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Development of new anti-tumor therapy for malignant glioma using a drug incorporating polymeric micelle attached with a specific antibody for glioma cancer stem cell

Research Project

Project/Area Number 23390351
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionKumamoto University

Principal Investigator

KURATSU Jun-ichi  熊本大学, 大学院生命科学研究部, 教授 (20145296)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Hideo  熊本大学, 医学部附属病院, 講師 (30359963)
MAKINO Keishi  熊本大学, 医学部附属病院, 講師 (90381011)
HIDE Takuichiro  熊本大学, 医学部附属病院, 助教 (40421820)
ARAKI Norie  熊本大学, 大学院・生命科学研究部, 准教授 (80253722)
KURODA Jun-ichiro  熊本大学, 大学院・生命科学研究部, 助教 (90536731)
松村 保宏  独立行政法人国立がんセンター, がん治療開発部, 部長 (90209619)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywordsグリオーマ癌幹細胞(GSC) / 薬剤内包ミセル / GSC特異的細胞表面分子 / ヌードマウス / テモゾロマイド / 分子標的薬剤 / グリオーマ / 幹細胞 / 薬物内包ミセル / 抗体付加ミセル / プロテオミクス / GSC特異的抗体 / GSC薬剤耐性 / 幹細胞樹立 / GSC移植 / 動物実験モデル / ラジオアイソトープ
Research Abstract

We developed the method for concentrating and enriching Glioma stem cell(GSC) by subcutaneous implantation of malignant glioma cells into nude mice. By this method we could obtained tumor cells including abundant GSCs. We have been identifying a new molecule specific for GSC by some molecular biological techniques, however, we have not yet found the most suitable molecule. In parallel, we mixed temozolomide and fibrin glue and investigated the anti-tumor effect of this drug in the mouse model.
We found the efficacy of this drug and we think we should search for an optimal molecular target drug.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (33 results)

All 2013 2012 2011 Other

All Journal Article (13 results) (of which Peer Reviewed: 11 results) Presentation (18 results) (of which Invited: 2 results) Remarks (2 results)

  • [Journal Article] New Treatment Strategies to Eradicate Cancer Stem Cells and Niches in Glioblastoma2013

    • Author(s)
      Takuichiro Hide, Keishi Makino, Hideo Nakamura, Jun-ichiro Kuroda, Jun-ichi Kuratsu(他5名)
    • Journal Title

      Neurologia medico-chirurgica

      Volume: 53 Issue: 11 Pages: 764-772

    • DOI

      10.2176/nmc.ra2013-0207

    • NAID

      10031196787

    • ISSN
      0470-8105, 1349-8029
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prognostic value of isocitrate dehydrogenase 1, O6-methylguanine-DNA methyltransferase promoter methylation, and 1p19q co-deletion in Japanese Malignant glioma patients2013

    • Author(s)
      Yoshinobu Takahashi, Hideo Nakamura, Keishi Makino, Takuichiro Hide, Jun-ichi Kuratsu(他2名)
    • Journal Title

      World Journal of Surgical Oncology

      Volume: 11 Issue: 1 Pages: 284-284

    • DOI

      10.1186/1477-7819-11-284

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] TGF-β mediates homing of bone marrow -derived human mesenchymal stem cells to glioma stem cells2013

    • Author(s)
      Naoki Shinojima, Tatsuya Takezaki, Jun-ichi Kuratsu(他8 名)
    • Journal Title

      Cancer Research

      Volume: 73(7) Issue: 7 Pages: 2333-2344

    • DOI

      10.1158/0008-5472.can-12-3086

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Randomized trial of chemoradiotherapy and adjuvant chemotherapy with nimustine versus nimustine plus procarbazine for newly diagnosed anaplastic astrocytoma and glioblastoma .2013

    • Author(s)
      Shibui S, Narita Y, Mizusawa J, Beppu T, Ogasawara K, Sawamura Y, Kobayashi H, Nishikawa R, Mishima K, Muragaki Y, Maruyama T, Kuratsu J, Nomura K etl .
    • Journal Title

      Cancer Chemother Pharmacol.

