Detection of gastrointestinal cancer biomarkers by next-generation mass spectrometry and comprehensive gene expression analysis
| Project/Area Number |
23390320
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University (2013)
University of Toyama (2011-2012) |
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Principal Investigator |
SHIMADA Yutaka 京都大学, 薬学研究科(研究院), 准教授 (30216072)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKADA Kazuhiro 富山大学, 大学院・医学薬学研究部, 教授 (90171967)
OKUMURA Tomoyuki 富山大学, 大学院・医学薬学研究部, 助教 (10533523)
ARIMA Miwako 埼玉県立がんセンター, 臨床腫瘍研究所, 副部長 (00312950)
ABE Tetsuya 愛知県がんセンター, 研究所, 医長 (90378092)
MONMA Kumiko 東京都立駒込病院, 内視鏡科部長 (70509471)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUJIMOTO Gozo 京都大学, 薬学研究科(研究院), 教授 (80172013)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2011: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | バイオマーカー / 食道癌 / miRNA / 早期発見 / 質量分析 / 次世代質量顕微鏡 |
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| Research Abstract |
We here evaluated a next-generation mass spectrometer and found that it not suitable for a comprehensive protein analysis of blood. Therefore, we performed a microRNA analysis of blood. To exclude individual differences between patients, we focused on esophageal mucosal cancer patients who were scheduled to undergo endoscopic mucosal resection (EMR) in the present study. Forty-three patients were recruited from 3 institutes. We analyzed the expression of miRNAs in pre-treatment as well as post-treatment blood samples that were collected from 31 patients. Significant differences were observed in the expression of 11 miRNAs between pre-treatment and post-treatment samples. We will analyze these miRNAs in 30 patients with advanced esophageal cancer in a future study and evaluate their efficacy as biomarkers of esophageal cancer.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Critical role for the receptor tyrosine kinase EPHB4 in esophageal cancers2012
Author(s)
Hasina R, Mollberg N, Kawada I, Mutreja K, Kanade G, Y ala S, Surati M, Liu R, Li X, Zhou Y, Ferguson BD, Nallasura V, Cohen KS, Hyjek E, Mueller J, Kanteti R, El Hashani E, Kane D, Shimada Y, Lingen MW, Husain AN, Posner MC, Waxman I, Villaflor VM, Ferguson MK, V articovski L, V okes EE, Gill P, Salgia R
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Journal Title
Cancer Res
Volume: 73
Pages: 184-194
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[Journal Article] Expression Analysis of iPS Cell Inductive Genes in Esophageal Squamous Cell Carcinoma by Tissue Microarray2012
Author(s)
Shimada Y, Okumura T, Sekine S, Moriyama M, Sawada S, Matsui K, Y oshioka I, Hojo S, Y oshida T, Nagata T, Fukuoka J, Tsukada K
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Journal Title
Anticancer Res
Volume: 32
Pages: 5507-5514
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[Journal Article] Polo-like Kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer2011
Author(s)
Ito T, Sato F, Kan T, Cheng Y, David S, Agarwal R, Paun BC, Jin Z, Olaru A V, Hamilton JP, Selaru FM, Y ang J, Matsumura N, Shimizu K, Abraham JM, Shimada Y, Mori Y, Meltzer SJ
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Journal Title
Int J Cancer
Volume: 129
Pages: 2134-2146
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[Presentation] Newly established Barrett' s adenocarcinoma cell line (TYAE-1)2013
Author(s)
Shimada Y, Moriyama M, Okumura T, Kekine S, Sawada S, Matsui K, Hojo S, Shibuya K, Y oshioka I, Y oshida T , Nagata T , Tsukada T
Organizer
Annual meeting of American Association for Cancer Research
Place of Presentation
Washington DC
Year and Date
2013-04-09
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[Presentation] Identification of microRNAs which regulate stem cell property in esophageal squamous cell carcinoma2012
Author(s)
Okumura T , Shimada Y, Sekine S, Moriyama M, Omura T , Kamiyama K, Hori R, Watanabe T , Hojo S, Matsui K, Y oshioka I, Sawada S, Y oshida T , Nagata T , Tsukada K
Organizer
The 2012 ISDE congress
Place of Presentation
Venice (Italy)
Year and Date
2012-10-16
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[Presentation] Identification of microRNAs which are differentially expressed in p75NTR-positive esophageal squamous cell carcinoma2011
Author(s)
Okumura T , Shimada Y, Sekine S, Moriyama M, Omura T , Kamiyama K, Hori R, Watanabe T , Hojo S, Matsui K, Y oshioka I, Sawada S, Y oshida T , Nagata T , Tsukada K
Organizer
International surgical week 2011
Place of Presentation
Yokohama
Year and Date
2011-08-30
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