Regulatory mechanism of transcriptional factors and microRNA in arthritis

• Project/Area Number: 23390366
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: HIROHATA Satoshi 岡山大学, 学内共同利用施設等, 准教授 (90332791)
• Co-Investigator(Kenkyū-buntansha): NINOMIYA Yoshifumi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70126241) ; NARUSE Keiji 岡山大学, 大学院・医歯薬学総合研究科, 教授 (40252233) ; NISHIDA Keiichiro 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80284058)
• Co-Investigator(Renkei-kenkyūsha): OHTSUKI Takashi 岡山大学, 医学部, 客員研究員 (10534802) ; OGAWA Hiroko 岡山大学, 大学病院, 助教 (70423283)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: 関節病学 / アグリカナーゼ / 関節炎

Research Abstract

The purpose of this study was to examine the regulatory mechanism of transcriptional factors and microRNAs in chondrocyte-like cells. The analysis is important because they regulate aggrecanases that play crucial roles for osteoarthritis.
In this study, we stimulated chondrosarcoma cell lines by cytokines and mechanical stress. Then the expression level of transcriptional factors and microRNAs were investigated. In the transcriptional factors, HIF-2 was not strongly induced. We further examined microRNA alteration by microRNA array. Interestingly, the microRNA expression levels were changed by cytokine stimulation as well as mechanical stress stimulation. the expressione levels of microRNAs were devided into five groups. In conclusion, the transcriptional factors and microRNAs were regulated by cytokine stimulation and mechanical stress in the distinct pathways.

Research Products

• [Journal Article] ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor (2014)
  • Author(s): Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek MZ, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
  • Journal Title: Exp Cell Res Volume: 323(2) Issue: 2 Pages: 263-75
  • DOI: 10.1016/j.yexcr.2014.03.002
  • Peer Reviewed / Open Access
• [Journal Article] Vascular endothelial growth factor C-induced lymphangiogenesis decreases tumor interstitial fluid pressure and tumor (2013)
  • Author(s): Hofmann M, Pflanzer R, Zoller NN, Bernd A, Kaufmann R, Thaci D, Bereiter-Hahn J, Hirohata S, Kippenberger S
  • Journal Title: Transl Oncol Volume: 6(4) Pages: 398-404
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730014/
  • Peer Reviewed
• [Journal Article] ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse (2013)
  • Author(s): Demircan K, Yonezawa T, Takigawa T, Topcu V, Erdogan S, Ucar F, Armutcu F, Yigitoglu MR, Ninomiya Y, Hirohata S
  • Journal Title: Neurosci Lett Volume: 544 Pages: 25-30
  • DOI: 10.1016/j.neulet.2013.02.064
  • Peer Reviewed
• [Journal Article] Eosinophil cationic protein enhances stabilization of β-catenin during cardiomyocyte differentiation in P19CL6 embryonal carcinoma cells (2013)
  • Author(s): Jin G, Mizutani A, Fukuda T, Otani T, Yan T, Prieto Vila M, Murakami H, Kudoh T, Hirohata S, Kasai T, Salomon DS, Seno M
  • Journal Title: Mol Biol Rep Volume: 40(4) Issue: 4 Pages: 3165-3171
  • DOI: 10.1007/s11033-012-2390-5
  • Peer Reviewed
• [Journal Article] Augmentation of ADAMTS9 gene expression by IL-1β is reversed by NFκB and MAPK inhibitors, but not PI3 kinase inhibitors (2013)
  • Author(s): Uysal S, Unal ZN, Erdoğan S, Akyol S, Ramazan Yiğitoğlu M, Hirohata S, Işik B, Demircan K
  • Journal Title: Cell Biochem Funct Volume: 33(6) Issue: 7 Pages: 409-18
  • DOI: 10.1002/cbf.2932
  • Peer Reviewed
• [Journal Article] Vascular endothelial growth factor C-induced lymphangiogenesis decreases tumor interstitial fluid pressure and tumor. (2013)
  • Author(s): Hofmann M, Pflanzer R, Zoller NN, Bernd A, Kaufmann R, Thaci D, Bereiter-Hahn J, Hirohata S, Kippenberger S.
