Treatment strategy and analysis of progression mechanism of musculoskeletal tumors using fluorescent imaging

• Project/Area Number: 23390360
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kanazawa University
• Principal Investigator: TSUCHIYA Hiroyuki 金沢大学, 医学系, 教授 (40227434)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Norio 金沢大学, 医薬保健学総合研究科, 特任教授 (90332668) ; SHIRAI Toshiharu 京都府立医科大学, 大学院・医学研究科, 講師 (20397186) ; HAYASHI Katsuhiro 金沢大学, 大学病院, 助教 (80507054)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 骨・軟部腫瘍学 / 癌 / 蛍光イメージング

Research Abstract

Fluorescent labeled osteosarcoma cell line was transplanted in nude mice and harvested in lung metastasis to obtain higher metastatic cell line. The more metastatic potential, the more PAI -1 and u-PA levels increased. We previously reported that caffeine-assisted chemotherapy. We found caffeine affects tumor cells through various pathways, such as PTEN, AKT, NF-kB, and ERK activities. Modulation of cisplatinum efficacy induced by caffeine was visualized at the subcellular level by fluorescent ubiquitination-based cell cycle indicator in the nucleus. Caffeine overcame the cell-cycle arrest induced by cisplatinum, thereby increasing its efficacy. Theobromine, a caffeine derivative, was investigated as a new anti-cancer effect. It elevates intracellular cAMP levels and inhibits cell proliferation. Surgical navigation for bone and soft tissue tumor in mouse and human was also performed using ICG and near-infrared fluorescent imaging.

Research Products

• [Journal Article] Theobromine, the primary methylxanthine found in Theobroma cacao, prevents malignant glioblastoma proliferation by negatively regulating phosphodiesterase-4, extracellular signal-regulated kinase, Akt/mammalian target of rapamycin kinase, and nuclear factor-kappa B (2014)
  • Author(s): Sugimoto N, Miwa S, Hitomi Y, Nakamura H, Tsuchiya H, Yachie A
  • Journal Title: Nutr Cancer Volume: 66(3) Issue: 3 Pages: 419-23
  • DOI: 10.1080/01635581.2013.877497
  • Peer Reviewed
• [Journal Article] A novel method to apply osteogenic potential of adipose derived stem cells in orthopaedic surgery (2014)
  • Author(s): Fang X, Murakami H, Demura S, Hayashi K, Matsubara H, Kato S, Yoshioka K, Inoue K, Ota T, Shinmura K, Tsuchiya H
  • Journal Title: PLoS One Volume: 9(2) Issue: 2 Pages: e88874-e88874
  • DOI: 10.1371/journal.pone.0088874
  • Peer Reviewed / Open Access
• [Journal Article] Imaging UVC-induced DNA damage response in models of minimal cancer (2013)
  • Author(s): Miwa S, Yano S, Hiroshima Y, Tome Y, Uehara F, Mii S, Efimova EV, Kimura H, Hayashi K, Tsuchiya H, Hoffman RM
  • Journal Title: J Cell Biochem Volume: 114(11) Issue: 11 Pages: 2493-9
  • DOI: 10.1002/jcb.24599
  • Peer Reviewed
• [Journal Article] Dynamic color-coded fluorescence imaging of the cell-cycle phase, mitosis, and apoptosis demonstrates how caffeine modulates cisplatinum efficacy (2013)
  • Author(s): Miwa S, Yano S, Tome Y, Sugimoto N, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Efimova EV, Fujiwara T, Tsuchiya H, Hoffman RM
  • Journal Title: J Cell Biochem Volume: 114(11) Issue: 11 Pages: 2454-60
  • DOI: 10.1002/jcb.24593
  • Peer Reviewed