      Volume: 71(2) Issue: 2 Pages: 511-21

    • DOI

      10.1007/s00280-012-2041-5

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 特集 : 神経疾患の新しい治療- 現場で必須の知識と今後の展望- 脳腫瘍2013

    • Author(s)
      中村英夫, 倉津純一
    • Journal Title

      日本内科学会雑誌

      Volume: 102(8) Pages: 1952-1957

    • URL

      https://www.jstage.jst.go.jp/browse/naika/-char/ja

    • Related Report
      2013 Final Research Report
  • [Journal Article] 特集: 神経疾患の新しい治療 -現場で必須の知識と今後の展望- 脳腫瘍2013

    • Author(s)
      中村英夫、倉津純一
    • Journal Title

      日本内科学会雑誌

      Volume: 102(8) Pages: 1952-1957

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Fatty acid synthase is a predictive marker for aggressiveness in meningiomas.2012

    • Author(s)
      Makino K, Nakamura H, Hide T, Yano S, Kuroda J, Iyama K, Kuratsu J.
    • Journal Title

      J Neurooncol

      Volume: 109(2) Issue: 2 Pages: 399-404

    • DOI

      10.1007/s11060-012-0907-3

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of autophagic cell death of glioma-initiating cells by cell-penetrating d-isomer peptides consisting of Pas and the p53 C-terminus.2012

    • Author(s)
      Ueda Y
    • Journal Title

      Biomaterials.

      Volume: 33 Issue: 35 Pages: 9061-9069

    • DOI

      10.1016/j.biomaterials.2012.09.003

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of neoadjuvant therapy in patients with nongerminomatous malignant germ cell tumors2011

    • Author(s)
      Nakamura H., et al
    • Journal Title

      Journal of Neurosurgery. Pediatrics

      Volume: 7 Issue: 4 Pages: 431-438

    • DOI

      10.3171/2011.1.peds10433

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Therapy-associated secondary tumors in patients with non-germinomatous malignant germ cell tumors2011

    • Author(s)
      Hideo Nakamura
    • Journal Title

      Journal of Neurooncology

      Volume: 105 Issue: 2 Pages: 359-364

    • DOI

      10.1007/s11060-011-0597-2

    • NAID

      120005199486

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kumamoto Brain Tumor Group : Risk of primary childhood brain tumors related to season of birth in Kumamoto Prefecture2011

    • Author(s)
      Keishi Makino, Hideo Nakamura, Takuichiro Hide, Jun-ichi Kuratsu
    • Journal Title

      Japan. Child's Nervous System

      Volume: 27(1) Issue: 1 Pages: 75-78

    • DOI

      10.1007/s00381-010-1235-6

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Does adding FDG-PET to MRI improve the differentiation between primary cerebral lymphoma and glioblastoma? Observer performance study2011

    • Author(s)
      Makino K., et al
    • Journal Title

      Annals of nuclear medicine

      Volume: 25 Issue: 6 Pages: 432-438

    • DOI

      10.1007/s12149-011-0483-1

    • NAID

      10029284684

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of neoadjuvant therapy in patients with nongerminomatous malignant germ cell tumors2011

    • Author(s)
      Hideo Nakamura
    • Journal Title

      Journal of Neurosurgery : Pediatrics

      Volume: 7 Pages: 431-438

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 悪性神経膠腫の遺伝子染色体異常と臨床的予後との相関解析2013

    • Author(s)
      中村英夫
    • Organizer
      日本脳神経外科学会第72回学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2013-10-17
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Genetic alterations in glioma2013

    • Author(s)
      Hideo Nakamura
    • Organizer
      15th WFNS World Congress of Neurosurgery
    • Place of Presentation
      Coex Convention Center(Seoul, Korea)
    • Year and Date
      2013-09-09
    • Related Report
      2013 Final Research Report
  • [Presentation] Grade IIIグリオーマにおける遺伝子変化と臨床的予後との関係2012

    • Author(s)
      中村英夫
    • Organizer
      第30回日本脳腫瘍学会学術集会
    • Place of Presentation
      グランドプリンス広島(広島県広島市)
    • Year and Date
      2012-11-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic alterations in glioma by using SNIP-Microarray2012

    • Author(s)
      Hideo Nakamura
    • Organizer
      The 10th Meeting of Europian Association of Neuro-oncology
    • Place of Presentation
      PARC CHANOT Convention & Exhibition Center (Marseille, France)
    • Year and Date
      2012-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 頭蓋内非腫瘍性病変の鑑別診断における変異型IDH1抗体を用いた免疫組織染色の有用性の検討2012

    • Author(s)
      牧野敬史
    • Organizer
      第30回日本脳腫瘍病理学会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2012-05-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Usefulness of immunohistochemistry using mutant IDH1 antibody to differentiate intracranial nonneoplastic lesions2012