  • Journal Title: Transl Oncol. Volume: 6(4) Pages: 698-404
  • Peer Reviewed
• [Journal Article] Eosinophil cationic protein enhances cardiomyocyte differentiation of P19CL6 embryonal carcinoma cells by stimulating the FGF receptor signaling pathway (2012)
  • Author(s): Jin G, Mizutani A, Fukuda T, Chen L, Nakanishi K, Yan T, Kudoh T, Hirohata S, Kasai T, Murakami H, Salomon DS, Seno M
  • Journal Title: Growth Factors Volume: 30(5) Issue: 5 Pages: 344-355
  • DOI: 10.3109/08977194.2012.709852
  • Peer Reviewed
• [Journal Article] Tumor growth inhibitory effect of ADAMTS1 is accompanied by the inhibition of tumor angiogenesis. (2012)
  • Author(s): Obika M
  • Journal Title: Cancer Science Volume: 10 Issue: 10 Pages: 1889-1897
  • DOI: 10.1111/j.1349-7006.2012.02381.x
  • Peer Reviewed
• [Journal Article] Elevated serum adipocyte fatty acid-binding protein concentrations are independently associated with renal dysfunction in patients with stable angina pectoris (2012)
  • Author(s): Iwamoto M, Miyoshi T, Doi M, Takeda K, Kajiya M, Nosaka K, Nakamaya R, Hirohata S, Usui S, Kusachi S, Sakane K, Nakamura K, Ito H
  • Journal Title: Cardiovasc Diabetol Volume: 11(1) Issue: 1 Pages: 26-26
  • DOI: 10.1186/1475-2840-11-26
  • Peer Reviewed
• [Journal Article] Four-year clinical outcomes of the OLIVUS-Ex (impact of Olmesartan on progression of coronary atherosclerosis : Evaluation by intravascular ultrasound) extension trial (2012)
  • Author(s): Hirohata A, Yamamoto K, Miyoshi T, Hatanaka K, Hirohata S, Yamawaki H, Komatsubara I, Hirose E, Kobayashi Y, Ohkawa K, Ohara M, Takafuji H, Sano F, Toyama Y, Kusachi S, Ohe T, Ito H
  • Journal Title: Atherosclerosis Volume: 220(1) Issue: 1 Pages: 134-138
  • DOI: 10.1016/j.atherosclerosis.2011.10.013
  • Peer Reviewed
• [Journal Article] Sufficient pulmonary vein image quality of non-enhanced multi-detector row computed tomography for pulmonary vein isolation by catheter ablation (2012)
  • Author(s): Yamaji H, Hina K, Kawamura H, Murakami T, Murakami M, Hirohata S, Ohmaru N, Kusachi S
  • Journal Title: Europace 2012 Volume: 14(1) Issue: 1 Pages: 52-59
  • DOI: 10.1093/europace/eur284
  • Peer Reviewed
• [Journal Article] Sufficient pulmonary vein image quality of non-enhanced multi-detector row computed tomography for pulmonary vein isolation by catheter ablation (2012)
  • Author(s): Yamaji H et al.,
  • Journal Title: Europace Volume: 14(1) Pages: 52-59
  • Peer Reviewed
• [Journal Article] Four-year clinical outcomes of the OLIVUS-Ex (impact of Olmesartan on progression of coronary atherosclerosis: Evaluation by intravascular ultrasound) extension trial (2012)
  • Author(s): Hirohata A et al.,
  • Journal Title: Atherosclerosis Volume: 220 (1) Pages: 134-138
  • Peer Reviewed
• [Journal Article] Elevated serum adipocyte fatty acid-binding protein concentrations are independently associated with renal dysfunction in patients with stable angina pectoris. (2012)
  • Author(s): Iwamoto M et al.,
  • Journal Title: Cardiovasc Diabetol Volume: 11 (1) Pages: 26-26
  • Peer Reviewed
