Elucidating the epigenetic effects of environmental chemicals and their impacts on next generation

• Project/Area Number: 23390157
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Hokkaido University
• Principal Investigator: KAORU Azumi 北海道大学, 環境健康科学研究教育センター, 客員研究員 (90221720)
• Co-Investigator(Kenkyū-buntansha): KISHI Reiko 北海道大学, 環境健康科学研究教育センター, 特任教授 (80112449)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Seiko 北海道大学, 大学院・医学研究, 助教 (30448831) ; IWANO Hidetomo 酪農学園大学, 獣医学部, 准教授 (60382488)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: 環境化学物質 / 環境疫学 / 前向きコホート / エピジェネティクス / DNAメチル化 / IGF2/H19 / LINE-1 / 低出生体重 / 臍帯血DNA

Research Abstract

We investigated the effects of prenatal exposure to environmental chemicals on IGF2/H19 and LINE1 methylation. Among 514 pregnant women recruited at Toho Hospital (Sapporo, Japan) during 2002-2005, 267 mother-infant pairs with cord blood samples were subjected to this study. DNA methylation was quantified by bisulfite pyrosequence. Concentrations of PFOS, PFOA, MEHP, Dioxins, PCBs in maternal blood and methylmercury in maternal hair were analyzed into their association with DNA methylation in multiple linear regression models. After adjustment for potential confounders, we observed hypomethylation at IGF2 by PFOA exposure (beta = -1.60, 95% CI: -2.98 to -0.22), hypomethylation at H19 by MEHP exposure (beta = -0.84, 95% CI: -1.68 to -0.00) and hypermethylation at LINE1 by methylmercury exposure (beta = 0.71, 95% CI: 0.07 to 1.36).

Research Products

• [Journal Article] Ten years of progress in the Hokkaido birth cohort study on environment and children's health : cohort profile-updated 2013 (2013)
  • Author(s): Kishi R, Kobayashi S, Ikeno T, Araki A, Miyashita C, Itoh S, Sasaki S, Okada E, Kobayashi S, Kashino I, et al
  • Journal Title: Environmental health and preventive medicine Volume: 18(6) Pages: 429-450
  • NAID: 50009002848
  • Peer Reviewed
• [Journal Article] Ten years of progress in the Hokkaido birth cohort study on environment and children's health : cohort profile--updated 2013. (2013)
  • Author(s): Kishi R, Kobayashi S, Ikeno T, Araki A, Miyashita C, Itoh S, Sasaki S, Okada E, Kobayashi S, Kashino I, Itoh K, Nakajima S ; Members of the Hokkaido Study on Environment and Children's Health.
  • Journal Title: Environmental Health and Preventive Medicine Volume: 18 Issue: 6 Pages: 429-450
  • DOI: 10.1007/s12199-013-0357-3
  • Peer Reviewed
• [Journal Article] 環境化学物質曝露の次世代影響の解明におけるエピジェネティクス研究 (2012)
  • Author(s): 安住薫, 小林祥子, 岸玲子
  • Journal Title: 北海道公衆衛生学雑誌 Volume: 26(2) Pages: 29-38
  • NAID: 120005297919
  • Peer Reviewed
• [Journal Article] 環境化学物質曝露の次世代影響の解明におけるエピジェネティクス研究 (2012)
  • Author(s): 安住薫
  • Journal Title: 北海道公衆衛生学雑誌 Volume: 26 Pages: 29-38
  • NAID: 120005297919
  • Peer Reviewed
• [Presentation] 環境化学物資の胎児期曝露が臍帯血IGF2DNA メチル化に及ぼす影響 (2013)
  • Author(s): 小林祥子, 安住薫, 宮下ちひろ, 小林澄貴, 佐々木成子, 石塚真由美, 岸玲子
  • Organizer: 第83回日本衛生学会学術総会
  • Place of Presentation: 金沢
• [Presentation] 環境化学物質の胎児期曝露が臍帯血IGF2 DNAメチル化に及ぼす影響 (2013)
  • Author(s): 小林祥子
  • Organizer: 第83回 日本衛生学会学術総会
  • Place of Presentation: 金沢大学鶴間キャンパス（金沢市）
• [Remarks]
  • URL: http://www.cehs.hokudai.ac.jp