• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effect of heavy ion beam irradiation on radioresistance and differentiation of cancer stem cells in neuronal tumors

Research Project

Project/Area Number 23390304
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionNara Medical University

Principal Investigator

HASEGAWA Masatoshi  奈良県立医科大学, 医学部, 教授 (50251111)

Co-Investigator(Kenkyū-buntansha) OHNO Tatsuya  群馬大学, 重粒子線医学研究センター, 教授 (10344061)
KATO Shingo  埼玉医科大学, 国際医療センター, 教授 (00370875)
INOUE Kazuya  奈良県立医科大学, 医学部, 研究員 (20596952)
KATAYAMA Emiko  奈良県立医科大学, 医学部, 助教 (00596950)
Research Collaborator 若月 優  放射線医学総合研究所
村上 健  放射線医学総合研究所
吉田 由香里  群馬大学, 大学院・医学系研究科
中野 隆史  群馬大学, 大学院・医学系研究科
石内 勝吾  琉球大学, 医学部
武田 麻衣子  奈良県立医科大学, 医学部
藤谷 信将  奈良県立医科大学, 医学部
藤村 浩子  奈良県立医科大学, 医学部
川島 真紀  奈良県立医科大学, 医学部
榎本 喜久子  奈良県立医科大学, 医学部
内田 雅代  奈良県立医科大学, 医学部
浅川 勇雄  奈良県立医科大学, 医学部
玉本 哲郎  奈良県立医科大学, 医学部
大林 千穂  奈良県立医科大学, 医学部
大西 武雄  奈良県立医科大学, 医学部
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Keywords癌幹細胞 / 神経幹細胞 / 放射線抵抗性 / アポトーシス / p53 / 炭素イオン線 / Ki-67 / CD133 / cDNAアレイ解析 / 膠芽腫
Research Abstract

The purpose is to investigate the radiobiological effect of heavy ion (CI) beams on radioresistance and differentiation of human tumor cells, especially cancer stem cells. Human ependymoblastoma with wild-type p53 and glioblastoma with mutant-type p53 were transplanted into nude mice and irradiated with CI beams or 150-kV X-rays. Tumors were excised for cDNA microarray analysis 6- 48 hours or 6 weeks after 2-24 Gy irradiation. CD133, CD44, Nestin, p53, PTEN, Ki67, and GFAP expressions were evaluated immunohistochemically. In ependymoblastoma, p53 signaling and apoptosis-related pathways were suggested to be involved following irradiation. The tumors showed a significant decrease in growth fraction and a slight increase of the CD133-positive rate after high-dose irradiation. These results suggest that CD133-positive and Ki-67-negative cells may be resistant to both X-rays and CI beams.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (26 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results) Presentation (16 results)

  • [Journal Article] The biochemical recurrence-free rate in patients who underwent prostate low-dose-rate brachytherapy, using two different definitions2014

    • Author(s)
      Tanaka N, Asakawa I, Katayama E, Hirayama A, Hasegawa M, Konishi N, Fujimoto K
    • Journal Title

      Radiat Oncol

      Volume: 9(1) Issue: 1 Pages: 107-107

    • DOI

      10.1186/1748-717x-9-107

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Periodical assessment of genitourinary and gastrointestinal toxicity in patients who underwent prostate low-dose-rate brachytherapy2013

    • Author(s)
      Tanaka N, Asakawa I, Anai S, Hirayama A, Hasegawa M, Konishi N, Fujimoto K
    • Journal Title

      Radiat Oncol

      Volume: 8 Issue: 1 Pages: 25-25

    • DOI

      10.1186/1748-717x-8-25

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of FDG-PET for Defining Gross Tumor Volume of Head and Neck Cancer2013

    • Author(s)
      梶谷千香枝
    • Journal Title

      Journal of Radiation Research

      Volume: Epub ahead of print Issue: 4 Pages: 1-8

    • DOI

      10.1093/jrr/rrs131

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pathological and clinical impact of neoadjuvant chemoradiotherapy using full-dose gemcitabine and concurrent radiation for resectable pancreatic cancer2013