• [Journal Article] The tumor growth inhibitory effect of ADAMTS1 is accompanied by the inhibition of tumor angiogenesis (2012)
  • Author(s): Obika M et al.,
  • Journal Title: Cancer Sci. Volume: 103 (10) Pages: 1889-1897
  • Peer Reviewed
• [Journal Article] Eosinophil cationic protein enhances cardiomyocyte differentiation of P19CL6 embryonal carcinoma cells by stimulating the FGF receptor signaling pathway (2012)
  • Author(s): Jin G et al.,
  • Journal Title: Growth Factors Volume: 30 (5) Pages: 344-355
  • Peer Reviewed
• [Journal Article] Serum hepatitis B virus DNA before liver transplantation correlates with HBV reinfection rate even under successful low-dose hepatitis B immunoglobulin prophylaxis (2011)
  • Author(s): Yasunaka T, Takaki A, Yagi T, Iwasaki Y, Sadamori H, Koike K, Hirohata S, Tatsukawa M, Kawai D, Shiraha H, Miyake Y, Ikeda F, Kobashi H, Matsuda H, Shinoura S, Yoshida R, Satoh D, Utsumi M, Onishi T, Yamamoto K
  • Journal Title: Hepatol Int Volume: 5(4) Pages: 918-926
  • URL: http://link.springer.com/article/10.1007%2Fs12072-011-9265-z
  • Peer Reviewed
• [Journal Article] Distribution pattern of urine albumin creatinine ratio and the prevalence of high-normal levels in untreated asymptomatic non-diabetic hypertensive patients (2011)
  • Author(s): Ohmaru N, Nakatsu T, Izumi R, Mashima K, Toki M, Kobayashi A, Ogawa H, Hirohata S, Ikeda S, Kusachi S
  • Journal Title: Intern Med Volume: 50(16) Pages: 1621-9
  • NAID: 130000969361
  • URL: https://www.jstage.jst.go.jp/article/internalmedicine/50/16/50_16_1621/_article
  • Peer Reviewed
• [Journal Article] Association of increased plasma adipocyte fatty acid-binding protein with coronary artery disease in non-elderly men (2011)
  • Author(s): DoiM, MiyoshiT, HirohataS, NakamuraK, UsuiS, TakedaK, IwamotoM, KusachiS, KusanoK, ItoH
  • Journal Title: Cardiovascular Diabetology 2011 Volume: 10 Issue: 1 Pages: 44-44
  • DOI: 10.1186/1475-2840-10-44
  • Peer Reviewed
• [Journal Article] Olmesartan reduces arterial stiffness and serum adipocyte fatty acid-binding protein in hypertensive patients (2011)
  • Author(s): Miyoshi T, ら
  • Journal Title: Heart Vessels Volume: 26 Issue: 4 Pages: 408-413
  • DOI: 10.1007/s00380-010-0060-x
  • Peer Reviewed
• [Journal Article] Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells (2011)
  • Author(s): Tetsunaga T, et al.
  • Journal Title: Osteoarthritis Cartilage Volume: 19(2) Issue: 2 Pages: 222-232
  • DOI: 10.1016/j.joca.2010.11.004
  • Peer Reviewed
• [Journal Article] Association of serum levels of arachidonic acid and eicosapentaenoic acid with prevalence of major adverse cardiac events after acute myocardial infarction (2011)
  • Author(s): Ueeda M, Doumei T, Takaya Y, Ohnishi N, Takaishi A, Hirohata S, Miyoshi T, Shinohata R, Usui S, Kusachi S
  • Journal Title: Heart Vessels Volume: 26(2) Issue: 2 Pages: 145-52
  • DOI: 10.1007/s00380-010-0038-8
  • Peer Reviewed
• [Journal Article] AHR, a novel acute hypoxia-response sequence, drives reporter gene under hypoxia in vitro and in vivo (2011)
  • Author(s): Cilek MZ, et al.