• [Journal Article] Single Cell Time-lapse Imaging of Focus Formation by the DNA Damage-Response Protein 53BP1 after UVC Irradiation of Human Pancreatic Cancer Cells (2013)
  • Author(s): Miwa S, Tome Y, Yano S, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Tsuchiya H, Bouvet M, Efimova EV, Hoffman RM
  • Journal Title: Anticancer Res Volume: 33 Pages: 1373-7
  • Peer Reviewed
• [Journal Article] Diagnosis and treatment of low-grade osteosarcoma : experience with nine cases (2013)
  • Author(s): Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Inatani H, Okamoto H, Yamada S, Ikeda H, Sawada-Kitamura S, Nojima T, Ooi A, Otsuka T
  • Journal Title: Int J Clin Oncol Volume: Jul 24 Issue: 4 Pages: 731-8
  • DOI: 10.1007/s10147-013-0592-z
  • Peer Reviewed
• [Journal Article] Pedicle versus free frozen autograft for reconstruction in malignant bone and soft tissue tumors of the lower extremities (2013)
  • Author(s): Shimozaki S, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Kato T, Tsuchiya H
  • Journal Title: J Orthop Sci Volume: 19(1) Issue: 1 Pages: 156-63
  • DOI: 10.1007/s00776-013-0487-x
  • NAID: 120005349170
  • Peer Reviewed
• [Journal Article] Prognostic value of histological response to chemotherapy in osteosarcoma patients receiving tumor-bearing frozen autograft (2013)
  • Author(s): Miwa S, Takeuchi A, Ikeda H, Shirai T, Yamamoto N, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Igarashi K, Tsuchiya H
  • Journal Title: PLoS One Volume: 8(8) Issue: 8 Pages: 71362-71362
  • DOI: 10.1371/journal.pone.0071362
  • Peer Reviewed
• [Journal Article] Chemotherapy for osteosarcoma - where does it come from? What is it? Where is it going? (2013)
  • Author(s): Yamamoto N, Tsuchiya H
  • Journal Title: Expert Opin Pharmacother Volume: 14(16) Issue: 16 Pages: 2183-93
  • DOI: 10.1517/14656566.2013.827171
  • Peer Reviewed
• [Journal Article] Single Cell Time-lapse Imaging of Focus Formation by the DNA Damage-Response Protein 53BP1 after UVC Irradiation of Human Pancreatic Cancer Cells. (2013)
  • Author(s): Miwa S, Tome Y, Yano S, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Tsuchiya H, Bouvet M, Efimova EV, Hoffman RM
  • Journal Title: Anticancer Res. Volume: 33 Pages: 1373-7
  • Peer Reviewed
• [Journal Article] Innovative antimicrobial coating of titanium implants with iodine (2012)
  • Author(s): Tsuchiya H, Shirai T, Nishida H, Murakami H, Kabata T, Yamamoto N, Watanabe K, Nakase J
  • Journal Title: J Orthop Sci Volume: 5 Issue: 5 Pages: 595-604
  • DOI: 10.1007/s00776-012-0247-3
  • NAID: 120004966630
  • Peer Reviewed
• [Journal Article] Treatment strategies for well-differentiated liposarcomas and therapeutic outcomes (2012)
  • Author(s): Yamamoto N, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kitamura S, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 32 Pages: 1821-5
  • NAID: 120004442385
  • Peer Reviewed
• [Journal Article] Histological study on liquid nitrogen-treated tumor-bearing bone of the proximal femur excised eight years after transplantation (2012)
  • Author(s): Yamamoto N, Inatani H, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Shimozaki S, Kato T, Tsuchiya H
  • Journal Title: Low Temp Med Volume: 38 Pages: 5-8
  • Peer Reviewed
