Identification of contributors in the low-template and mixed samples by forensic mathematical estimation and experimental analysis
Project/Area Number |
23390184
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Legal medicine
|
Research Institution | Kyoto University |
Principal Investigator |
TAMAKI Keiji 京都大学, 医学(系)研究科(研究院), 教授 (90217175)
|
Co-Investigator(Kenkyū-buntansha) |
鶴山 竜昭 京都大学, 医学(系)研究科(研究院), 准教授 (00303842)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
|
Keywords | DNA多型医学 / 混合試料 / STR / 血縁者 / DNA多型 |
Research Abstract |
Analyzing mixed or/and low-template DNA samples is challenging but inevitable in current forensic DNA test. We reevaluated autosomal STR multiplex typing kit and found that allele peak imbalance of heterozygote occurred even in a single person sample. Therefore, the determination of the heterozygous combination is not always reliable and the binary method which does not use the allelic peak heights still showed very powerful in mixed samples from 2 or 3 individuals. While it is often said that there is a risk that non-contributors cannot be excluded in a mixed sample, the simulated analysis using a large number of the mixed samples revealed that the first grade kin of the contributor is erroneously included as a contributor although its proportion is small. Moreover, 37 loci of autosomal STR were genotyped and the allele frequencies and the discriminating power were estimated in the Japanese population. Finally, the analytical procedure from mixed samples has been established.
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Report
(4 results)
Research Products
(40 results)