  • Journal Title: Cell Biol Int Volume: 35 Issue: 1 Pages: 1-8
  • DOI: 10.1042/cbi20100290
  • Peer Reviewed
• [Presentation] ADAMTS1 was differently regulated in breast cancer cells (2014)
  • Author(s): Asano K, Hirohata S, Cilek MZ, Hatipoglu OF, Ohtsuki T, Inagaki J, Ninomiya Y
  • Organizer: 第7回高度医療都市を創出する未来技術国際シンポジウム
  • Place of Presentation: 岡山市
  • Year and Date: 2014-02-07
• [Presentation] ヒアルロン酸の炎症性サイトカイン誘導性ヒアルロン酸合成酵素分子種への影響 (2014)
  • Author(s): 大月孝志, 廣畑 聡, 平田 彩, 浅野恵一, 楠絵里子, 稲垣純子, 西田圭一郎, 二宮善文
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都市
• [Presentation] リジンオリゴマーからなる関節軟骨特異的X 線プローブの創出 (2014)
  • Author(s): 大橋俊孝, 芳谷 学, 大野充昭, 山田翔也, 大月孝志, 前原亜美, 廣畑 聡, 西田圭一郎, 窪木拓男, 加来田博貴, 二宮善文
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都市
• [Presentation] Red cell distribution width is an important factor for evaluatiin of diastolic dysfunction in chronic hemodialysis patients (2014)
  • Author(s): Kumashiro H, Hirohata S, Yamanishi A, Tomioka C, Kusachi S
  • Organizer: 第78回日本循環器学会総会
  • Place of Presentation: 東京都
• [Presentation] リジンオリゴマーからなる関節軟骨特異的X線プローブの創出 (2014)
  • Author(s): 大橋俊孝、芳谷 学、大野充昭、山田翔也、大月孝志、前原亜美、廣畑 聡、西田圭一郎、窪木拓男、加来田博貴、二宮善文
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都市
• [Presentation] ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor (2014)
  • Author(s): Junko Inagaki, Katsuyuki Takahashi, Hiroko Ogawa, Omer Hatipoglu, Mehmet Cilek, Masanari Obika, Takashi Ohtsuki, Shozo Kusachi, Yoshifumi Ninomiya, Satoshi Hirohata
  • Organizer: The 18th International Vascular Biology Meeting
  • Place of Presentation: 京都
• [Presentation] Novel vector construct driven under acute hypoxia (2013)
  • Author(s): Asano K, Hirohata S, Cilek MZ, Hatipoglu OF, Ohtsuki T, Inagaki J, Ninomiya Y
  • Organizer: 第6回高度医療都市を創出する未来技術国際シンポジウム
  • Place of Presentation: 岡山市
• [Presentation] ADAMTS1 null mice demonstrated omphalocele phenotype (2013)
  • Author(s): Hirohata S, Hatipoglu OF, Kusunoki E, Ohtsuki T, Inagaki J, Kusachi S, Ninomiya Y
  • Organizer: matrix metalloproteinase ポスター発表ゴードンカンファレンス
  • Place of Presentation: イタリア・バルガ市
• [Presentation] Red cell distribution width is an effective tool for detecting diastolic dysfunction in chronic hemodialysis patients (2013)
  • Author(s): Kumashiro H, Hirohata S, Yamanishi A, Tomioka C, Kusachi S
  • Organizer: 欧州心不全会議(ESC)
  • Place of Presentation: ポルトガル リスボン ポスター発表
• [Presentation] Cyclic tensile strain(CTS)による炎症性サイトカイン誘導性細胞応答に対する抑制機構 micro RNA array による解析 (2013)
  • Author(s): 大月孝志, 川地輝幸, 平田彩, 浅野恵一, 楠絵理子, 稲垣純子, 熊岸加苗, 西田圭一郎, 二宮善文, 廣畑 聡
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸市
• [Presentation] Super-elderly Patients of Acute Heart Failure Need Special Program of Cardiac Rehabilitation (2013)
  • Author(s): Masuda T, Takaishi A, Amioka N, Arai Y, Yamada D, Ohnishi N, Ueeda M, Itou H, Hirohata S, Kusachi S
  • Organizer: 第77回日本循環器学会総会
  • Place of Presentation: 横浜市
• [Presentation] MDA-LDL and Arachidonic Acids are Higher in Patients with Younger ACS than Elder, but Only AA in Younger SAP (2013)
  • Author(s): Arai Y, Ueeda M, Takaishi A, Ohnishi N, Yamada D, Masuda T, Amioka N, Hirohata S, Kusachi S, Itou H
