Systematic analyses of pathogenesis and cancer stem cell of pediatric solid tumors and development of novel therapeutic strategies
Project/Area Number |
23390269
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Pediatrics
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Research Institution | The University of Tokyo |
Principal Investigator |
TAKITA Junko 東京大学, 医学部附属病院, 准教授 (00359621)
|
Co-Investigator(Kenkyū-buntansha) |
SANADA Masashi 京都大学, 医学(系)研究科(研究院) (20529044)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
|
Keywords | 小児固形腫瘍 / Pediatric solid tumors / 横紋筋肉腫 / 次世代シークエンサー / 神経芽腫 / 医療・福祉 / 遺伝子 / 癌 / ゲノム / マイクロアレイ / がん幹細胞 / ポリコム群蛋白 / エクソーム解析 / ALK |
Research Abstract |
Pediatric solid tumors are one of the significant causes of death among children, and thus, development for new therapeutic strategy for intractable pediatric solid tumors is a global problem. To identify molecular targets of pediatric solid tumors and regulators of cancer stem cells, we performed target sequencing and exome sequencing using next generation sequencing technology. Target sequencing of 80 polycomb protein genes in 96 of neuroblastomas revealed that ASH1L mutations occurred in about 10% of cases. Thus, this gene is one of the candidate target for cancer stem cells regulators of neuroblastoma. In exome analysis of 16 cases of rhabdomyosarcoma (RMS), we identified FGFR4 pathway mutations in about 30% of all cases, and this pathway mutation was more frequently detected in embryonal subtype. Therefore, the inhibitor of FGFR4 pathway could be one of the promising therapeutic molecule for embryonal RMS.
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Report
(4 results)
Research Products
(184 results)
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[Journal Article] Biallelic DICER1 mutations in sporadic pleuropulmonary blastoma2014
Author(s)
Seki M, Yoshida K, Shiraishi Y, Shimamura T, Sato Y, Nishimura R, Okuno Y, Chiba K, Tanaka H, Kato K, Kato M, Hanada R, Nomura Y, Park MJ, Ishida T, Oka A, Igarashi T, Miyano S, Hayashi Y, Ogawa S, Takita J
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Journal Title
Cancer Res
Volume: 74
Issue: 10
Pages: 2742-9
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genome-wide approach to identify second gene targets for malignant rhabdoid tumors using high-density oligonucleotide microarrays2014
Author(s)
Takita J, Chen Y, Kato M, Ohki K, Sato Y, Ohta S, Sugita K, Nishimura R, Hoshino N, Seki M, Sanada M, Oka A, Hayashi Y, Ogawa S
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Journal Title
Cancer Sci
Volume: 105
Issue: 3
Pages: 258-64
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comparison of intravenous with oral busulfan in allogeneic hematopoietic stem cell transplantation with myeloablative conditioning regimens for pediatric acute leukemia2013
Author(s)
Kato M, Takahashi Y, Tomizawa D, Okamoto Y, Inagaki J, Koh K, Ogawa A, Okada K, Cho Y, Takita J, Goto H, et al
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Journal Title
Biol Blood Marrow Transplant
Volume: 19
Issue: 12
Pages: 1690-1694
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of genetic lesions in rhabdomyosarcoma using a high-density single nucleotide polymorphism array2013
Author(s)
Nishimura R, Takita J, Sato-Otsubo A, Kato M, Koh K, Hanada R, Tanaka Y, Kato K, Maeda D, Fukuyama M, Sanada M, Hayashi Y, Ogawa S
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Journal Title
Cancer Sci
Volume: 104
Issue: 7
Pages: 856-864
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Aggressive transformation of juvenile myelomonocytic leukemia associated with duplication of oncogenic KRAS in consequence of acquired uniparental disomy.2013
Author(s)
Kato M, Yasui N, Seki M, Kishimoto H, Sato-Otsubo A, Hasegawa D, Kiyokawa N, Hanada R. Ogawa S, Manabe A, Takita J, Koh K.
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Journal Title
J Pediatr.
