Development of Novel Biodegradable Scaffold for Non-Cell-Seeding In-vivo Tissue-Engineering Vasculature for Clinical Applications

• Project/Area Number: 23390339
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: MATSUMURA Goki 東京女子医科大学, 医学部, 助教 (20297469)
• Co-Investigator(Kenkyū-buntansha): YAMAZAKI Kenji 東京女子医科大学, 医学部, 教授 (30241087)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 再生医療 / 生分解性素材 / 橋渡し研究 / 再生医工学 / トランスレーショナルリサーチ / 生体吸収性素材 / 心臓血管外科 / ﾃｨｯｼｭｴﾝｼﾞﾆｱﾘﾝｸﾞ / 医療材料 / 先天性心疾患 / 再生血管 / ﾄﾗﾝｽﾚｰｼｮﾅﾙﾘｻｰﾁ / 生体適合性 / ティッシュエンジニアリング

Research Abstract

We have developed a new biodegradable scaffold that does not require any cell preparation steps before its implantation to create an in-situ tissue-engineering vasculature (iTEV). Animal experiments were conducted to test its characteristics and long-term efficacy in a venous and a pulmonary positions. Eight to 10-millimeter tubular-shaped biodegradable scaffolds which consist of polyglycolide knitted fiber and L-lactide and -caprolactone copolymer sponge with outer glycolide and e-caprolactone copolymer monofilament reinforcement were implanted into canine inferior vena cava (IVC) and left pulmonary artery. All canines were well alive until each experiment, and the utility of the iTEV was evaluated from 1 to 24 months according to the studies. Histological studies and angiographies revealed well formed iTEV without marked stenosis/thrombosis or calcifications. These results provide direct evidence that this new scaffold can be useful for cell free tissue-engineering of vasculature.