  • Organizer: 第77回日本循環器学会総会
  • Place of Presentation: 横浜市
• [Presentation] Aging and CKD were the Main Risk Factors for AMI with Low LDL-C Level (2013)
  • Author(s): Arai Y, Ueeda M, Takaishi A, Ohnishi N, Yamada D, Masuda T, Amioka N, Hirohata S, Kusachi S, Itou H
  • Organizer: 第77回日本循環器学会総会
  • Place of Presentation: 横浜市
• [Presentation] Serum Adipocyte Fatty Acid-binding Protein is a Predictor of Cardiovascular Events in Patients with Stable Angina Undergoing Percutaneous Coronary Intervention (2013)
  • Author(s): Takagi W, Kajiya M, Nosaka K, Ohkawa K, Nakayama R, Doi M, Iwamoto M, Takeda K, Hirohata S, Kusachi S, Toru Miyoshi T, Hiroshi Itoh H
  • Organizer: 第77回日本循環器学会総会
  • Place of Presentation: 横浜市
• [Presentation] Association of Secreted Frizzled-related Protein 5, a Novel Anti-inflammatory Adipokine, with Coronary Artery Disease in Non-elderly Population (2013)
  • Author(s): Nosaka K, Miyoshi T, Doi M, Iwamoto M, Kajiya M, Ohkawa K, Nakayama R, Takagi W, Ko Takeda K, Hirohata S, Kusachi S, Itoh H
  • Organizer: 第77回日本循環器学会総会
  • Place of Presentation: 横浜市
• [Presentation] ヒアルロン酸(HA)分子量と関節軟骨保護効果の解析(in vitro & in vivo) (2013)
  • Author(s): 大月孝志, 廣畑 聡, 浅野恵一, 楠絵里子, 稲垣純子, 西田圭一郎, 二宮善文
  • Organizer: 第45回日本結合組織学会学術大会 第60回マトリックス研究会大会
  • Place of Presentation: 和歌山市
• [Presentation] Cyclic tensile strain (CTS) による炎症性サイトカイン誘導性ADAMTS ファミリー発現抑制機構へのメカノセンサーの関与 (2013)
  • Author(s): 大月孝志, 廣畑 聡, 西田圭一郎, 二宮善文
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] ADAMTS1 null mice demonstrated omphalocele phenotype (2013)
  • Author(s): Satoshi Hirohata, Omer Faruk Hatipoglu, Eriko Kusunoki, Takashi Ohtsuki, Junko Inagaki, Shozo Kusachi, Yoshifumi Ninomiya
  • Organizer: ゴードンカンファレンス matrix metalloproteinase
  • Place of Presentation: イタリア・バルガ市
• [Presentation] Red cell distribution width is an effective tool for detecting diastolic dysfunction in chronic hemodialysis patients (2013)
  • Author(s): Hirofumi Kumashiro, Satoshi Hirohata, Asami Yamanishi, Chiho Tomioka, Shozo Kusachi
  • Organizer: 欧州心不全会議(ESC)
  • Place of Presentation: ポルトガル・リスボン市
• [Presentation] ヒアルロン酸(HA)分子量と関節軟骨保護効果の解析（in vitro & in vivo） (2013)
  • Author(s): 大月孝志 廣畑 聡 浅野恵一 楠絵里子 稲垣純子 西田圭一郎 二宮善文
  • Organizer: 第４５回日本結合組織学会学術大会 第６０回マトリックス研究会大会
  • Place of Presentation: 和歌山県和歌山市
• [Presentation] Novel vector construct driven under acute hypoxia (2013)
  • Author(s): Keiichi Asano
  • Organizer: 第６回高度医療都市を創出する未来技術国際シンポジウム
  • Place of Presentation: 岡山市
• [Presentation] Cyclic tensile strain (CTS) による炎症性サイトカイン誘導性ADAMTSファミリー発現抑制機構へのメカノセンサーの関与 (2013)
  • Author(s): 大月孝志
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 大阪市
• [Presentation] Altered Expression of Hypoxia Response in Ischemic Hind Limb in ADAMTS1 Hetero Mice (2012)
  • Author(s): ZC Mehmet, Hirohata S, Ogawa H, Miyoshi T, Kusachi S, Ninomiya Y
  • Organizer: 第5回高度医療都市を創出する未来技術国際シンポジウム
  • Place of Presentation: 岡山市
  • Year and Date: 2012-03-15
• [Presentation] Altered Expression of Hypoxia Response in Ischemic Hind Limb m ADAMTS1 Hetero Mice (2012)
  • Author(s): Cilek, et al.