• [Journal Article] Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice (2012)
  • Author(s): Kimura H, Tome Y, Momiyama M, Hayashi K, Tsuchiya H, Bouvet M, Hoffman RM
  • Journal Title: Int J Cancer Volume: 131 Issue: 9 Pages: 2027-33
  • DOI: 10.1002/ijc.27475
  • Peer Reviewed
• [Journal Article] A novel combined radiological method for evaluation of the response to chemotherapy for primary bone sarcoma (2012)
  • Author(s): Miwa S, Taki J, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Ooi A, Tsuchiya H
  • Journal Title: Journal of Surgical Oncology Volume: 106 Issue: 3 Pages: 273-9
  • DOI: 10.1002/jso.23074
  • Peer Reviewed
• [Journal Article] Caffeine induces apoptosis by inhibiting Akt/mTOR/S6K, NF-κB and MAPK pathways in osteosarcoma cell (2012)
  • Author(s): Miwa S, Sugimoto N, Yamamoto N, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Yachie A, Tsuchiya H
  • Journal Title: Anticancer Research Volume: 32 Pages: 3643-9
  • Peer Reviewed
• [Journal Article] TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors (2012)
  • Author(s): Miwa S, Nishida H, Tanzawa Y, Takeuchi A, Yamamoto N, Hayashi K, Kimura H, Tsuchiya H
  • Journal Title: PLoS ONE Volume: 926 Issue: 10
  • DOI: 10.1371/annotation/7c0f14e6-cfba-4f5f-b93d-1a149eaeec96
  • Peer Reviewed
• [Journal Article] Uncultured adipose-derived regenerative cells promote peripheral nerve regeneration (2012)
  • Author(s): Suganuma S, Tada K, Hayashi K, Takeuchi A, Sugimoto N, Ikeda K, Tsuchiya H
  • Journal Title: J Orthop Sci Volume: 18 Issue: 1 Pages: 145-51
  • DOI: 10.1007/s00776-012-0306-9
  • Peer Reviewed
• [Journal Article] In vitro and in vivo models for real-time or time course tumor cell imaging (2012)
  • Author(s): Yamamoto N, Tsuchiya H, et al
  • Journal Title: Int J Mol Med Volume: 30
  • Peer Reviewed
• [Journal Article] Giant cell tumor expanded into the thoracic cavity with spinal involvement (2012)
  • Author(s): Demura S, Tsuchiya H, et al
  • Journal Title: Orthopedics Volume: 35 Issue: 3
  • DOI: 10.3928/01477447-20120222-42
  • NAID: 120004140872
  • Peer Reviewed
• [Journal Article] Eosinophilic granuloma of the lumbar spine in an adult (2012)
  • Author(s): Ueda Y, Tsuchiya H, et al
  • Journal Title: Orthopedics Volume: 35 Issue: 12
  • DOI: 10.3928/01477447-20121120-32
  • Peer Reviewed
• [Journal Article] TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors (2012)
  • Author(s): Miwa S, Tsuchiya H, et al
  • Journal Title: PLoS ONE Volume: 7 Issue: 12 Pages: e52926-e52926
  • DOI: 10.1371/journal.pone.0052926
  • Peer Reviewed
• [Journal Article] Basic science and clinical frontiers of sarcoma treatment (2011)
  • Author(s): Tsuchiya H
  • Journal Title: Int J Clin Oncol Volume: 16 Issue: 2 Pages: 82-83
  • DOI: 10.1007/s10147-011-0219-1
  • NAID: 10029366181
  • Peer Reviewed
• [Journal Article] Tumor imaging with multicolor fluorescent protein expression (2011)
  • Author(s): Yamamoto N, Tsuchiya H
  • Journal Title: Int J Clin Oncol Volume: 16 Issue: 2 Pages: 84-91
  • DOI: 10.1007/s10147-011-0201-y
  • NAID: 10029366186
  • Peer Reviewed
• [Journal Article] High-grade undifferentiated pleomorphic sarcoma of pelvis treated with curettage and bone graft after complete remission following caffeine-potentiated chemotherapy (2011)
