Translational research for lung regenerative treatment by cell transplantation

• Project/Area Number: 23390312
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Chiba University
• Principal Investigator: YOSHINO Ichiro 千葉大学, 医学(系)研究科(研究院), 教授 (40281547)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Shigetoshi 千葉大学, 大学院・医学研究院, 准教授 (90334200) ; MIZOBUCHI Teruaki 千葉大学, 医学部付属病院, 講師 (50569861) ; SAKAIRI Yuichi 千葉大学, 医学部付属病院, 医員 (30551949)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 肺再生 / 細胞移植 / 代償性肺成長 / iPS細胞 / II型肺胞上皮細胞 / 肺切除

Research Abstract

Type II pneumocytes prepared from male F344 rats were effectively integrated in the alveolar tissues of pneumonectomized rats (female), alveolar density of which were recognized to be enhanced (published).As like the above experiments using rats, compensatory lung growth is to be investigated in mice (B6). And also investigated is a relationship between histologic morphology and radiologic density obtained from computed tomography (on going).Human type II pneumocytes were prepared from clinically resected lung, and examined for capability to differentiate to type I pneumocytes and to be integrated in the lung tissue (submitted for publication).To prepare the future lung regeneration medicine, a novel method for estimated quantity of pulmonary tissue was developed (published).Human and murine iPS cells are to be subjected to culture for development of type II pneumocytes (on going).