  • Organizer: 第5回高度医療都市を創出する未来技術国際シンポジウム
  • Place of Presentation: 岡山大学50周年記念館(岡山)
  • Year and Date: 2012-03-15
• [Presentation] The tumor growth inhibitory effect of ADAMTS1 is accompanied by the inhibition of tumor angiogenesis (2012)
  • Author(s): Hirohata S, Ohtsuki T, Obika M, Ogawa H, Inagaki J, Kusachi S, Ninomiya Y
  • Organizer: 2012 Biennial Meeting of American Society for Matrix Biology
  • Place of Presentation: 米国 San Diego
• [Presentation] Cyclic tensile strain inhibits Interleukin-1β and Tumor Necrosis Factor-α induced aggrecanase in human chondrosarcoma cell line OUMS-27 by stretch-activated channels (2012)
  • Author(s): Ohtsuki T, Nishida K, Hirohata S, Ninomiya Y
  • Organizer: 2012 Biennial Meeting of American Society for Matrix Biology
  • Place of Presentation: 米国 San Diego
• [Presentation] The inhibitory effect of an angiotensin II converting enzyme inhibitor on gelatinase activity in experimental abdominal aortic aneurysm (2012)
  • Author(s): Miyoshi T, Yonezawa T, Nakamura K, Hirohata S, Ninomiya Y, Ito H
  • Organizer: 第20回日本血管生物医学会
  • Place of Presentation: 徳島市
• [Presentation] ンパ管内皮細胞に対するADAMTS1 の作用とシグナル伝達 (2012)
  • Author(s): 稲垣純子, 高橋克之, 小川弘子, Hatipoglu OF, ZC Mehmet, 小比賀真就, 廣畑 聡, 二宮善文
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡市
• [Presentation] ADAMTS1 inhibits angiogenesis by inducing apoptosis specifically on endothelial cell (2012)
  • Author(s): 小比賀真就, 廣畑 聡, 幡中邦彦, 小川弘子, 三好 亨, 伊藤 浩, 草地省蔵, 二宮善文
  • Organizer: 第76回日本循環器学会総会
  • Place of Presentation: 福岡市
• [Presentation] ADAMTS1 plays a role in apoptosis by mediating TNF-α receptor1 (2012)
  • Author(s): 小比賀真就, 廣畑 聡, 幡中邦彦, 小川弘子, 三好 亨, 伊藤 浩, 草地省蔵, 二宮善文
  • Organizer: 第76回日本循環器学会総会
  • Place of Presentation: 福岡市
• [Presentation] Impact of fluorescent imaging with E-selectin-targeted liposome on non-invasie assessment of therapeutic effect for atherosclerosis in mice (2012)
  • Author(s): 幡中邦彦, 小川弘子, 小比賀真就, 伊藤浩, 廣畑 聡
  • Organizer: 第76回日本循環器学会総会
  • Place of Presentation: 福岡市
• [Presentation] Impact of collagen gene deficiency on the intimal hyperplasia after angioplasty (2012)
  • Author(s): 小川弘子, 廣畑 聡, 小比賀真就, 幡中邦彦, 伊藤 浩, 佐田正隆, 草地省蔵
  • Organizer: 第76回日本循環器学会総会
  • Place of Presentation: 福岡市
• [Presentation] メカニカルストレスとサイトカイン (2012)
  • Author(s): 大月孝志, 廣畑 聡, 西田圭一郎, 二宮善文
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: 名古屋市
• [Presentation] ADAMTS1ノックアウトマウスの解析 (2012)