  • Author(s): Tsuchiya H
  • Journal Title: J Orthop Sci Volume: 16 Issue: 4 Pages: 476-481
  • DOI: 10.1007/s00776-011-0056-0
  • NAID: 10029574174
  • Peer Reviewed
• [Journal Article] Comparison of Cancer-Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-time Imaging in the Live Mouse (2011)
  • Author(s): Hayashi K, Kimura H, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Hasegawa A, Bouvet M, Hoffman RM
  • Journal Title: Anticancer Res Volume: 31 Pages: 3665-72
  • Peer Reviewed
• [Journal Article] Potentiation of the antitumor effect of calcium phosphate cement containing anticancer drug and caffeine on rat osteosarcoma (2011)
  • Author(s): Tanzawa Y, Tsuchiya H, Shirai T, Nishida H, Hayashi K, Takeuchi A, Kawahara M, Tomita K
  • Journal Title: J Orthop Sci Volume: 16 Issue: 1 Pages: 77-84
  • DOI: 10.1007/s00776-011-0045-3
  • NAID: 10029572715
  • Peer Reviewed
• [Journal Article] Use of (99m)Tc-MIBI scintigraphy in the evaluation of the response to chemotherapy for osteosarcoma : comparison with (201)Tl scintigraphy and angiography (2011)
  • Author(s): Miwa S, Shirai T, Taki J, Sumiya H, Nishida H, Hayashi K, Takeuchi A, Ooi A, Tsuchiya H
  • Journal Title: Int J Clin Oncol Volume: 16 Issue: 4 Pages: 373-8
  • DOI: 10.1007/s10147-011-0194-6
  • NAID: 10029527688
  • Peer Reviewed
• [Journal Article] Caffeine activates tumor suppressor PTEN in sarcoma cells (2011)
  • Author(s): Miwa S, Sugimoto N, Shirai T, Hayashi K, Nishida H, Ohnari I, Takeuchi A, Yachie A, Tsuchiya H
  • Journal Title: Int J Oncol Volume: 39 Pages: 465-72
  • DOI: 10.3892/ijo.2011.1051
  • NAID: 120003239459
  • Peer Reviewed
• [Journal Article] Comparison of Cancer-Cell Seeding, Viability and Deformation in the Lung, Muscle and liver, Visualized by Subcellular Real-time Imaging in the Live Mouse (2011)
  • Author(s): Hayashi K, Tsuchiya H, et al
  • Journal Title: Anticancer Res Volume: 31 Pages: 3665-3672
  • Peer Reviewed
• [Journal Article] 【最新基礎科学知っておきたい】がん幹細胞 (2011)
  • Author(s): 武内章彦, 土屋弘行
  • Journal Title: 臨床整形外科 Volume: 46 Pages: 1022-1026
  • Peer Reviewed
• [Journal Article] 小児骨肉腫患者に対するカフェイン併用化学療法の治療成績 (2011)
  • Author(s): 白井寿治, 土屋弘行, 他
  • Journal Title: 日小整会誌 Volume: 20 Pages: 159-163
  • Peer Reviewed
• [Journal Article] 骨肉腫の治療効果判定における画像評価法の検討 (2011)
  • Author(s): 三輪真嗣, 土屋弘行, 他
  • Journal Title: 臨床整形外科 Volume: 46 Pages: 689-695
  • Peer Reviewed
• [Presentation] 骨軟部腫瘍学の進歩 (2013)
  • Author(s): 土屋弘行
  • Organizer: 東日本整形外科災害外科学会
  • Place of Presentation: 軽井沢プリンスホテル(長野)
  • Year and Date: 2013-09-21
  • Invited
• [Presentation] 骨腫瘍における機能再建術 (2013)
  • Author(s): 土屋弘行
  • Organizer: 第9回整形外科アップデートセミナー
  • Place of Presentation: 東京ドームホテル(東京)
  • Year and Date: 2013-07-27
  • Invited
• [Presentation] 骨肉腫に対する挑戦 (2013)
  • Author(s): 土屋弘行
  • Organizer: 平成25年度十全同窓会新任教授就任記念講演会
  • Place of Presentation: 十全講堂(金沢)
  • Year and Date: 2013-07-06
  • Invited
• [Presentation] Biological reconstruction in tumor surgery (2013)
  • Author(s): 土 屋弘行
  • Organizer: Annual Meeting of Hellenic Association of Orthopaedic Surgery and Traumatology
  • Place of Presentation: DIVANI CARAVEL HOTEL (Greece)
  • Invited
• [Presentation] 骨腫瘍 (2013)
  • Author(s): 土屋弘行
  • Organizer: 日本小児整形外科学会
