in inflammatory liver injury (2013)
  • Author(s): Matsuo Y, Irie K, Kiyonari H, Okuyama H, Nakamura H, Son A, Lopez-Ramos DA, Tian H, Oka S, Okawa K, Kizaki-Kondoh S, Masutani H, Yodoi J
  • Journal Title: Antiox. Redox Signal Volume: 18 Pages: 1263-1272
• [Journal Article] Treatment of nonalcoholic steatohepatitis with vitamins Eand C : a pilot study (2013)
  • Author(s): Kawanaka M, Nishino K, Nakamura J, Suehiro M, Goto D, Urata N, Oka T, Kawamoto H, Nakamura H, Yodoi J, Yamada G
  • Journal Title: Hepat. Med Volume: 5 Pages: 11-16
• [Journal Article] The protective role of transmembrane thioredoxin-related protein TMX in inflammatory liver injury (2013)
  • Author(s): Matsuo Y, Irie K, Kiyonari H, Okuyama H, Nakamura H, Son A, Lopez-Ramos DA, Tian H, Oka S, Okawa K, Kizaki-Kondoh S, Masutani H, Yodoi J
  • Journal Title: Antiox. Redox Signal Volume: 18 Pages: 1263-1272
• [Journal Article] Treatment of nonalcoholic steatohepatitis with vitamins E and C: a pilot study (2013)
  • Author(s): Ｋａｗａｎａｋａ Ｍ， ｅｔ ａｌ．
  • Journal Title: Hepat Med Volume: 5 Pages: 11-16
  • Peer Reviewed
• [Journal Article] The expression of thioredoxin01 in preterm delivery placenta (2012)
  • Author(s): Song J, Dong X, Chen Y, Chen G, Liang H, Nakamura H, Yodoi J, Bai J
  • Journal Title: Redox Report Volume: 17 Pages: 187-193
• [Journal Article] Nuclear expression of thioredoxin-1 in the invasion front is associated with outcome in patients with gallbladder carcinoma (2012)
  • Author(s): Nagano M, Hatakeyama K, Kai M, Nakamura H, Yodoi J, Asada Y, Chijiwa K
  • Journal Title: HPB Volume: 14 Pages: 573-582
• [Journal Article] Induction of endoplasmic reticulum stress and the modulation of thioredoxin-1 in formaldehyde-induced neurotoxicity (2012)
  • Author(s): Luo FC, Zhou J, Lv T, Qi L, Wang SD, Nakamura H, Yodoi J, Bai J
  • Journal Title: Neurotoxicology Volume: 33 Pages: 290-298
• [Journal Article] Geranylgeranylacetone protects mice against morphine-induced hyperlocomotion, rewarding effect, and withdrawal syndrome (2012)
  • Author(s): Luo FC, Qi L, Lv T, Wang SD, Liu H, Nakamura H, Yodoi J, Bai J
  • Journal Title: Free Radic. Biol. Med Volume: 52 Pages: 1218-1227
• [Journal Article] The expression of thioredoxin-1 in preterm delivery placenta (2012)
  • Author(s): Song J
  • Journal Title: Redox Report Volume: 17 Pages: 187-193
  • Peer Reviewed
• [Journal Article] Geranylgeranylacetone protectsmice against morphine-induced hyperlocomotion, rewarding effect, and withdrawal syndrome (2012)
  • Author(s): Luo FC, et al
  • Journal Title: Free Radic.Biol.Med. Volume: 52 Issue: 7 Pages: 1218-1227
  • DOI: 10.1016/j.freeradbiomed.2012.01.004
  • Peer Reviewed
• [Journal Article] Yeast thioredoxin-enriched extracts for mitigating the allergenicity of foods (2011)
  • Author(s): Taketani Y, Kinugasa K, Furukawa S, Nakamura H, Otsuki R, Yasuda H, Fujita T, Kanzaki K, Masutani H, Yodoi J
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 75 Pages: 1872-1879
  • NAID: 10029872460
• [Journal Article] Thioredoxin in allergic inflammation Int (2011)
  • Author(s): Ito W, Kobayashi N, Takeda M, Ueki S, Kayaba H, Nakamura H, Yodoi J, Chihara J
  • Journal Title: Arch. Allergy Immunol Volume: 155 Suppl 1 Pages: 142-146
• [Journal Article] Yeast Thioredoxin-Enriched Extracts for Mitigating the Allergenicity of Foods (2011)
  • Author(s): Taketani Y, et al
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 10 Pages: 1872-1879
  • DOI: 10.1271/bbb.100734
  • NAID: 10029872460
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] レドックス制御因子チオレドキシン研究の臨床展開 (2011)
  • Author(s): 糟野健司, ら
  • Journal Title: 臨床病理 Volume: 59 Pages: 189-195
