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Novel therapeutic strategies using autologous bone marrow-derived stem cells for vascular regeneration

Research Project

Project/Area Number 23390336
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionYamaguchi University

Principal Investigator

HAMANO Kimikazu  山口大学, 医学(系)研究科(研究院), 教授 (60263787)

Co-Investigator(Kenkyū-buntansha) MORIKAGE Noriyasu  山口大学, 医学部附属病院, 講師 (50335741)
LI Tao-sheng  長崎大学, 原爆後障害医療研究所, 教授 (50379997)
KUBO Masayuki  岡山大学, 医歯薬学総合研究科, 助教 (60420519)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords自己細胞移植 / 血管障害 / 末梢血由来幹細胞 / 低酸素プレコンディショニング / 下肢虚血 / 血管再生治療 / 骨髄由来細胞 / 再生医療
Research Abstract

Autologous transplantation of stem cells often shows limited clinical outcomes, and a discrepancy of therapeutic effects between patients have been reported. Based on our previsous studies demonstrating that hypoxic pre-treatment reinforces cellular functions in small animal experiments, we planned to appply hypoxic pre-treatment protocol to patients with vascular disease. In this study, we examined wether autologous transplantation of peripheral blood-derived stem cells (PBSCs) which have preconditioned in hypoxic incubation accelerates anigogenesis in the hypixic tissues. Autologous transplantation of preconditioned-PBSCs resulted in an acceleration of angiogenesis and an improvement of blood flow in the hypoxia-induced hindlimb of middle animals. We also demonstrated that hypoxic pretreatment induces reinforcement of cellular functions in human PBSCs. Taken together, hypoxic pretreatment of stem cells is promising therapeutic approach for human clinical trials.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (21 results)

All 2014 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (15 results)

  • [Journal Article] Hypoxic preconditioning reinforces cellular functions of autologous peripheral blood-derived cells in rabbit hindlimb ischemia model2014

    • Author(s)
      Kudo T, Hosoyama T, Samura M, Katsura S, Nishimoto A, Kugimiya N, Fujii Y, Li TS, Hamano K
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 444 Pages: 370-375

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hypoxic preconditioning reinforces cellular functions of autologous peripheral blood-derived cells in rabbit hindlimb ischemia model.2014

    • Author(s)
      Kudo T, Hosoyama T, Samura M, Katsura S, Nishimoto A, Kugimiya N, Fujii Y, Li TS, Hamano K.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 444 Issue: 3 Pages: 370-375

    • DOI

      10.1016/j.bbrc.2014.01.054

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heat shock factor 1 contributes to ischemia-induced angiogenesis by regulating the mobilization and recruitment of bone marrow stem/progenitor cells2012

    • Author(s)
      Kubo M, Li TS, Kurazumi H, Takemoto Y, Ohshima M, Yamamoto Y, Nishimoto A, Mikamo A, Fujimoto M, Nakai A, Hamano K
    • Journal Title

      PLoS One

      Volume: 7

    • NAID

      120006985399

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hypoxic preconditioning enhances angiogenic potential of bone marrow cells with aging-related functional impairment2012

    • Author(s)
      Kubo M, Li TS, Kurazumi H, Takemoto Y, Ohshima M, Murata T, Katsura S, Morikage N, Furutani A, Hamano K
    • Journal Title

      Circ J

      Volume: 76 Pages: 986-994

    • NAID

      10030131630

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hypoxic preconditioning enhances angiogenic potential of bone marrow cells with aging-related functional impairment2012

    • Author(s)
      Masayuki Kubo
    • Journal Title

      Circ J

      Volume: (in press)

    • NAID

      10030131630

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diabetic impairment of C-kit bone marrow stem cells involves the disorders of inflammatory factors, cell adhesion and extracellular matrix molecules2011

    • Author(s)
      Li TS, Ikeda S, Kubo M, Ohshima M, Kurazumi H, Takemoto Y, Ueda K, Hamano K
    • Journal Title

      PLoS One

      Volume: 6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Mechanical stress/hemodynamic unloading affects the growth, release of paracrine factors, and differentiations of Cardiosphere-derived cells2013

    • Author(s)
      Hiroshi Kurazumi, Tao-Sheng Li, Kimikazu Hamano
    • Organizer
      Cell Symposia
    • Place of Presentation
      米国カリフォルニア州ロサンゼルス市
    • Year and Date
      2013-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 自己末梢血単核球を用いた血管新生療法における低酸素プレコンディショニングの前臨床試験2013

    • Author(s)
      工藤 智明, 細山 徹, 桂 春作, 弘中紫, 大島 真子, 久保 正幸, 李 桃生, 濱野 公一
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜, パシフィコ横浜
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 自己末梢血単核球を用いた血管新生療法における低酸素プレコンディショニングの前臨床試験2013

