Comprehensive proteome analysis of pancreatic cancers for understanding the mechanisms of carcinogenesis and cancer metastasis
| Project/Area Number |
23390326
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | National Center of Neurology and Psychiatry (2012-2013)
Kagawa University (2011) |
|---|
Principal Investigator |
YACHIDA Shinichi 独立行政法人国立がん研究センター, 研究所, ユニット長 (20359920)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
IACOBUZIO-DONAHUE Ca Memorial Sloan Kettering Cancer Center, 病理学, 教授
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
|
|---|
| Keywords | 膵臓癌 / プロテオーム / プロテオーム解析 / 転移 / プロテーム解析 / バイオマーカー |
|---|
| Research Abstract |
Many patients with pancreatic cancer have metastases to distant organs at the time of initial presentation. The goal of this study is to understand the mechanisms of pancreatic cancer metastasis at the proteome level, contributing to create a novel biomarker. To address, we employed a model system in which cells isolated from three sites of metastasis (liver, lung and peritoneum) from a single patient were compared. We used a SILAC-based accurate quantitative proteomic strategy combined with a high resolution mass spectrometry to analyze the total proteome and tyrosine phosphoproteome of each of the metastases. Our data revealed distinct patterns of both overall proteome expression as well as tyrosine kinase activities across the three different metastatic lesions. This heterogeneity is significant because it led to differential sensitivity of the neoplastic cells to small molecule inhibitors targeting various kinases and other pathways.
|
Report
(4 results)
Research Products
(9 results)
-
[Journal Article] Heterogeneity of pancreatic cancer metastases in a single patient revealed by quantitative proteomics2014
Author(s)
Kim MS, Zhong Y, Y achida S, Rajeshkumar NV, Abel ML, Marimuthu A, Mudgal K, Hruban RH, Poling JS, Tyner JW, Maitra A, Iacobuzio-Donahue CA, Pandey A
-
Journal Title
Mol Cell Proteomics
Volume: (in press)
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Clinical significance of the genetic landscape of pancreatic cancer and implications for identification of potential long-term survivors2012
Author(s)
Yachida S, White CM, Naito Y, Zhong Y, Brosnan JA, Macgregor-Das AM, Morgan RA, Saunders T, Laheru DA, Herman JM, Hruban RH, Klein AP, Jones S, V elculescu V, Wolfganag CL, Iacobuzio-Donahue CA
-
Journal Title
Clin Cancer Res
Volume: 18
Issue: 22
Pages: 6339-47
DOI
Related Report
Peer Reviewed
-
-
-
