| Project/Area Number |
23390366
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NINOMIYA Yoshifumi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70126241)
NARUSE Keiji 岡山大学, 大学院・医歯薬学総合研究科, 教授 (40252233)
NISHIDA Keiichiro 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80284058)
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| Co-Investigator(Renkei-kenkyūsha) |
OHTSUKI Takashi 岡山大学, 医学部, 客員研究員 (10534802)
OGAWA Hiroko 岡山大学, 大学病院, 助教 (70423283)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Keywords | 関節病学 / アグリカナーゼ / 関節炎 |
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| Research Abstract |
The purpose of this study was to examine the regulatory mechanism of transcriptional factors and microRNAs in chondrocyte-like cells. The analysis is important because they regulate aggrecanases that play crucial roles for osteoarthritis.
In this study, we stimulated chondrosarcoma cell lines by cytokines and mechanical stress. Then the expression level of transcriptional factors and microRNAs were investigated. In the transcriptional factors, HIF-2 was not strongly induced. We further examined microRNA alteration by microRNA array. Interestingly, the microRNA expression levels were changed by cytokine stimulation as well as mechanical stress stimulation. the expressione levels of microRNAs were devided into five groups. In conclusion, the transcriptional factors and microRNAs were regulated by cytokine stimulation and mechanical stress in the distinct pathways.
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