    • Author(s)
      Keishi Makino
    • Organizer
      9th Meeting of Asian Society for Neuro-oncology
    • Place of Presentation
      Taipei Internation Convention Center(Taipei, Taiwan)
    • Year and Date
      2012-04-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic alterations in glioma by using SNIP-Microarray2012

    • Author(s)
      Hideo Nakamura
    • Organizer
      The 10th Meeting of Europian Association of Neuro-oncology
    • Place of Presentation
      Marseille, France
    • Related Report
      2012 Annual Research Report
  • [Presentation] Grade IIIグリオーマにおける遺伝子変化と臨床的予後との関係2012

    • Author(s)
      中村英夫
    • Organizer
      第30回日本脳腫瘍学会
    • Place of Presentation
      グランドプリンスホテル広島
    • Related Report
      2012 Annual Research Report
  • [Presentation] Usefulness of immunohistochemistry using mutant IDH1 antibody to differentiate intracranial nonneoplastic lesions2012

    • Author(s)
      Makino K
    • Organizer
      9th Meeting of Asian Society for Neuro-Oncology
    • Place of Presentation
      Taipei, Taiwan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 頭蓋内非腫瘍性病変の鑑別診断における変異型IDH1抗体を用いた免疫組織染色の有用性の検討2012

    • Author(s)
      牧野敬史
    • Organizer
      第30回日本脳腫瘍病理学会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] オリゴデンドロサイト前駆細胞の形質転換に伴う遺伝子発現の変化2011

    • Author(s)
      秀 拓一郎
    • Organizer
      第29回日本脳腫瘍学会学術総会
    • Place of Presentation
      水明館(岐阜県下呂市)
    • Year and Date
      2011-11-28
    • Related Report
      2013 Final Research Report
  • [Presentation] オリゴデンドロサイト前駆細胞の形質転換に伴う遺伝子発現の変化2011

    • Author(s)
      秀拓一郎
    • Organizer
      第29回日本脳腫瘍学会学術総会
    • Place of Presentation
      水明館、岐阜県下呂温泉
    • Year and Date
      2011-11-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 悪性脳腫瘍における fatty acid synthase の発現とその意義2011

    • Author(s)
      牧野敬史
    • Organizer
      日本脳神経外科学会第70回学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2011-10-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 悪性脳腫瘍におけるfatty acid synthaseの発現とその意義2011

    • Author(s)
      牧野敬史
    • Organizer
      第70回日本脳神経外科学会総会
    • Place of Presentation
      パシフィコ横浜、横浜
    • Year and Date
      2011-10-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular allelokaryotyping of malignant glioma by high-resolution single nucleotide polymorphism(SNP) oligonucleotide genomic microarray2011

    • Author(s)
      Hideo Nakamura
    • Organizer
      International Symposium on Clinical and Basic Investigation in Glioblastoma/ II Symposium Seve Ballesteros Foundation
    • Place of Presentation
      Santiago Grisolía Auditorium(Valencia, Spain)
    • Year and Date
      2011-06-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular allelokaryotyping of malignant glioma by high-resolution single nucleotide polymorphism (SNP) oligonucleotide genomic microarray2011

    • Author(s)
      Hideo Nakamura
    • Organizer
      International Symposium on Clinical and Basic Investigation in Glioblastoma/II Symposium Seve Ballesteros Foundation
    • Place of Presentation
      Santiago Grisolia Auditorium, Valencia Spain
    • Year and Date
      2011-06-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Genetic alterations in glioma.

    • Author(s)
      Nakamura H
    • Organizer
      15th WFNS World Congress of Neurosurgery
    • Place of Presentation
      Coex Convention Center(Seoul, Korea)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 悪性神経膠腫の遺伝子染色体異常と臨床的予後との相関解析

    • Author(s)
      中村英夫
    • Organizer
      日本脳神経外科学会 第72回学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2013 Annual Research Report
  • [Remarks] 熊本大学医学部附属病院 脳神経外科ホームページ

    • URL

      http://www2.kuh.kumamoto-u.ac.jp/neurosurgery/

    • Related Report
      2013 Final Research Report
  • [Remarks] 熊本大学医学部附属病院 脳神経外科ホームページ

    • URL

      http://www2.kuh.kumamoto-u.ac.jp/neurosurgery/

    • Related Report
      2013 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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