    • Author(s)
      工藤智明
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Related Report
      2012 Annual Research Report
  • [Presentation] メカニカルストレスが細胞移植による心筋再生療法に対して及ぼす影響2012

    • Author(s)
      藏澄 宏之, 李 桃生, 山本 由美, 西本 新, 久保 正幸, 濱野 公一
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      横浜, パシフィコ横浜
    • Year and Date
      2012-06-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 心臓が発生するメカニカルストレスに着目した心筋再生療法に関する研究2012

    • Author(s)
      藏澄 宏之, 李 桃生, 池永 茂, 白澤文吾, 美甘 章仁, 濱野 公一
    • Organizer
      第11回再生心臓血管外科治療研究会
    • Place of Presentation
      秋田, 秋田キャッスルホテル
    • Year and Date
      2012-04-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Mechanical stress supresses the proliferation and improves the differentiation of cardiac stem cells and increases the release of paracrine factors2012

    • Author(s)
      Hiroshi Kurazumi, Tao-Sheng Li, Masayuki Kubo, Shigeru Ikenaga, Akihito Mikamo, Kimikazu Hamano
    • Organizer
      ASCVTS 20TH ANNUAL MEETING
    • Place of Presentation
      インドネシアバリ島
    • Year and Date
      2012-03-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 熱ショック転写因子HSF1 は骨髄幹細胞の動態制御を介して, 虚血組織での血管新生に関与する2011

    • Author(s)
      久保 正幸, 李 桃生, 美甘 章仁, 藤本 充章, 中井 彰, 濱野 公一
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜, パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Hypoxic preconditioning enhances the angiogenic and therapeutic potential of bone marrow cells with age-related functional impairment2011

    • Author(s)
      Masayuki Kubo
    • Organizer
      American Heart Association Scientific Sessions 2011
    • Place of Presentation
      Orange County convention Centre(オーランド,アメリカ)
    • Year and Date
      2011-11-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Hypoxic preconditioning enhances the angiogenic and therapeutic potential of bone marrow cells with age-related functional impairment2011

    • Author(s)
      Masayuki Kubo, Tao-Sheng Li, Hiroshi Kurazumi, Yoshihiro Takemoto, Mako Ohshima, Kimikazu Hamano
    • Organizer
      American Heart Association Scientific Sessions
    • Place of Presentation
      米国フロリダ州オーランド市
    • Year and Date
      2011-11-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨髄由来細胞を用いた血管再生治療に対する低酸素プレコンディショニング2011

    • Author(s)
      藏澄 宏之, 久保 正幸, 大島 真子, 李 桃生, 濱野 公一
    • Organizer
      第11回心血管再生先端治療フォーラム
    • Place of Presentation
      東京, 品川グランドセントラルタワー
    • Year and Date
      2011-07-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨髄由来細胞を用いた血管再生治療に対する低酸素プレコンディショニング2011

    • Author(s)
      藏澄宏之
    • Organizer
      第11回心血管再生先端治療フォーラム
    • Place of Presentation
      品川グランドセントラルタワー(東京都)
    • Year and Date
      2011-07-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 骨髄幹細胞を用いた血管再生治療に対する低酸素プレコンディショニング2011

    • Author(s)
      藏澄宏之
    • Organizer
      第111回日本外科学会学術総会
    • Place of Presentation
      (紙上開催)(2011.3.11東日本大震災のため)
    • Year and Date
      2011-05-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 低酸素プレコンディショニングによる移植細胞の機能増強に基づいた血管再生治療法の開発2011

    • Author(s)
      久保 正幸, 李 桃生, 藏澄 宏之, 森景 則保, 美甘 章仁, 濱野 公一
    • Organizer
      日本循環器学会第98回中国・四国合同地方会
    • Place of Presentation
      徳島, あわぎんホール
    • Year and Date
      2011-05-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 低酸素プレコンディショニングによる移植細胞の機能増強に基づいた血管再生治療法の開発2011

    • Author(s)
      久保正幸
    • Organizer
      日本循環器学会第98回中国・四国合同地方会
    • Place of Presentation
      あわぎんホール(徳島県)
    • Year and Date
      2011-05-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 自己末梢血単核球を用いた血管新生療法における低酸素プレコンディショニング(HPC)の有用性

    • Author(s)
      工藤智明、細山 徹、佐村 誠、濱野公一
    • Organizer
      第103回日本循環器学会中国地方会
    • Place of Presentation
      鳥取県米子市(米子コンベンションセンター)
    • Related Report
      2013 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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