Elucidation of the suppression mechanism to activation of cancer stem cells by hyperthermia
| Project/Area Number |
23390303
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Gunma University |
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Principal Investigator |
TAKAHASHI AKIHISA 群馬大学, 先端科学研究指導者育成ユニット, 准教授 (60275336)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | 放射線治療 / 温熱治療 / 感受性 / がん幹細胞 / DNA二本鎖切断 / 放射線治療学 |
|---|
| Research Abstract |
This study examined to clarify the heat sensitivity in X-ray resistant cells from human tongue cancer cells. Our results show that heat sensitivity was not significant difference between X-ray resistant cells and parental cells. The data of flow cytometory showed that cancer stem-like cells were significantly decreased after heating as compared with exposure to X-rays at iso-survival doses in both cells.
In addition, we examined the effect of the inhibitor of HR repair or NHEJ repair for DSBs on heat sensitivity. Heat sensitivity was not affected by NHEJ inhibitor. On the other hand, HR inhibitor was able to enhance heat sensitivity. These results suggest that the heat sensitivity may be enhanced by the suppression of HR repair. In addition, these findings provide support for the concept that heat may lead to the induction of DSBs.
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Report
(4 results)
Research Products
(86 results)
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[Journal Article] Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632 in lung adenocarcinoma A549 cells2014
Author(s)
Kazutoshi Murata, Shin-ei Noda, Takahiro Oike, Akihisa Takahashi, Yukari Yoshida, Yoshiyuki Suzuki, Tatsuya Ohno, Tomoo Funayama, Yasuhiko Kobayashi, Takeo Takahashi, Takashi Nakano
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Journal Title
Journal of Radiation Research
Volume: 55
Pages: 658-64
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Depression of p53-independent Akt survival signals in human oral cancer cells bearing mutated p53 gene after exposure to high-LET radiation2012
Author(s)
Yosuke Nakagawa,Akihisa Takahashi,Atsuhisa Kajihara,Nobuhiro Yamakawa,Yuichiro Imai,Ichiro Ota,Noritomo Okamoto,Eiichiro Mori,Taichi Noda,Yoshiya Furusawa,Tadaaki Kirita,Takeo Ohnishi
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Journal Title
Biochem Biophys Res Commun
Volume: 423 (4)
Pages: 654-60
DOI
Related Report
Peer Reviewed
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[Journal Article] siRNA targeted for nbs1 enhances heat sensitivity in human anaplastic thyroid carcinoma cells2011
Author(s)
Okamoto N, Takahashi A, Ota I, Ohnishi K, Mori E, Kondo N, Noda T, Nakagawa Y, Uemura H, Yane K, Hosoi H, Ohnishi T.
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Journal Title
Int J Hyperthermia
Volume: 27
Pages: 297-304
DOI
Related Report
Peer Reviewed
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[Presentation] 放射線抵抗性がん幹細胞様細胞に対する温熱感受性2013
Author(s)
高橋昭久, 馬洪玉, 久保誠, 中川彰子, 吉田由香里, 桑原義和, 福本学
Organizer
第17回関東ハイパーサーミア研究会/全身ハイパーサーミア研究会合同学術研究会
Place of Presentation
東京厚生年金病院 (東京都)
Year and Date
2013-03-02
Related Report
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[Presentation] DNA二本鎖切断修復を分子標的としたLET依存的な殺細胞効果.2013
Author(s)
久保誠, 高橋昭久, 馬洪玉, 中川彰子, 吉田由香里, 古澤佳也, 小林泰彦, 舟山知夫, 金井達明, 大野達也,中野隆史.
Organizer
第3回国際放射線神経生物学会大会
Place of Presentation
沖縄 万国津梁館
Related Report
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[Presentation] ヒト肺がん細胞の炭素線治療における標的としてのNHEJ修復.2013
Author(s)
馬洪玉, 高橋昭久, 久保誠, 中川彰子, 吉田由香里, 古澤佳也, 小林泰彦, 舟山知夫, 金井達明, 大野達也, 中野隆史.
Organizer
第15回癌治療増感研究シンポジウム
Place of Presentation
奈良 猿沢荘
Related Report
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[Presentation] LET依存的な殺細胞効果におよぼすDNA二本鎖切断修復の役割.2013
Author(s)
高橋昭久, 久保誠, 馬洪玉, 中川彰子, 吉田由香里, 古澤佳也, 小林泰彦, 舟山知夫, 金井達明, 大野達也,中野隆史.
Organizer
第6回 Quantum Medicine 研究会
Place of Presentation
茨城 茨城大学
Related Report
Invited
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[Presentation] DNA 修復を標的とした温熱増感研究2011
Author(s)
高橋昭久, 森英一朗, 近藤夏子, 野田太一, 梶原淳久, 長谷川正俊, 大西武雄
Organizer
第17回癌治療増感研究シンポジウム
Place of Presentation
赤門鍼灸柔整専門学校(宮城県)
Year and Date
2011-06-08
Related Report
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