Research Products

• [Journal Article] Spirometric and radiological evaluation of the remnant lung long after major pulmonary resection: can compensatory phenomena be recognized in clinical cases? (2014)
  • Author(s): Mizobuchi T, Wada H, Sakairi Y, Suzuki H, Nakajima T, Tagawa T, Iwata T, Motoori K, Yoshida S, Yoshino I
  • Journal Title: Surgery Today Volume: in press Issue: 9 Pages: 1735-1743
  • DOI: 10.1007/s00595-013-0702-6
  • Peer Reviewed
• [Journal Article] Increase of bone-morphogenetic protein-7 expressing pulmonary resident cells in pneumonectomized rats. (2014)
  • Author(s): Ohba T, Wada H, Yoshino I, Yoshida S, Tagawa T, Shoji F, Yamazaki K, Maehara Y
  • Journal Title: Surgery Today Volume: 44 Issue: 2 Pages: 324-331
  • DOI: 10.1007/s00595-013-0604-7
  • Peer Reviewed
• [Journal Article] Spirometric and radiological evaluation of the remnant lung long after major pulmonary resection : can compensatory phenomena be recognized in clinical cases? (2013)
  • Author(s): Mizobuchi T, Wada H, Sakairi Y, Suzuki H, Nakajima T, Tagawa T, Iwata T, Motoori K, Yoshida S, Yoshino I
  • Journal Title: Surgery Today Surg Today
  • Peer Reviewed
• [Journal Article] Increase of bone-morphogenetic protein-7 expressing pulmonary resident cells in pneumonectomized rats (2013)
  • Author(s): Ohba T, Wada H, Yoshino I, Yoshida S, Tagawa T, Shoji F, Yamazaki K, Maehara Y
  • Journal Title: Surgery Today Volume: 44 Pages: 324-331
  • Peer Reviewed
• [Journal Article] Radiologic evaluation for volume and weight of remnant lung in living lung donors (2013)
  • Author(s): Mizobuchi T, Chen F, Yoshino I, Iwata T, Yoshida S, Bando T, Date H
  • Journal Title: J Thorac Cardiovasc Surg Volume: 146 Pages: 1253-1258
  • Peer Reviewed
• [Journal Article] Radiologic evaluation for volume and weight of remnant lung in living lung donors. (2013)
  • Author(s): Mizobuchi T, Chen F, YoshinoI, Iwata T, Yoshida S, Bando T, Date H.
  • Journal Title: J Thorac Cardiovasc Surg Volume: 146 Pages: 1253-1258
  • Peer Reviewed
• [Journal Article] Transplantation of alveolar type II cells stimulates lung regeneration during compensatory lung growth in adult rats (2012)
  • Author(s): Wada H, Y oshida S, Suzuki H, Sakairi Y, Mizobuchi T, Komura D, Sato Y, Y okoi S, Y oshino I
  • Journal Title: J Thorac Cardiovasc Surg Volume: 143(3) Pages: 711-719
  • Peer Reviewed
• [Journal Article] Transplantation of alveolar type II cells stimulates lung regeneration during compensatory lung growth in adult rats (2012)
  • Author(s): Wada H, Yoshida S, Suzuki H, Sakairi Y, Mizobuchi T, Komura D, Sato Y, Yokoi S, Yoshino I
  • Journal Title: J Thorac Cardiovasc Surg Volume: 143 Pages: 711-719
  • Peer Reviewed
• [Presentation] 肺外科における新たなバイオロジーの探求：肺は肺切除後にどのように反応するのか？ (2013)
  • Author(s): 溝渕輝明,畑敦,豊田行英,田中教久,山本高義,尹貴正,鎌田稔子,森本淳一,坂入祐一,和田啓伸,鈴木秀海,山田義人,岩田剛和,田川哲三,千代雅子,吉田重利,吉野一郎
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] ヒトII型肺胞上皮細胞の異種経気道移植による肺再生モデルの作成 (2012)
  • Author(s): 坂入祐一、和田啓伸、吉田成利、稲毛輝長、森本淳一、石橋史博、岩田剛和、千代雅子、溝渕輝明、米谷卓郎、守屋康充、星野英久、本橋新一郎、吉野一郎
  • Organizer: 第112回日本外科学会定時学術集会
  • Place of Presentation: 千葉
• [Presentation] COPD症例は、肺切除後の"代償性肺成長"を示さない "仮想肺組織量"を指標とした検証 (2012)
  • Author(s): 溝渕 輝明, 吉田 成利, 稲毛 輝長, 森本 淳一, 坂入 祐一, 石橋 史博, 千代 雅子, 米谷 卓郎, 岩田 剛和, 守屋 康充, 星野 英久, 吉野 一郎
  • Organizer: 第112回日本外科学会定時学術集会
  • Place of Presentation: 千葉
• [Presentation] II型細胞上皮を用いた肺再生・保護療法を目指した基礎研究 (2012)
  • Author(s): 坂入祐一,和田啓伸、吉田成利、豊田行英、畑敦、山本高義、森本淳一、鈴木秀海、山田義人、岩田剛和、田川哲三、千代雅子、溝渕輝明、本橋新一郎、吉野一郎
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] 呼吸器外科手術における肺再生・ (2012)
  • Author(s): 坂入祐一,和田啓伸、吉田成利、豊田行英、畑敦、山本高義、田中教久、鎌田稔子、森本淳一、長門芳、鈴木秀海、山田義人、岩田剛和、田川哲三、千代雅子、溝渕輝明、本橋新一郎、吉野一郎
  • Organizer: 第29回日本呼吸器外科学会総会
  • Place of Presentation: 秋田
• [Presentation] 全摘術後残存肺における肺再生にはII型肺胞上皮細胞の補充が必要である (2011)
  • Author(s): 和田啓伸, 吉田成利, 鈴木秀海, 坂入祐一, 石橋史博, 田村創, 岩田剛和, 溝渕輝明, 守屋康充, 星野英久, 岡本龍郎, 本橋新一郎, 河村大輔, 横井左奈, 吉野一郎
  • Organizer: 第111回日本外科学会学術集会
  • Place of Presentation: (誌上開催)
  • Year and Date: 2011-05-25
• [Presentation] ヒト手術肺からの肺再生療法を目的としたcell sour ceの分離および解析 (2011)
  • Author(s): 坂入祐一, 和田啓伸, 鈴木秀海, 吉田成利, 石橋史博, 田村創, 岩田剛和, 溝渕輝明, 守屋康光, 星野英久, 岡本龍郎, 本橋新一郎, 吉野一郎
  • Organizer: 第64回日本胸部外科学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] II型肺胞上皮細胞を用いた肺再生療法の開発代償性肺成長モデルによる基礎実験 (2011)
  • Author(s): 和田啓伸, 吉田成利, 鈴木秀海, 坂入祐一, 石橋史博, 田村創, 岩田剛和, 溝渕輝明, 守屋康光, 星野英久, 岡本龍郎, 本橋新一郎, 河村大輔, 横井左奈, 吉野一郎
  • Organizer: 第47回日本移植学会
  • Place of Presentation: 仙台
• [Presentation] Compensatory Lung Growth Triggered by Major Lung Resection Determined by a Novel Method : Estimated Quantity of Pulmonary Tissue (EQPT) (2011)
  • Author(s): Mizobuchi T, Inage T, Morimoto J, Sakairi Y, Ishibashi F, Chiyo M, Kometani T, Iwata T, Moriya Y, Hoshino H, Yoshida S, Yoshino I
  • Organizer: The annual meeting of American Thoracic Society
  • Place of Presentation: San Francisco, USA
• [Presentation] I型肺胞上皮細胞を用いた肺再生療法の開発代償性肺成長モデルによる基礎実験 (2011)
  • Author(s): 和田啓伸, 吉田成利, 鈴木秀海, 坂入祐一, 石橋史博, 田村創, 岩田剛和, 溝渕輝明, 守屋康光, 星野英久, 岡本龍郎, 本橋新一郎, 河村大輔, 横井左奈, 吉野一郎
  • Organizer: 第47回日本移植学会
  • Place of Presentation: 仙台
• [Presentation] ヒト手術肺からの肺再生療法を目的としたcell sourceの分離および解析 (2011)
  • Author(s): 坂入祐一, 和田啓伸, 鈴木秀海, 吉田成利, 石橋史博, 田村創, 岩田剛和, 溝渕輝明, 守屋康光, 星野英久, 岡本龍郎, 本橋新一郎, 吉野一郎
  • Organizer: 第64回日本胸部外科学会学術集会
  • Place of Presentation: 名古屋市
• [Presentation] Compensatory Lung Growth Triggered by Major Lung Resection Determined by a Novel Method : Estimated Quantity of Pulmonary Tissue (EQPT) (2011)
  • Author(s): Mizobuchi T, Inage T, Morimoto J, Sakairi Y, Ishibashi F, Chiyo M, Kometani T, Iwata T, Moriya Y, Hoshino H, Yoshida S, Yoshino I
  • Organizer: The annual meeting of American Thoracic Society, San Francisco
  • Place of Presentation: USAサンフランシスコ
• [Presentation] 全摘術後残存肺における肺再生にはII型肺胞上皮細胞の補充が必要である
  • Author(s): 和田啓伸, 吉田成利, 鈴木秀海, 坂入祐一, 石橋史博, 田村創, 岩田剛和, 溝渕輝明, 守屋康充, 星野英久, 岡本龍郎, 本橋新一郎, 河村大輔, 横井左奈, 吉野一郎
  • Organizer: 第111回日本外科学会学術集会