  • Place of Presentation: 京都府立医科大学青蓮会館(京都)
  • Invited
• [Presentation] Invasion and metastasis : from basics to real-time imaging (2013)
  • Author(s): 土屋弘行
  • Organizer: European Musculoskeletal Oncology Society Meeting
  • Place of Presentation: Swedish Exhibition and Congress Center (Sweden)
  • Invited
• [Presentation] 悪性骨軟部腫瘍再建術の未来 (2013)
  • Author(s): 土屋弘行
  • Organizer: 日本整形外科学会学術集会
  • Place of Presentation: リーガロイヤルホテル広島(広島)
  • Invited
• [Presentation] 悪性骨軟部腫瘍再建術の未来 (2013)
  • Author(s): 土屋弘行
  • Organizer: 日本整形外科学会学術集会
  • Place of Presentation: リーガロイヤルホテル広島（広島）
  • Invited
• [Presentation] Invasion and metastasis: from basics to real-time imaging (2013)
  • Author(s): 土屋弘行
  • Organizer: European Musculoskeletal Oncology Society Meeting
  • Place of Presentation: Swedish Exhibition and Congress Center (Sweden)
  • Invited
• [Presentation] 骨肉腫に対する挑戦 (2013)
  • Author(s): 土屋弘行
  • Organizer: 平成２５年度十全同窓会新任教授就任記念講演会
  • Place of Presentation: 十全講堂（金沢）
  • Invited
• [Presentation] 骨腫瘍における機能再建術 (2013)
  • Author(s): 土屋弘行
  • Organizer: 第9回整形外科アップデートセミナー
  • Place of Presentation: 東京ドームホテル(東京）
  • Invited
• [Presentation] 骨腫瘍 (2013)
  • Author(s): 土屋弘行
  • Organizer: 日本小児整形外科学会
  • Place of Presentation: 京都府立医科大学青蓮会館(京都）
  • Invited
• [Presentation] 骨軟部腫瘍学の進歩 (2013)
  • Author(s): 土屋弘行
  • Organizer: 東日本整形外科災害外科学会
  • Place of Presentation: 軽井沢プリンスホテル（長野）
  • Invited
• [Presentation] 骨軟部腫瘍外科医の挑戦 (2013)
  • Author(s): 土屋弘行
  • Organizer: 第20回下関整形外科医会学術講演会
  • Place of Presentation: 下関グランドホテル(山口県）
  • Invited
• [Presentation] Innovative Antimicrobial Titanium Implants Supported By Iodine: A Prospective Multicenter Study. (2013)
  • Author(s): Tsuchiya H
  • Organizer: 2012 Annual Meeting of the American Academy of Orthopaedic Surgeons (AAOS)
  • Place of Presentation: Moscone Center (USA)
  • Invited
• [Presentation] 骨軟部腫瘍学の現状 (2012)
  • Author(s): 土屋弘行
  • Organizer: 第46回東海地区整形外科教育研修会
  • Place of Presentation: ミッドランドホール(名古屋)
  • Year and Date: 2012-10-20
  • Invited
• [Presentation] 骨軟部腫瘍の最先端 (2012)
  • Author(s): 土屋弘行
  • Organizer: 第7回斯整会骨軟部腫瘍検討会特別講演会
  • Place of Presentation: 東急ホテル(名古屋)
  • Year and Date: 2012-08-18
• [Presentation] Progress of osteosarcoma treatment : Ultimate conservative surgery in conjunction with caffeine-potentiated chemotherapy (2012)
  • Author(s): Tsuchiya H
  • Organizer: 17th World Congress on Advances in Oncology and 15th International Symposium on Molecular Medicine
  • Place of Presentation: Creta Maris Hotel (Greece)
  • Invited
• [Presentation] 患肢温存手術の動向-そして未来へ (2012)
  • Author(s): 土屋弘行
  • Organizer: 第119回中部日本整形外科災害外科学会特別講演
  • Place of Presentation: 福井市フェニックスプラザ(福井)
• [Presentation] Update of Caffeine-potentiated chemotherapy for osteosarcoma (2012)
  • Author(s): Tsuchiya H
  • Organizer: The 9th Asia Pacific Muscloskeletal Tumor Society Meeting
  • Place of Presentation: Hilton Kualarunpul (Malaysia)
• [Presentation] Multicenter study of caffeine-potentiated chemotherapy for bone and soft tissue sarcoma (2012)
  • Author(s): Hayashi K, Tsuchiya H, et al
  • Organizer: 9th Asian Pacific Musculoskeletal Tumor Society (APMSTS)
  • Place of Presentation: Hilton Kualarunpul (Malaysia)
• [Presentation] Anti-β1 integrin antibody inhibits osteosarcoma lung seeding and metastasis (2012)
