| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Research Abstract |
To understand how hematopoietic stem cells (HSCs) interact with bone marrow niche, we investigated functions of chemokine CXCL14 and cilia protein CMG-1, and searched for downstream effector molecules of the HSC-acting transcription factor Lhx2. We discovered that CXCL14 binds to CXCR4 with high affinity and completely inhibits the CXCL12-mediated migration of hematopoietic progenitor cells. However, number and frequency of bone marrow HSCs in CXCL14 knockout mice were similar as those in wild type mice. Same thing was true in bone marrows of HSC-specific CMG-1 conditional knockout mice. Lastly, we searched for membrane proteins that are relevant to the undifferentiated status of HSCs by using an Lhx2-trigerred HSC expansion culture of ES cells. As a result, we identified several candidate genes such as Biglycan, Esam1, and Jam3.
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