Research Products

• [Journal Article] Histological maturation of vascular smooth muscle cells in in situ tissue-engineered vasculature (2014)
  • Author(s): Noriko Isayama, Goki Matsumura, Hideki Sato, Shojiro Matsuda, Kenji Yamazaki
  • Journal Title: Biomaterials Volume: 35(1) Pages: 3589-3595
• [Journal Article] Long-term results of cell-free biodegradable scaffolds for in situ tissue engineering of pulmonary artery in a canine model (2013)
  • Author(s): Goki Matsumura, Noriko Isayama, Shojiro Matsuda, Kensuke Taki, Yuki Sakamoto, Yoshito Ikada, Kenji Yamazaki
  • Journal Title: Biomaterials Volume: 34 Pages: 6422-6428
• [Journal Article] Comparison of vascular smooth muscle cells in canine great vessels (2013)
  • Author(s): Noriko Isayama, Goki Matsumura and Kenji Yamazaki
  • Journal Title: BMC Veterinary Research Volume: 9 Pages: 1-8
  • NAID: 120005538080
• [Journal Article] Comparison of Vascular Smooth Muscle Cells in Canine Great Vessels (2013)
  • Author(s): Noriko Isayama
  • Journal Title: BMC Veterinary Research Volume: 9 Issue: 1 Pages: 1-8
  • DOI: 10.1186/1746-6148-9-54
  • Peer Reviewed
• [Journal Article] Long-term results of cell-free biodegradable scaffolds for in situ tissue-engineering vasculature : in a canine inferior vena cava model (2012)
  • Author(s): Matsumura, G., Nitta, N., Matsuda, S., Sakamoto, Y., Isayama, N., Yamazaki, K., Ikada, Y
  • Journal Title: PLoS One Volume: 7, 4
• [Journal Article] Long-term results of cell-free biodegradable scaffolds for in situ tissue-engineering vasculature: in a canine inferior vena cava model (2012)
  • Author(s): Goki Matsumura
  • Journal Title: PLoS One Volume: 7 Issue: 4 Pages: e35760-e35760
  • DOI: 10.1371/journal.pone.0035760
  • Peer Reviewed
• [Journal Article] 細胞を用いない生体吸収性素材のみによる再生血管の作成と肺動脈部位への適応拡大 (2012)
  • Author(s): 松村剛毅
  • Journal Title: 日本心臓血管外科学会誌 Volume: 42
• [Journal Article] 特集「再生医療-幹細胞操作と工学技術からのアプローチ-」 細胞を用いずに自己の血管を再生させる人工血管(i-TEV) (2011)
  • Author(s): 松村剛毅
  • Journal Title: 人工臓器 Volume: 40, 3 Pages: 236-239
• [Journal Article] 細胞を用いずに自己の血管を再生させる人工血管in-situ Tissue-Engineering Vasculature (iTEV) (2011)
  • Author(s): 松村剛毅
  • Journal Title: 人工臓器 Volume: 40 Pages: 236-239
  • NAID: 10030339943
• [Presentation] 臨床応用を目指した細胞を用いない生体吸収性素材のみによる再生血管 (2014)
  • Author(s): 松村剛毅, 諫山紀子, 山崎健二
  • Organizer: 再生心臓血管外科治療研究会
  • Place of Presentation: 熊本
  • Year and Date: 2014-02-19
• [Presentation] Tissue Engineered valves and vessels : Where do we stand today? (2014)
  • Author(s): Goki Matsumura
  • Organizer: The 5th Congress of Asia Pacific Pediatric Cardiac Society; APPCS
  • Place of Presentation: New Delhi, India, Plenary Session
  • Invited
• [Presentation] Tissue Engineered valves and vessels: Where do we stand today? (2014)
  • Author(s): Goki Matsumura
  • Organizer: The 5th Congress of Asia Pacific Pediatric Cardiac Society
  • Place of Presentation: New Delhi, India
  • Invited
• [Presentation] 生体吸収性素材による再生血管療法 (2013)
  • Author(s): 松村剛毅
  • Organizer: 第19回バイオレオロジー・リサーチ・フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2013-12-05
• [Presentation] Symposium Heart7 Regenerative medicine for artificial organs, Development of in-situ Tissue-Engineered Vasculature (iTEV) (2013)
  • Author(s): Matsumura, G, et al
  • Organizer: JSAO / IFAO
  • Place of Presentation: Yokohama
  • Year and Date: 2013-09-27
• [Presentation] Development of in-situ Tissue-Engineered Vasculature (iTEV) (2013)
  • Author(s): Goki Matsumura, Noriko Isayama, Kenji Yamazaki
  • Organizer: JSAO /IFAO joint symposium
  • Place of Presentation: Yokohama
  • Invited
• [Presentation] 細胞を用いない生体吸収性素材のみによる再生血管の作成と肺動脈部位への適応拡大 (2012)
  • Author(s): 松村剛毅, 諫山紀子, 山崎健二
  • Organizer: 第42回日本心臓血管外科学会総会
  • Place of Presentation: 秋田
  • Year and Date: 2012-04-18
• [Presentation] Long-Term Results of Cell-Free Biodegradable Scaffolds for In Situ Tissue-Engineering Pulmonary Artery in a Canine Model (2012)
  • Author(s): Goki Matsumura
  • Organizer: AHA 2012, Los Angeles
  • Place of Presentation: Los Angeles, Convention center
• [Presentation] 臨床応用を目指した細胞を用いない生体吸収性素材のみによる再生血管 (2012)
  • Author(s): 松村剛毅
  • Organizer: 第50回日本人工臓器学会大会
  • Place of Presentation: 福岡
• [Presentation] 生体吸収性素材による再生血管の作成とその変遷・現状・そして今後のストラテジー (2011)
  • Author(s): 松村剛毅
  • Organizer: 日本心不全学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2011-10-14
• [Presentation] Tissue Engineering Vasculature using Biodegradable Scaffold : Single Center Experience of In-Vitro and In-Vivo Studies (2011)
  • Author(s): Goki Matsumura
  • Organizer: International Symposium on Regenerative Medicine KIST-Wake Forest-KNUH Joint Symposium
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-07-15
• [Presentation] Tissue Engineering Vasculature using Biodegradable Scaffold : Single Center Experience of In-Vitro and In-Vivo Studies (2011)
  • Author(s): Goki Matsumura
  • Organizer: International Symposium on Regenerative Medicine、Cardiac and Vascular Tissue Engineering
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-07-15
• [Presentation] 自己細胞と生体吸収性ポリマーを用いた心外導管型TCPC 25 例の臨床研究の遠隔成績と今後のストラテジー (2011)
  • Author(s): 松村剛毅, 平松健司, 小沼武司, 大倉正寛, 立石 実, 小嶋 愛, 腰山 宏, 久米悠太, 池田ちひろ, 鈴木健二, 山崎健二
  • Organizer: 第47回日本小児循環器学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2011-07-07
• [Presentation] 自己細胞と生体吸収性ポリマーを用いた心外導管型TCPC25例の臨床研究の遠隔成績と今後のストラテジー (2011)
  • Author(s): 松村剛毅
  • Organizer: 日本小児循環器学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2011-07-06
• [Presentation] 細胞を用いない生体吸収性素材のみによる再生血管の作成とその遠隔成績 (2011)
  • Author(s): 松村剛毅, 松田晶二郎, 坂元悠紀, 新田尚隆, 山崎健二
  • Organizer: 第47回日本小児循環器学会総会・学術集会
  • Place of Presentation: 福岡
• [Presentation] Long-Term Results of Cell-Free Biodegradable Scaffolds for In Situ
  • Author(s): Goki Matsumura, MD, PhD, Shojiro Matsuda, PhD, Yuki Sakamoto, MEng, Noriko Isayama, DVM, Yoshito Ikada, PhD and Kenji Yamazaki, MD, PhD
  • Organizer: Tissue-Engineering Pulmonary Artery in a Canine Model, AHA2012
  • Place of Presentation: Los Angeles
• [Presentation] 生体吸収性素材による再生血管療法
  • Author(s): 松村剛毅
  • Organizer: バイオレオロジー・リサーチ・フォーラム
  • Place of Presentation: 東京
  • Invited
• [Presentation] 臨床応用を目指した細胞を用いない生体吸収性素材のみによる再生血管
  • Author(s): 松村剛毅、諫山紀子、山崎健二
  • Organizer: 再生心臓血管外科治療研究会
  • Place of Presentation: 熊本