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Development of a new rapid immunohistochemical staining method using AC electric field

Research Project

Project/Area Number 23390311
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionAkita University

Principal Investigator

MINAMIYA Yoshihiro  秋田大学, 医学(系)研究科(研究院), 教授 (30239321)

Co-Investigator(Kenkyū-buntansha) AKAGAMI Yoichi  , 秋田県産業技術センター, 副センター長 (00373217)
谷野 美智枝  北海道大学, 医学(系)研究科(研究院), 講師 (90360908)
小川 純一  秋田大学, 医学(系)研究科(研究院), 教授 (20112774)
Co-Investigator(Renkei-kenkyūsha) TANINO Mishie  北海道大学, 大学院医学系研究科, 講師 (90360908)
Research Collaborator NANJO Hiroshi  
NAKAMURA Ryuta  
TANAKA Shinya  
ITO Tomoo  
KAGAYA Masami  
KONNO Hayato  
SASAJIMA Toshio  
Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords免疫組織染色 / 癌 / 手術 / 術中迅速診断 / リンパ節微小転移 / 肺癌 / 脳腫瘍 / 病理 / 免疫祖織染色 / リンパ節転移 / 電界撹拌技術
Outline of Final Research Achievements

For the intraoperative diagnosis of lymph-node micro-metastases with immunohistochemical staining, the staining time should be shortened. We invented a new method that allows to reduce the immunohistochemical-staining time by applying an electric field to the tissue sections. We can complete immunohistochemical staining which usually requires 2 hours, within 12 minutes using this method. We demonstrated that this method was useful for the intraoperative detection of lymph node micrometastases of patients with lung cancer. We also demonstrated that this method is useful for the grading of the glioma during surgery. We tried this method to the decision making of the surgery for lung cancer and brain tumor. We also tried this method to the decision making of the surgery of gastroenterological, gynecologic, pediatric cancer and cancer of otorhinolaryngology. And then, we were able to demonstrate the usefulness of this method for the decision making of these surgeries

Report

(4 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (8 results)

All 2015 2014 2011

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (3 results) (of which Invited: 3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors.2015

    • Author(s)
      Tanino M, Sasajima T, Nanjo H, Akesaka S, Kagaya M, Kimura T, Ishida Y, Oda M, Takahashi M, Sugawara T, Yoshioka T, Nishihara H, Akagami Y, Goto A, Minamiya Y, Tanaka S; R-IHC Study Group.
    • Journal Title

      Brain Tumor Pathol.

      Volume: 32 Issue: 1 Pages: 12-19

    • DOI

      10.1007/s10014-014-0188-y

    • NAID

      120005682120

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 迅速免疫染色2014

    • Author(s)
      南條 博、吉岡年明、南谷佳弘、赤上陽一
    • Journal Title

      病理と臨床

      Volume: 32 Pages: 54-58

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel immunohistochemical staining method allows ultrarapid detection of lymph node micrometastases while conserving antibody2011

    • Author(s)
      Toda H, Minamiya Y, et.al.
    • Journal Title

      Acta Histochem Cytochem

      Volume: 44 Pages: 133-139

    • NAID

      130000722777

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 超高速免疫組織化学2015

    • Author(s)
      南谷佳弘
    • Organizer
      日本臨床検査自動化学会
    • Place of Presentation
      青森県弘前市
    • Year and Date
      2015-04-25
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 迅速免疫染色装置 (R-IHCラピート)の開発とリンパ節微小転移診断2014

    • Author(s)
      南谷佳弘、齋藤元、今井一博、佐藤雄亮、工藤智司、齋藤芳太郎、寺田かおり、中村竜太、赤上陽一
    • Organizer
      第16回SNNS研究会学術集会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2014-09-19 – 2014-09-20
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 呼吸器疾患のBio-Imaging ーPET/CTによる肺癌の診断と治療効果判定の進歩、肺癌のリンパ節転移診断N診断モダリティー オーバービュー2014

    • Author(s)
      今野隼人、井一博、齊藤元、工藤智司、齋藤芳太郎、南谷佳弘
    • Organizer
      第55回日本呼吸器学会学術講演会/日本核医学会分科会呼吸器核医学研究会
    • Place of Presentation
      東京
    • Year and Date
      2014-04-17 – 2014-04-19
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Patent(Industrial Property Rights)] 電界洗浄法2014

    • Inventor(s)
      赤上陽一、加賀谷昌美、中村竜太、池田洋、南谷佳弘、南條博
    • Industrial Property Rights Holder
      秋田県、国立大学法人秋田大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-009634
    • Filing Date
      2014-01-22
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 電界撹拌向けインジケーター付きはっ水リング2014

    • Inventor(s)
      赤上陽一、加賀谷昌美、中村竜太、池田洋、南谷佳弘、南條博
    • Industrial Property Rights Holder
      秋田県、国立大学法人秋田大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-009629
    • Filing Date
      2014-01-22
    • Related Report
      2013 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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