Volume: 162
Issue: 6
Pages: 1285-1288
DOI
Related Report
Peer Reviewed
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[Journal Article] Biallelic DICER1 mutations in sporadic pleuropulmonary blastoma.2013
Author(s)
Seki M, Yoshida K, Shiraishi Y, Shimamura T, Sato Y, Nishimura R, Okuno Y, Chiba K, Tanaka H, Kato K, Kato M, Hanada R, Nomura Y, Park MJ, Ishida T, Oka A, Igarashi T, Miyano S, Hayashi Y, Ogawa S, Takita J
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Journal Title
Cancer Res.
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Mutational analyses of the ATP6V1B1 and ATP6V0A4 genes in patients with primary distal renal tubular acidosis.2013
Author(s)
Miura K, Sekine T, Takahashi K , Takita J, Harita Y, Ohki K, Park M-J, Hayashi Y, Tajima A, Ishihara M, Hisano M, Murai M, Igarashi T.
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Journal Title
Nephrol Dial Transplant.
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] ADORA2A polymorphism predisposes children to encephalopathy with febrile status epilepticus.2013
Author(s)
Shinohara M, Saitoh M, Nishizawa D, Ikeda K, Hirose S, Takanashi J, Takita J, Kikuchi K, Kubota M, Yamanaka G, Shiihara T, Kumakura A, Kikuchi M, Toyoshima M, Goto T et al.
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Journal Title
Neurology
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Aggressive transformation of juvenile myelomonocytic leukemia associated with duplication of oncogenic KRAS in consequence of acquired uniparental disomy.2013
Author(s)
Kato M, Yasui N, Seki M, Kishimoto H, Sato-Otsubo A, Hasegawa D, Kiyokawa N, Hanada R, .Ogawa S, Manabe A, Takita J, Koh K.
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Journal Title
J Pediatr.
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Second allogeneic hematopoietic stem cell transplantation for relapsed acute lymphoblastic leukemia in children2012
Author(s)
3. Kato M, Horikoshi Y, Okamoto Y, YTakahashi Y, Hasegawa D, Koh K, Takita J, Inoue M, Kigasawa H, Ogawa A, Sasahara Y, Kawa K, Yabe H, Sakamaki H, Suzuki R, Kato K.
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Journal Title
Bone Marrow Transplantat
Volume: 47
Issue: 10
Pages: 1307-1311
DOI
Related Report
Peer Reviewed
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[Journal Article] Aberrant activation of ALK kinase by a novel truncated form ALK protein in neuroblastoma2012
Author(s)
Okubo J, Takita J, Chen Y, Oki K, Nishimura R, Kato M, Sanada M, Hiwatari M, Hayashi Y, Igarashi T, Ogawa S.
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Journal Title
Oncogene
Volume: (in press)
Issue: 44
Pages: 4667-4676
DOI
Related Report
Peer Reviewed
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[Journal Article] CBL mutations in infant acute lymphoblastic leukaemia2012
Author(s)
6. Shiba N, Park MJ, Taki T, Takita J, Hiwatari M, Kanazawa T, Sotomatsu M, Ishii E, Arakawa H, Ogawa S, Hayashi Y
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Journal Title
Br J Haematol
Volume: 156
Issue: 5
Pages: 672-674
DOI
Related Report
Peer Reviewed
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[Journal Article] 骨髄移植後血栓性微小血管症の1例2012
Author(s)
苗代 有鈴, 三浦 健一郎, 張田 豊, 本村 あい, 樋渡 光輝, 滝田 順子, 井田 孔明, 宇於崎 宏, 五十嵐 隆
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Journal Title
日本小児腎不全学会雑誌
Volume: 32
Pages: 195-197
Related Report
Peer Reviewed
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[Journal Article] IDH1 and IDH2 mutations are rare in pediatric myeloid malignancies2011
Author(s)
Oki K, Takita J, Hiwatari M, Nishimura R, Sanada M, Okubo J, Adachi M, Sotomatsu M, Kikuchi A, Igarashi T, Hayashi Y, Ogawa S.