    • Author(s)
      Sho M, Akahori T, Tanaka T, Kinoshita S, Tamamoto T, Nomi T, Yamato I, Hokuto D, Yasuda S, Kawaguchi C, Nishiofuku H, Marugami N, Enomonoto Y, Kasai T, Hasegawa M, Kichikawa K, Nakajima Y
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 20(2) Issue: 2 Pages: 197-205

    • DOI

      10.1007/s00534-012-0532-8

    • NAID

      10031168210

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Minimal percentage of dose received by 90% of the urethra (%UD90) is the most significant predictor of PSA bounce in patients who underwent low-dose-rate brachytherapy (LDR-brachytherapy) for prostate cancer2012

    • Author(s)
      Tanaka N, Asakawa I, Fujimoto K, Anai S, Hirayama A, Hasegawa M, Konishi N, Hirao Y
    • Journal Title

      BMC Urol

      Volume: 12 Issue: 1 Pages: 28-28

    • DOI

      10.1186/1471-2490-12-28

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preoperative concurrent chemoradiotherapy for stages II-IV oral squamous cell carcinoma : a retrospective analysis and the future possibility of this treatment strategy2012

    • Author(s)
      Kirita T, Yamanaka Y, Imai Y, Yamakawa N, Aoki K, Nakagawa Y, Yagyuu T, Hasegawa M
    • Journal Title

      Int J Oral Maxillofac Surg

      Volume: 41(4) Issue: 4 Pages: 421-8

    • DOI

      10.1016/j.ijom.2011.12.003

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Axillary lymph node accumulation on FDG-PET/CT after influenza vaccination2012

    • Author(s)
      Shirone N, Shinkai T, Yamane T, Uto F, Yoshimura H, Tamai H, Imai T, Inoue M, Kitano S, Kichikawa K, Hasegawa M
    • Journal Title

      Ann Nucl Med

      Volume: 26(3) Issue: 3 Pages: 248-52

    • DOI

      10.1007/s12149-011-0568-x

    • NAID

      10030623867

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intra-arterial chemoinfusion prior to chemoradiotherapy with full-dose systemic gemcitabine for management of locally advanced pancreatic cancer2011

    • Author(s)
      Tanaka T, Nishiofuku H, Tamamoto T, Sho M, Anai H, Sueyoshi S, Sakaguchi H, Hasegawa M, Nakajima Y, Kichikawa K
    • Journal Title

      Anticancer Res

      Volume: 31(11) Pages: 3909-12

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bone scan can be spared in asymptomatic prostate cancer patients with PSA of <=20 ng/ml and Gleason score of <=6 at the initial stage of diagnosis2011

    • Author(s)
      Tanaka N, Fujimoto K, Shinkai T, Nakai Y, Kuwada M, Anai S, Miyake M, Hirayama A, Hasegawa M, Hirao Y
    • Journal Title

      Jpn J Clin Oncol

      Volume: 41(10) Issue: 10 Pages: 1209-1213

    • DOI

      10.1093/jjco/hyr118

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A comparison of surgery and radiation therapy for cT1 esophageal squamous cell carcinoma2011

    • Author(s)
      Matsumoto S, Takayama T, Tamamoto T, Wakatsuki K, Enomoto K, Tanaka T, Migita K, Asakawa I, Hasegawa M, Nakajima Y
    • Journal Title

      Dis Esophagus

      Volume: 24(6) Issue: 6 Pages: 411-7

    • DOI

      10.1111/j.1442-2050.2010.01163.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 粒子線治療の放射線生物学2013

    • Author(s)
      長谷川正俊 , 他
    • Organizer
      日本放射線腫瘍学会第26回学術大会
    • Place of Presentation
      青森
    • Year and Date
      2013-10-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Changes of Gene Expression Profiles and Cancer Stem Cell Markers in Brain Tumors Following Carbon Ion Beam Irradiation2013

