| Project/Area Number |
23390360
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Norio 金沢大学, 医薬保健学総合研究科, 特任教授 (90332668)
SHIRAI Toshiharu 京都府立医科大学, 大学院・医学研究科, 講師 (20397186)
HAYASHI Katsuhiro 金沢大学, 大学病院, 助教 (80507054)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 骨・軟部腫瘍学 / 癌 / 蛍光イメージング |
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| Research Abstract |
Fluorescent labeled osteosarcoma cell line was transplanted in nude mice and harvested in lung metastasis to obtain higher metastatic cell line. The more metastatic potential, the more PAI -1 and u-PA levels increased. We previously reported that caffeine-assisted chemotherapy. We found caffeine affects tumor cells through various pathways, such as PTEN, AKT, NF-kB, and ERK activities. Modulation of cisplatinum efficacy induced by caffeine was visualized at the subcellular level by fluorescent ubiquitination-based cell cycle indicator in the nucleus. Caffeine overcame the cell-cycle arrest induced by cisplatinum, thereby increasing its efficacy. Theobromine, a caffeine derivative, was investigated as a new anti-cancer effect. It elevates intracellular cAMP levels and inhibits cell proliferation. Surgical navigation for bone and soft tissue tumor in mouse and human was also performed using ICG and near-infrared fluorescent imaging.
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