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Journal Title
Leukemia
Volume: 25
Issue: 2
Pages: 382-384
DOI
Related Report
Peer Reviewed
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[Presentation] Genetic Landscapes Of Childhood T-Cell Acute Lymphoblastic Leukemia2013
Author(s)
Seki M, Nishimura R, Hoshino N, Okuno Y, Yoshida K, Shiraishi Y, Sanada M, Miyano S, Hayashi Y, Ogawa S, Takita J
Organizer
American Society of Hematology 2013
Place of Presentation
New Orleans, U.S.A
Related Report
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[Presentation] The Genetic landscapes of rhabdomyosarcoma2013
Author(s)
Nishimura R, Yoshida K, Shiraishi Y, Okuno Y, Seki M, Hoshimo N, Sanada M, Hayashi Y, Miyano S, Ogawa S, Takita J
Organizer
The 45th Congress of the International Society of Paediatric Oncology
Place of Presentation
Hong Kong
Related Report
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[Presentation] Exome sequencing analysis in rhabdomyosarcoma including metastatic or relapsed tumors2013
Author(s)
Nishimura R, Yoshida K, Shiraishi Y, Okuno Y, Seki M, Hoshino N, Sanada M, Hayashi Y, Miyano S, Ogawa S, Takita J
Organizer
9th ASPR Congress
Place of Presentation
Malaysia
Related Report
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[Presentation] Genome-wide analyses of pleuropulmonary blastoma2013
Author(s)
Seki M, Nishimiura R, Hoshino N, Okuno Y, Yoshida K, Shiraishi Y, Sanada M, Kato K, Miyano S, Hayashi Y, Ogawa S, Takita J
Organizer
9th ASPR Congress
Place of Presentation
Malaysia
Related Report
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[Presentation] Genome-wide approach to identify gene targets of pleuropulmonary blastoma2013
Author(s)
Seki M, Nishimura R, Okuno Y, Yoshida K, Shiraishi Y, Sanada M, Kato K, Hayashi Y, Miyano S, Ogawa S, Takita J
Organizer
American Association for Cancer Research 2013
Place of Presentation
Washington DC, U.S.A.
Related Report
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[Presentation] Exome sequencing analysis in rhabdomyosarcoma including metastatic or relapsed tumors.2013
Author(s)
Nishimura R, Yoshida K, Shiraishi Y, Okuno Y, Seki M, Hoshino N, Sanada M, Hayashi Y, Miyano S, Ogawa S, Takita J
Organizer
9th ASPR Congress
Place of Presentation
Malaysia
Related Report
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[Presentation] Genome-wide analyses of pleuropulmonary blastoma.2013
Author(s)
Seki M, Nishimiura R, Hoshino N, Okuno Y, Yoshida K, Shiraishi Y, Sanada M, Kato K, Miyano S, Hayashi Y, Ogawa S, Takita J
Organizer
9th ASPR Congress
Place of Presentation
Malaysia
Related Report
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[Presentation] Identification of Somatic and Germline Mutations using Whole exome sequencing of hepatoblastoma.2013
Author(s)
Hoshino N, Nishimura R, Seki M, Okuno Y, Shiraishi Y, Sato Y, Yoshida K, Miyano S, Hahashi Y, Iwanaka T, Ogawa S, Takita J
Organizer
The 45th Congress of the International Society of Paediatric Oncology
Place of Presentation
Hong Kong
Related Report
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[Presentation] The Genetic landscapes of rhabdomyosarcoma.2013
Author(s)
Nishimura R, Yoshida K, Shiraishi Y, Okuno Y, Seki M, Hoshimo N, Sanada M, Hayashi Y, Miyano S, Ogawa S, Takita J
Organizer
The 45th Congress of the International Society of Paediatric Oncology
Place of Presentation
Hong Kong
Related Report
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[Presentation] Genome-wide approach to identify gene targets of pleuropulmonary blastoma.2013
Author(s)
Seki M, Nishimura R, Okuno Y, Yoshida K, Shiraishi Y, Sanada M, Kato K, Hayashi Y, Miyano S, Ogawa S, Takita J
Organizer
American Association for Cancer Research 2013
Place of Presentation
Washington DC, U.S.A
Related Report
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[Presentation] Genetic Landscapes Of Childhood T-Cell Acute Lymphoblastic Leukemia.2013
Author(s)
Seki M, Nishimura R, Hoshino N, Okuno Y, Yoshida K, Shiraishi Y, Sanada M, Miyano S, Hayashi Y, Ogawa S, Takita J
Organizer
American Society of Hematology 2013
Place of Presentation
New Orleans, U.S.A.
