Molecular analysis of colorectal cancer with LINE-1 hypomethylation and its application to cancer diagnosis and therapy
Project/Area Number |
23390321
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Tokyo Women's Medical University (2013) Kanazawa University (2011-2012) |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
MINAMOTO Toshinari 金沢大学, がん研究所, 教授 (50239323)
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Co-Investigator(Renkei-kenkyūsha) |
SOGA Tomoyoshi 慶應義塾大学, 環境情報学部, 教授 (60338217)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
|
Keywords | 大腸がん / レトロポゾン / メチル化 / 抗がん剤 / LINE-1 / DNAメチル化 / 5-FU |
Research Abstract |
We investigated the mechanism by which 5-FU is effective on the tumor with LINE-1 hypomethylation. LINE-1 expression status is not associated with tumor sensitivity to 5-FU. Chromatin structure looses in the tumor with LINE-1 hypomethylation. Colorectal cancer cell with loosed chromatin is more sensitive to 5-FU compared to one with condensed chromatin. The results suggest that the association between LINE-1 hypomethylation and chromatin structure underlies mechanism of anticancer activity of 5-FU. Knockdown of LINE-1 expression results in suppression of cancer growth. Many anticancer drugs inhibit LINE-1 expression, suggesting that suppression of LINE-1 might be one of mechanism by which 5-FU and other anticancer drugs shows synergistic effect. These findings could be important for achieving personalized chemotherapy and developing novel strategy using 5-FU.
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Report
(4 results)
Research Products
(20 results)
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[Presentation] Isolation and molecular profiling of a circulating tumor cell by using a novel single cell picking system2013
Author(s)
Kondo Y, Hayashi H, Watabe T, Miwa Y, Kinoshita S, Higashimoto H, Sugisaki H, Nakajima G, Kawakami K, Hayashi K, Yamamoto M
Organizer
9th International Symposium on Minimal Residual Cancer
Place of Presentation
Pullman Paris Bercy (France)
Year and Date
2013-09-25
Related Report
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