  • Author(s): 廣畑 聡, ハティポール・オメル・ファルク, 楠絵理子, メフメット・ゼイネル・チレッキ, 大月孝志, 稲垣純子, 草地省蔵, 二宮善文
  • Organizer: 第44回日本結合組織学会学術大会 第59回マトリックス研究会大会
  • Place of Presentation: 東京
• [Presentation] ヒアルロン酸(HA)分子量と関節軟骨保護効果の解析 (2012)
  • Author(s): 大月孝志, メフメット・ゼイネル・チレッキ, ハティポール・オメル・ファルク, 西田圭一郎, 二宮善文, 廣畑 聡
  • Organizer: 第44回日本結合組織学会学術大会 第59回マトリックス研究会大会
  • Place of Presentation: 東京
• [Presentation] The tumor growth inhibitory effect of ADAMTS1 is accompanied by the inhibition of tumor angiogenesis (2012)
  • Author(s): Satoshi Hirohata
  • Organizer: 2012 Biennial Meeting of American Society for Matrix Biology
  • Place of Presentation: 米国 サンディエゴ
• [Presentation] Cyclic tensile strain inhibits Interleukin-1β and Tumor Necrosis Factor-α induced aggrecanase in human chondrosarcoma cell line OUMS-27 by stretch-activated channels (2012)
  • Author(s): Takashi Ohtsuki
  • Organizer: 2012 Biennial Meeting of American Society for Matrix Biology
  • Place of Presentation: 米国 サンディエゴ
• [Presentation] リンパ管内皮細胞に対するADAMTS1の作用とシグナル伝達 (2012)
  • Author(s): 稲垣 純子
  • Organizer: 第８５回日本生化学会大会
  • Place of Presentation: 福岡市
• [Presentation] ADAMTS1ノックアウトマウスの解析 (2012)
  • Author(s): 廣畑 聡
  • Organizer: 第４４回日本結合組織学会学術大会 第５９回マトリックス研究会大会
  • Place of Presentation: 東京都
• [Presentation] ヒアルロン酸(HA)分子量と関節軟骨保護効果の解析 (2012)
  • Author(s): 大月孝志
  • Organizer: 第４４回日本結合組織学会学術大会 第５９回マトリックス研究会大会
  • Place of Presentation: 東京都
• [Presentation] Attenuation of endothelial apoptosis induced by TNF-alpha in ADAMTS1 deficient cells (2011)
  • Author(s): Hirohata S, Hatipoglu OF, ZC Mehmet, Obika M, Ogawa H, Kusachi S, Ninomiya Y
  • Organizer: ゴードンカンファレンス matrix metalloproteinase
  • Place of Presentation: 米国・ロードアイランド州
• [Presentation] ADAMTS1 is a novel acute biphasic marker for ischemia and reperfusion in myocardial infarction (2011)
  • Author(s): Hirohata S, Ogawa H, Miyoshi T, Obika M, Ninomiya Y, Kusachi S, Usui S, Shinohata R, Ito H
  • Organizer: 欧州心臓学会議(ESC)
  • Place of Presentation: フランス パリ
• [Presentation] ADAMTS1 plays roles in endothelial cell apoptosis (2011)
  • Author(s): Obika M, Hirohata S, Hatipoglu OF, ZC Mehmet, Ogawa H, Hatanaka K, Miyoshi T, Kusachi S, Ninomiya Y
  • Organizer: アメリカ心臓協会(AHA) 学術集会
  • Place of Presentation: 米国・オーランド
• [Presentation] Hyaluronan inhibits aggrecanase in human chondrosarcoma cell line OUMS-27 in a size dependent manner (2011)