  • NAID: 10029427045
• [Journal Article] Protective effects of oral administration of yeast thioredoxin against gastric mucosal injury Biosci
  • Author(s): Taketani Y, Kinugasa K, Kitajima R, Nishiumi S, Asada H, Nakamura H, Fujita T, Kanzaki K, Masutani H, Yodoi J
  • Journal Title: Biotech. Biochem Volume: (In press)
• [Presentation] 緩和ケア介入患者に対する食事サポート管理栄養士と看護しの協働による積極的な介入 (2014)
  • Author(s): 山田信子, 久保麻友子, 永原 守, 井上裕子, 金田恵美, 志水晃介, 長谷川美里, 鎗野 りか , 中村肇
  • Organizer: 第17回日本病態栄養学会年次学術集会
  • Place of Presentation: 大阪
• [Presentation] NST の栄養提案に対する実施率について~介入方式変更前後での比較~ (2014)
  • Author(s): 久保麻友子, 山田信子, 志水晃介, 山崎みどり, 阿賀千香子, 池上由利子, 井下春美, 藤本寛太, 内田洋一朗, 中村肇
  • Organizer: 第17回日本病態栄養学会年次学術集会
  • Place of Presentation: 大阪
• [Presentation] A comparative study on neoadjuvant chemotherapy for cervical cancer at high risk of relapse : A retrospective single-center pilot study (2013)
  • Author(s): Sakakibara A, Matsui K, Nagano T, Kono S, Nakamura H
  • Organizer: The 3rd Biennial Meeting of Asian Society of Gynecologic Oncology
  • Place of Presentation: Kyoto, Japan
• [Presentation] 入院患者における検査受付時及び実施時の患者確認方法~患者誤認防止対策としての無線バーコードリーダ導入の取り組み~ (2013)
  • Author(s): 山崎みどり, 加藤千春, 渡壁一雄, 辻野久美, 清水英樹, 西藤悠佳, 中村肇
  • Organizer: 第8回医療の質・安全学会学術集会
  • Place of Presentation: 東京
• [Presentation] 消化器癌との比較における肺癌化学療法の栄養評価 (2013)
  • Author(s): 長谷川美里, 山田信子, 鎗野 りか , 久保麻友子, 石床 学, 中村肇, 山内清明
  • Organizer: 第51回日本癌治療学会
  • Place of Presentation: 京都
• [Presentation] ヘリコバクター・ピロリ感染が健診結果に与える影響について (2013)
  • Author(s): 高森行宏, 米本智美, 近藤尚哉, 高橋綾子, 中村肇
  • Organizer: 第54回人間ドック学会学術大会
  • Place of Presentation: 浜松
• [Presentation] 胃癌術後の誤嚥性肺炎に対して口腔ケア介入が著効した一症例 (2013)
  • Author(s): 岩崎聡美, 内田洋一朗, 野田祥子, 井下春美, 山田信子, 久保麻友子, 阿賀千賀子, 池上由利子, 山崎みどり, 藤本寛太, 佐藤正人, 中村肇
  • Organizer: 第50回日本外科代謝栄養学会学術集会
  • Place of Presentation: 東京
• [Presentation] 当院の造影CT 検査における検査説明・チェック表・同意書の運用について (2013)
  • Author(s): 伊藤寿夫, 中村肇, 奥村亮介, 濱野孝志, 下村弘一, 今阪剛, 渡壁一雄, 奥井詩帆, 上藤裕里加
  • Organizer: 第63回日本病院学会
  • Place of Presentation: 新潟
• [Presentation] 心筋炎と抗酸化療法-レドックス制御蛋白チオレドキシンの有用性 (2012)
  • Author(s): 中村肇
  • Organizer: 第76回日本循環器病学会ランチョンセミナー
  • Year and Date: 2012-03-17
• [Presentation] 心筋炎と抗酸化療法-レドックス制御蛋白チオレドキシンの有用性- (2012)
  • Author(s): 中村肇
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡国際会議場(福岡県)(招待講演)
  • Year and Date: 2012-03-17
• [Presentation] 栄養サポートチーム加算算定後1 年間を振り返る=北野病院NST の栄養介入の傾向と今後の展望について= (2012)
  • Author(s): 久保麻友子, 山田信子, 山崎みどり, 阿賀千香子, 井下春美, 野村美香, 河崎祐貴子, 佐藤正人, 中村肇
  • Organizer: 第15回日本病態栄養学会年次学術集会
  • Place of Presentation: 京都
  • Year and Date: 2012-01-15
• [Presentation] 30 歳代男性における高感度CRP 検査の有用性と生活習慣との関連について (2012)
  • Author(s): 高森行宏, 米本智美, 松岡敦子, 近藤尚哉, 高橋綾子, 中村肇
  • Organizer: 第53回人間ドック学会学術大会
  • Place of Presentation: 東京
• [Presentation] 30歳代男性における高感度CRP検査の有用性と生活習慣との関連について (2012)
  • Author(s): 高森行宏
  • Organizer: 第５３回人間ドック学会学術大会
  • Place of Presentation: 東京都中央区 東京国際フォーラム
• [Presentation] 急性腎障害(AXI)の病因別診断に尿中チオレドキシンが有用である (2011)
  • Author(s): 糟野健司, ら
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山コンベンションセンター(岡山県)
  • Year and Date: 2011-11-18
• [Presentation] Monitoring of serum thioredoxin levels in NASH/NAFLD with repeated liver biopsies (2011)
  • Author(s): Kawanaka M, Oga C, Kawamoto H, Izumi A, Nakamura H, Yodoi J, Yamada G
  • Organizer: 2011 AASLD Annual Meeting, The Liver Meeting 2011
  • Place of Presentation: San Fransisco, CA, USA
• [Presentation] 当健康管理センターにおける高感度CRP 検査の有用性について (2011)
  • Author(s): 高森行宏, 米本智美, 松岡敦子, 近藤尚哉, 高橋綾子, 中村肇
  • Organizer: 第52回日本人間ドック学会学術大会
  • Place of Presentation: 大阪
• [Book] 血栓と循環19(1) (2011)
  • Author(s): 糟野健司, ら
  • Publisher: メディカルレビュー社
• [Book] 生体の抗酸化システム (3)チオレドキシン系 酸化ストレスの医学
  • Author(s): 中村肇
  • Publisher: (印刷中)