Development of risk assessment methods for parkinson's disease using carbonylated protein, a protein oxidation product, as a predictive indicator
| Project/Area Number |
23390162
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
OIKAWA Shinji 三重大学, 医学(系)研究科(研究院), 准教授 (10277006)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TOMIMOTO Hidekazu 三重大学, 大学院・医学系研究科, 教授 (80324648)
MURATA Mariko 三重大学, 大学院・医学系研究科, 教授 (10171141)
YAMASHIMA Tetsumori 金沢大学, 医学系, 准教授 (60135077)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
|---|
| Keywords | パーキンソン病 / 酸化ストレス / プロテオミクス解析 / 酸化損傷タンパク質 / バイオマーカー / 血漿 / 黒質 / 神経細胞死 / 活性酸素種 |
|---|
| Research Abstract |
Oxidative stresses cause cell death of dopaminergic neurons of the substantia nigra (SN) in the brain of Parkinson's disease (PD). Therefore, we performed the identification and characterization of carbonylated protein, a protein oxidation product, in plasma of PD patients by using two-dimensional gel electrophoresis with immunochemical detection of protein carbonyls (2D Oxyblot). The specific oxidation level of complement component C9, vitamin D-binding protein, serum amyloid P-component, alpha-1-antichymotrypsin and fibrinogen beta chain were increased in plasma of PD patients, compared to the control. Therefore, some of these carbonylated proteins may serve as potential biomarkers helpful in the diagnosis of PD.
|
Report
(4 results)
Research Products
(80 results)
-
[Journal Article] Proteomic analysis of carbonylated proteins in the monkey substantia nigra after ischemia-reperfusion2014
Author(s)
Oikawa S, Kobayashi H, Kitamura Y, Zhu H, Obata K, Minabe Y, Dazortsava M, Ohashi K, Tada-Oikawa S, Takahashi H, Yata K, Murata M, Yamashima T
-
Journal Title
Related Report
Peer Reviewed
-
-
-
[Journal Article] Expression of proteins associated with adipocyte lipolysis was significantly changed in the adipose tissues of the obese spontaneously hypertensive/NDmcr-cp rat2014
Author(s)
Chang J, Oikawa S, Iwahashi H, Kitagawa E, Takeuchi I, Yuda M, Aoki C, Yamada Y, Ichihara G, Kato M, Ichihara S
-
Journal Title
Diabetol Metab Syndr
Volume: 6(1)
Issue: 1
Pages: 8-8
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Proteomic analysis of carbonylated proteins in the monkey substantia nigra after ischemia-reperfusion.2014
Author(s)
Oikawa S, Kobayashi H, Kitamura Y, Zhu H, Obata K, Minabe Y, Dazortsava M, Ohashi K, Tada-Oikawa S, Takahashi H, Yata K, Murata M, Yamashima T.
-
Journal Title
Free Radic Res.
Volume: 印刷中
Related Report
Peer Reviewed
-
-
-
[Journal Article] Inflammation-related DNA damage and expression of CD133 and Oct3/4 in cholangiocarcinoma patients with poor prognosis2013
Author(s)
Thanan R, Pairojkul C, Pinlaor S, Khuntikeo N, Wongkham C, Sripa B, Ma N, Vaeteewoottacharn K, Furukawa A, Kobayashi H, Hiraku Y, Oikawa S, Kawanishi S, Yongvanit P, Murata M
-
Journal Title
Free Radic Biol Med
Volume: 65
Pages: 1464-1472
DOI
Related Report
Peer Reviewed
-
[Journal Article] Altered gene and protein expression in liver of the obese spontaneously hypertensive/NDmcr-cp rat.2012
Author(s)
Chang J, Oikawa S, Ichihara G, Nanpei Y, Hotta Y, Yamada Y, Tada-Oikawa S, Iwahashi H, Kitagawa E, Takeuchi I, Yuda M, Ichihara S.
-
Journal Title
Nutr Metab (Lond).
Volume: 9（1）
Issue: 1
Pages: 87-87
DOI
NAID
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Proton pump inhibitors suppress iNOS-dependent DNA damage in Barrett's esophagus by increasing Mn-SOD expression2012
Author(s)
Thanan R, Ma N, Iijima K, Abe Y, Koike T, Shimosegawa T, Pinlaor S, Hiraku Y, Oikawa S, Murata M, Kawanishi S
-
Journal Title
Biochem Biophys Res Commun
Volume: 421(2)
Pages: 280-285
Related Report
Peer Reviewed
-
[Journal Article] Inflammation-induced protein carbonylation contributes to poor prognosis for cholangiocarcinoma2012
Author(s)
Thanan R, Oikawa S, Yongvanit P, Hiraku Y, Ma N, Pinlaor S, Pairojkul C, Wongkham C, Sripa B, Khuntikeo N, Kawanishi S, Murata M
-
Journal Title
Free Radic Biol Med
Volume: 52(8)
Pages: 1465-1472
Related Report
Peer Reviewed
-
-
-
[Journal Article] Nitrative DNA damage and Oct3/4 expression in urinary bladder cancer with Schistosoma haematobium infection2011
Author(s)
Ma N, Thanan R, Kobayashi H, Hammam O, Wishahi M, El Leithy T, Hiraku Y, Amro el-K, Oikawa S, Ohnishi S, Murata M, Kawanishi S
-
Journal Title
Biochem Biophys Res Commun
Volume: 414(2)
Pages: 344-349
Related Report
Peer Reviewed
-
-
-
[Journal Article] Proteomic identification of hippocampal proteins vulnerable to oxidative stress in excitotoxin-induced acute neuronal injury2011
Author(s)
Furukawa A, Kawamoto Y, Chiba Y, Takei S, Hasegawa-Ishii S, Kawamura N, Yoshikawa K, Hosokawa M, Oikawa S, Kato M, Shimada A
-
Journal Title
Neurobiol Dis
Volume: 43(3)
Pages: 706-714
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Proteomic identification of hippocampal proteins vulnerable to oxidative stress in excitotoxin-induced acute neuronal injury2011
Author(s)
A. Furukawa, Y. Kawamoto, Y. Chiba, S.Takei, S. Hasegawa-Ishii, N. Kawamura, K. Yoshikawa, M. Hosokawa, S. Oikawa, M. Kato, A. Shimada
-
Journal Title
Neurobiology of disease
Volume: 43
Issue: 3
Pages: 706-714
DOI
Related Report
Peer Reviewed
-
-
-
-
-
[Presentation] 上咽頭癌におけるメチル化DNAのゲノムワイド解析2014
Author(s)
Weilin Zhao, Shuming Wang, Kaoru Midorikawa, Ning Ma, Yusuke Hiraku, Shinji Oikawa, Mariko Murata
Organizer
第84回日本衛生学会学術総会
Place of Presentation
岡山市、岡山コンベンションセンター
Related Report
-
-
-
-
-
-
-
[Presentation] 上咽頭癌におけるmicroRNA異常とターゲット遺伝子の検討2013
Author(s)
Shumin Wang, Yingxi Mo, Kaoru Midorikawa, Zhe Zhang ,Guangwu Huang, Ning Ma, Yusuke Hiraku, Shinji Oikawa, Mariko Murata
Organizer
第72回日本癌学会総会
Place of Presentation
横浜市、パシフィコ横浜
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