    • Author(s)
      Hasegawa M , et al
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2013-10-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Cancer Stem Cell Markers and Gene Expression Profiles in Human Brain Tumors Following Carbon Ion Beam Irradiation2013

    • Author(s)
      Hasegawa M, et al
    • Organizer
      第55回アメリカ放射線腫瘍学会(ASTRO)
    • Place of Presentation
      アメリカ合衆国アトランタ
    • Year and Date
      2013-09-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Gene Expression Profiles Induced by Carbon Ion Beams and X-rays in Human Brain Tumors2012

    • Author(s)
      Hasegawa M, et al
    • Organizer
      第54回アメリカ放射線腫瘍学会(ASTRO)
    • Place of Presentation
      アメリカ合衆国ボストン
    • Year and Date
      2012-10-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Gene expression profiles induced by carbon ion beams in glioblastoma : close relation to anti-apoptosis and survival2012

    • Author(s)
      長谷川正俊, 他
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      2012-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 放射線抵抗性の p53 変異型膠芽腫における炭素線照射による遺伝子発現の検討2012

    • Author(s)
      長谷川正俊, 他
    • Organizer
      第71回日本医学放射線学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2012-04-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 放射線治療とアポトーシス2011

    • Author(s)
      長谷川正俊, 他
    • Organizer
      日本放射線影響学会第54回大会
    • Place of Presentation
      神戸
    • Year and Date
      2011-11-17
    • Related Report
      2013 Final Research Report
  • [Presentation] IAP and NF-kappaB activation induced by carbon ion beams in human radioresistant glioblastoma cells with mutant-type p53 in vivo2011

    • Author(s)
      Hasegawa M, et al
    • Organizer
      第53回アメリカ放射線腫瘍学会(ASTRO)
    • Place of Presentation
      アメリカ合衆国マイアミ
    • Year and Date
      2011-10-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Cancer Stem Cell Markers and Gene Expression Profiles in Human Brain Tumors Following Carbon Ion Beam Irradiation

    • Author(s)
      長谷川正俊
    • Organizer
      第55回米国放射線腫瘍学会(ASTRO)
    • Place of Presentation
      ジョージアワールドコングレスセンター(米国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Changes of Gene Expression Profiles and Cancer Stem Cell Markers in Brain Tumors Following Carbon Ion Beam Irradiation

    • Author(s)
      長谷川正俊
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 粒子線治療の放射線生物学

    • Author(s)
      長谷川正俊
    • Organizer
      日本放射線腫瘍学会第26回学術大会
    • Place of Presentation
      ホテル青森(青森市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Gene Expression Profiles Induced by Carbon Ion Beams and X-rays in Human Brain Tumors

    • Author(s)
      長谷川正俊
    • Organizer
      第54回米国放射線腫瘍学会(ASTRO)
    • Place of Presentation
      ボストンコンベンションエキシビションセンター(米国)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 放射線抵抗性のp53変異型膠芽腫における炭素線照射による遺伝子発現の検討

    • Author(s)
      長谷川正俊
    • Organizer
      第71回日本医学放射線学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Gene expression profiles induced by carbon ion beams in glioblastoma: close relation to anti-apoptosis and survival

    • Author(s)
      長谷川正俊
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ホテルロイトン札幌(札幌市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] IAP and NF-kappaB activation induced by carbon ion beams in human

    • Author(s)
      長谷川正俊
    • Organizer
      第53回米国放射線腫瘍学会(ASTRO)
    • Place of Presentation
      マイアミビーチコンベンションセンター(米国)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 放射線治療とアポトーシス

    • Author(s)
      長谷川正俊
    • Organizer
      日本放射線影響学会 第54回大会
    • Place of Presentation
      神戸商工会議所(神戸市)
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi