|Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|Outline of Final Research Achievements
This study investigated the growth-signaling of gastric cancer (GC) cells in focus on the interaction with cancer-associated fibroblasts (CAFs) and gastric cancer (GC) cells under normoxia and hypoxia. Nine cell lines, including four diffuse-type GC cell lines, two intestinal-type GC cell lines, and three CAF cell lines were used. Cells were examined for expression CXCR4, FGFR2 and SDF1. CXCR4 expression by diffuse-type GC cells was significantly increased in hypoxia, while FGFR2 expression was decreased. FGFR2 inhibition significantly decreased the activity of CAFs for diffuse-type GC cells in normoxia but not in hypoxia. In contrast, CXCR4 inhibition significantly decreased the activity of CAFs in hypoxia. HIF1 siRNA significantly decreased both CXCR4 expression by diffuse-type GC cells and SDF1 production by CAFs. These findings suggest that diffuse-type GC cells might switch their driver pathways from FGFR2 signaling to SDF1/CXCR4 axis through HIF1 in hypoxic microenvironments.