Related Report
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[Presentation] ターゲットキャプチャーを用いた神経芽腫におけるエピジェネティック関連遺伝子の網羅的ゲノム解析.2013
Author(s)
星野論子, 西村力, 関正史, 奥野友介, 吉田健一, 白石友一, 宮野悟, 林泰秀, 小川誠司, 岩中督, 滝田順子
Organizer
第50回 日本小児外科学会学術集会
Place of Presentation
東京
Related Report
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[Presentation] Genome-wide analysis of relapsed T cell acute lymphoblastic leukemia.2013
Author(s)
Seki M, Hoshino N, Nishimura R, Okuno Y, Shiraishi Y, Yoshida K, Kato M, Koh K, Hanada R, Miyano S, Hayashi Y, Ogawa S, Takita J
Organizer
The 75th Annual Meeting of the Japanese Society of Hematology
Place of Presentation
Sapporo
Related Report
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[Presentation] Whole-exome resequencing reveals novel pathogenetic gene mutations in pediatric AML.2013
Author(s)
Shiba N, Yoshida K, Okuno Y, Shiraishi Y, Nagata Y, Kon A, Chiba K, Tanaka H, Ohki K, Kato M, Terui K, Park MJ, Kanazawa T, Takita J, Kudo K, Arakawa H, Ito E, Sanada M, Miyano S, Ogawa S, Hayashi Y:
Organizer
The 75th Annual Meeting of the Japanese Society of Hematology
Place of Presentation
Sapporo
Related Report
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[Presentation] Whole-exome sequencing of advanced neuroblastomas using nextgeneration sequencer2012
Author(s)
Nishimura R, Takita J, Yoshida K, Shiraishi Y, Okubo J, Hoshino N, Sanada M, Hayashi Y, Miyano S, Igarashi T, Ogawa S
Organizer
The 8 th Congress of Asian Society for Pediatric Research
Place of Presentation
Seoul
Related Report
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[Presentation] Whole-exome sequencing of advanced neuroblastomas.2012
Author(s)
Nishimura R, Takita J, Yoshida K, Shiraishi Y, Hoshino N, Sanada M, Hayashi Y, Miyano S, Igarashi T, Ogawa S
Organizer
Advances Neuroblastoma Research Conference
Place of Presentation
Toronto, Canada
Related Report
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[Presentation] Whole-exome sequencing of advanced neuroblastomas using next-generation sequencer.2012
Author(s)
Nishimura R, Takita J, Yoshida K, Shiraishi Y, Okubo J, Hoshino N, Sanada M, Hayashi Y, Miyano S, Igarashi T, Ogawa S
Organizer
The 8th Congress of Asian Society for Pediatric Research
Place of Presentation
Seoul, Korea
Related Report
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[Presentation] Kasabach-Merritt syndromeを呈したKaposiform hemangioendotheliomaの1例2012
Author(s)
苗代 有鈴, 樋渡 光輝, 塩澤 亮輔, 本村 あい, 滝田 順子, 井田 孔明, 川嶋 寛, 赤羽 正章, 河野 博隆, 高澤 豊, 五十嵐 隆
Organizer
第115回日本小児科学会学術集会
Place of Presentation
福岡
Related Report
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[Presentation] T細胞性ALLにおける網羅的ゲノム解析
Author(s)
樋渡光輝, 関正史, 西村力, 星野論子, 奥野雄介, 白石悠一, 吉田健一, 林泰秀, 加藤元博, 康勝好, 花田良二, 宮野悟, 小川誠司, 滝田順子
Organizer
北関東小児がん学会
Place of Presentation
群馬
Related Report
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