  • Author(s): Kimura H, Tsuchiya H, et al
  • Organizer: 9th Asian Pacific Musculoskeletal Tumor Society (APMSTS)
  • Place of Presentation: Hilton Kualarunpul (Malaysia)
• [Presentation] 腫瘍外科医の限りなき挑戦 (2012)
  • Author(s): 土屋弘行
  • Organizer: 第45回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 東京国際フォーラム(東京)
• [Presentation] Effect of anti-β1 integrin antibody on lung seeding of osteosarcoma cells in live mice visualized by single-cell in vivo imaging (2012)
  • Author(s): Kimura H, Tsuchiya H, et al
  • Organizer: 2012 Annual Meeting of American Society of Clinical Oncology (ASCO)
  • Place of Presentation: McCormick Place (USA)
• [Presentation] ここまで来た骨腫瘍再建術 (2012)
  • Author(s): 土屋弘行
  • Organizer: 第85回日本整形外科学会学術総会
  • Place of Presentation: 国立京都国際会館(京都)
• [Presentation] ここまで来た骨腫瘍再建術 (2012)
  • Author(s): 土屋 弘行
  • Organizer: 第85回 日本整形外科学会学術総会 教育研修講演
  • Place of Presentation: 国立京都国際会館（京都）
  • Invited
• [Presentation] #1. Antimicrobial iodine-coated titanium implants for orthopedic surgery#2. Biological limb reconstruction for tumor surgery#2. Biological limb reconstruction for tumor surgery (2012)
  • Author(s): Tsuchiya H
  • Organizer: 7th ASAMI International Meeting & Bone Reconstruction 2012
  • Place of Presentation: Hyatt Regency Hotel (Greece)
  • Invited
• [Presentation] 腫瘍外科医の限りなき挑戦 (2012)
  • Author(s): 土屋 弘行
  • Organizer: 第45回 日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 東京国際フォーラム（東京）
  • Invited
• [Presentation] 患肢温存手術の動向−そして未来へ (2012)
  • Author(s): 土屋 弘行
  • Organizer: 第119回 中部日本整形外科災害外科学会特別講演
  • Place of Presentation: 福井市フェニックスプラザ（福井）
  • Invited
• [Presentation] Progress of osteosarcoma treatment: Ultimate conservative surgery in conjunction with caffeine-potentiated chemotherapy (2012)
  • Author(s): Tsuchiya H
  • Organizer: 17th World Congress on Advances in Oncology and 15th International Symposium on Molecular Medicine
  • Place of Presentation: Creta Maris Hotel (Greece)
  • Invited
• [Presentation] Joint-preserving reconstruction of metaphyseal malignant bone tumors using tumor-bearing massive bone treated with liquid nitrogen. (2012)
  • Author(s): Tsuchiya H
  • Organizer: 13th European Federation of National Associations of Orthopaedics and Traumatology (EFORT)
  • Place of Presentation: International Congress Centre (Germany)
  • Invited
• [Presentation] Effect of anti-β1 integrin antibody on lung seeding of osteosarcoma cells in live mice visualized by single-cell in vivo imaging. (2012)
  • Author(s): Kimura H, Tsuchiya H, et al
  • Organizer: 2012 Annual Meeting of American Society of Clinical Oncology (ASCO)
  • Place of Presentation: McCormick Place (USA)
• [Presentation] Efficacy of newly developed platinum complexes against osteosarcoma, bone targeting platinum and proteasome inhibitory platinum (2012)
  • Author(s): Igarashi K, Tsuchiya H, et al
  • Organizer: 2012 Annual Meeting of American Society of Clinical Oncology (ASCO)
  • Place of Presentation: McCormick Place (USA)
• [Presentation] Multicenter study of caffeine-potentiated chemotherapy for bone and soft tissue sarcoma. (2012)
  • Author(s): Hayashi K, Tsuchiya H, et al
  • Organizer: 9th Asian Pacific Musculoskeletal Tumor Society (APMSTS)
  • Place of Presentation: Hilton Kualarunpul (Malaysia)
• [Presentation] Anti-β1 integrin antibody inhibits osteosarcoma lung seeding and metastasis. (2012)
  • Author(s): Kimura H, Tsuchiya H, et al
  • Organizer: 9th Asian Pacific Musculoskeletal Tumor Society (APMSTS)
  • Place of Presentation: Hilton Kualarunpul (Malaysia)
• [Presentation] 骨腫瘍の再建術 : 過去・現在・未来 (2011)
  • Author(s): 土屋弘行
  • Organizer: 第7回つくば運動器セミナー
  • Place of Presentation: オークラフロンティアホテルつくば (茨城県)
  • Year and Date: 2011-12-02
• [Presentation] 骨腫瘍の再建術:過去・現在・未来 (2011)
  • Author(s): 土屋弘行
  • Organizer: 第7回つくば運動器セミナー
  • Place of Presentation: オークラフロンティアホテルつくば(茨城県)(招待講演)
  • Year and Date: 2011-12-02
• [Presentation] 抗β1 インテグリン抗体は骨肉腫細胞の肺への接着および肺転移の発生を抑制する (2011)
  • Author(s): 木村浩明, 土屋弘行, 他
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: ベイシア文化ホール(群馬県)
  • Year and Date: 2011-10-21
• [Presentation] 抗β1インテグリン抗体は骨肉腫細胞の肺への接着および肺転移の発生を抑制する (2011)
  • Author(s): 木村浩明、土屋弘行, 他
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: ベイシア文化ホール(群馬県)
  • Year and Date: 2011-10-21
• [Presentation] 肉腫細胞の可視化モデルを用いた肉腫転移機構の解明 (2011)
  • Author(s): 林克洋, 土屋弘行, 他
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: ベイシア文化ホール(群馬県)
  • Year and Date: 2011-10-20
• [Presentation] Real-time imaging of single cancer-cell dynamics of lung metastasis International Society of Limb (2011)
  • Author(s): Kimura H, Tsuchiya H, et al
  • Organizer: Salvage/Musculoskeletal Tumor Society Combined Meeting
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2011-09-18
• [Presentation] Real-time imaging of single cancer-cell dynamics of lung metastasis (2011)
  • Author(s): Kimura H, Tsuchiya H, et al
  • Organizer: International Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2011-09-18
• [Presentation] Benefit of caffeine-potentiated chemotherapy for high-grade soft tissue sarcoma International (2011)
  • Author(s): Takeuchi A, Tsuchiya H, et al
  • Organizer: Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2011-09-17
• [Presentation] Comparison of Sarcoma Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-Time Imaging in the Live Mouse (2011)
  • Author(s): Hayashi K, Tsuchiya H, et al
  • Organizer: International Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2011-09-17
• [Presentation] Benefit of caffeine-potentiated chemotherapy for high-grade soft tissue sarcoma (2011)
  • Author(s): Takeuchi A, Tsuchiya H, et al
  • Organizer: International Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2011-09-17
• [Presentation] Long-term outcomes of osteosarcoma patients with a complete response to chemotherapy and who subsequently underwent intentional marginal resection (2011)
  • Author(s): Yamamoto N, Tsuchiya H, et al
  • Organizer: International Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2011-09-16
• [Presentation] Clinical outcomes following resection of well-differentiated liposarcoma (2011)
  • Author(s): Yamamoto N, Tsuchiya H, et al
  • Organizer: International Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2011-09-16
• [Presentation] Outcome of caffeine potentiated chemotherapy for patients with non-metastatic extremity osteosarcoma (2011)
  • Author(s): Shirai T, Tsuchiya H, et al
  • Organizer: International Society of Limb Salvage/Musculoskeletal Tumor Society Combined Meeting
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2011-09-16
• [Presentation] 腫瘍外科医の挑戦 (2011)
  • Author(s): 土屋弘行
  • Organizer: 第20回近整会夏季研究会(招待講演)
  • Place of Presentation: ホテルシーガルてんぽーざん大阪(大阪府)
  • Year and Date: 2011-08-27
• [Presentation] 腫瘍外科医の挑戦 (2011)
  • Author(s): 土屋弘行
  • Organizer: 第20回近整会夏季研究会
  • Place of Presentation: ホテルシーガルてんぽーざん大阪(大阪府)(招待講演)
  • Year and Date: 2011-08-27
• [Presentation] 蛍光蛋白を用いたがん細胞のin vivo 動態イメージング (2011)
  • Author(s): 山本憲男, 土屋弘行, 他
  • Organizer: 第44回日本整形外科学会 骨・軟部腫瘍学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-07-15
• [Presentation] 蛍光蛋白発現がん細胞を用いた肺転移形成のリアルタイムイメージング (2011)
  • Author(s): 木村浩明, 土屋弘行, 他
  • Organizer: 第44回日本整形外科学会 骨・軟部腫瘍学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-07-15
• [Presentation] 蛍光蛋白発現がん細胞を用いた肺転移形成のリアルタイムイメージング (2011)
  • Author(s): 木村浩明、土屋弘行, 他
  • Organizer: 第44回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-07-15
• [Presentation] 蛍光蛋白を用いたがん細胞のin vivo動態イメージング (2011)
  • Author(s): 山本憲男、土屋弘行, 他
  • Organizer: 第44回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-07-15
• [Presentation] A novel combined radiological method for evaluation of the response to chemotherapy for primary bone sarcoma (2011)
  • Author(s): Miwa S, Tsuchiya H, et al
  • Organizer: American Society of Clinical Oncology Annual Meeting
  • Place of Presentation: Mc Cormick place (USA)
  • Year and Date: 2011-06-05
• [Presentation] 骨腫瘍における再建術の進歩 (2011)
  • Author(s): 土屋弘行
  • Organizer: 第34回整形外科カレントコンセプト
  • Place of Presentation: フォーシーズンズホテル(東京都)(招待講演)
  • Year and Date: 2011-04-23
• [Presentation] Anti-β1 integrin antibody inhibits osteosarcoma lung seeding and metastasis (2011)
  • Author(s): Kimura H, Tsuchiya H, et al
  • Organizer: 102th Annual meeting of American Association for Cancer Research
  • Place of Presentation: Orange county convention center (USA)
  • Year and Date: 2011-04-04
• [Presentation] Anti-β1 integrin antibody inhibits osteosarcoma lung seeding and metastasis (2011)
  • Author(s): Kimura H, Tsuchiya H, et al
  • Organizer: 102^<th> Annual meeting of American Association for Cancer Research
  • Place of Presentation: Orange county convention center (USA)
  • Year and Date: 2011-04-04
• [Presentation] 明細胞肉腫の治療成績 (2011)
  • Author(s): 林克洋、土屋弘行, 他
  • Organizer: 第84回日本整形外科学会学術総会
  • Place of Presentation: Web開催(神奈川県)
• [Presentation] 抗がん剤・カフェイン含有リン酸カルシウムセメントによる抗腫瘍効果の増強 (2011)
  • Author(s): 丹沢義一、土屋弘行, 他
  • Organizer: 第84回日本整形外科学会学術総会
  • Place of Presentation: Web開催(神奈川県)
• [Presentation] 骨軟部腫瘍の最先端
  • Author(s): 土屋 弘行
  • Organizer: 第7回 斯整会骨軟部腫瘍検討会特別講演会
  • Place of Presentation: 東急ホテル（名古屋）
  • Invited
• [Presentation] 腫瘍再建術の最前線
  • Author(s): 土屋 弘行
  • Organizer: 兵庫医大整形外科開講記念学術講演会
  • Place of Presentation: ノボテル甲子園（兵庫）
  • Invited
• [Presentation] Biological reconstruction: Kanazawa experience
  • Author(s): Tsuchiya H
  • Organizer: Pre-congress Pediatric Orthopedic Course (Deformity Correction)
  • Place of Presentation: Hotel Iberostar (Brazil)
  • Invited
• [Presentation] 骨軟部腫瘍学の現状
  • Author(s): 土屋 弘行
  • Organizer: 第46回 東海地区整形外科教育研修会
  • Place of Presentation: ミッドランドホール（名古屋）
  • Invited
• [Remarks]
  • URL: http://ortho.w3.kanazawa-u.ac.jp/kenkyu/pages/bone_group.html