  • Author(s): Ohtsuki T, Nishida K, Hirohata S, Ninomiya Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 急性冠症候群患者における血清ADAMTS1レベルの上昇 (2011)
  • Author(s): 廣畑 聡, 小比賀真就, 幡中邦彦, 小川弘子, 三好 亨, 石井裕子, 坂本かおり, 草地省蔵, 伊藤 浩, 二宮善文
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山市
• [Presentation] 草地省蔵 非糖尿病性未治療高血圧患者におけるUACR の分布とhigh-normal の頻度 (2011)
  • Author(s): 小川弘子, 大丸奈月, 中津高明, 泉 礼司, 間島圭一, 土岐美沙子, 小林亜紗子, 廣畑 聡, 池田 敏
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山市
• [Presentation] ARB 内服中高血圧患者に対するカルシウム拮抗薬もしくは利尿剤の追加がAugmentation index へ与える影響 (2011)
  • Author(s): 三好 亨, 土井正行, 小川弘子, 廣畑 聡, 草地省蔵, 小出典男, 伊藤 浩
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山市
• [Presentation] 発作性心房細動の出現率に対するarterial stiffness 増加の影響 (2011)
  • Author(s): 小川弘子, 三好亨, 土井正行, 廣畑 聡, 草地省蔵, 小出典男
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山市
• [Presentation] 虚血性心疾患患者によるCAVI と冠動脈硬化, 左心機能の関連性の検討 (2011)
  • Author(s): 小川弘子, 三好 亨, 土井正行, 廣畑 聡, 草地省蔵, 小出典男
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山市
• [Presentation] ADAMTS1のin vitro でのリンパ管新生阻害効果 (2011)
  • Author(s): 稲垣純子, 高橋克之, 小川弘子, Omer F. Hatipoglu, M. Zeynel Cilek, 小比賀真就, 米澤朋子, 大橋俊孝, 廣畑 聡, 二宮善文
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
• [Presentation] ADAMTS1は血管新生を阻害しアポトーシスを誘導する (2011)
  • Author(s): 廣畑 聡, 小比賀真就, オメル・ファルク・ハティポール, 小川弘子, メフメット・ゼェイネル・チレッキ, 稲垣純子, 大月孝志 石井裕子, 幡中邦彦, 草地省蔵, 米澤朋子, 大橋俊孝, 二宮善文
  • Organizer: 第43回日本結合組織学会学術大会・第58回マトリックス研究会大会 合同学術集会
  • Place of Presentation: 別府市
• [Presentation] ADAMTS1 は腫瘍壊死因子刺激下の内皮細胞におけるアポトーシスに関連する (2011)
  • Author(s): オメル・ファルク・ハティポール, 小比賀真就, 廣畑 聡, 小川弘子, メフメット・ゼェイネル・チレッキ, 稲垣純子, 大月孝志, 石井裕子, 幡中邦彦, 草地省蔵, 米澤朋子, 大橋俊孝, 二宮善文
  • Organizer: 第43回日本結合組織学会学術大会・第58回マトリックス研究会大会 合同学術集会
  • Place of Presentation: 別府市
• [Presentation] アンジオテンシンII受容体拮抗薬(オルメサルタン)によるサイトカイン発現抑制効果と心機能保持効果 (2011)
  • Author(s): 大月孝志, 篠畑綾子, 廣畑 聡, 草地省蔵, 二宮善文
  • Organizer: 第43回日本結合組織学会学術大会・第58回マトリックス研究会大会合同学術集会
  • Place of Presentation: 別府市
• [Presentation] A ratio of MDA-LDL to LDL-C represents plaque vulnerability in a patient with angina pectoris : VH-IVUS study (2011)
  • Author(s): Otsuka H, Ueeda M, Kagawa K, Arai Y, Ugawa S, Ohnishi N, Takaishi A, Hirohata S, Ito H, Kusachi S
  • Organizer: 第43回日本動脈硬化学会学術集会
  • Place of Presentation: 札幌市
• [Presentation] Endothelial cell-specific early immediate response gene expression in hypoxia (2011)
  • Author(s): Hirohata S, Mehmet C, Obika M, Ogawa H, Hatipoglu F, Miyoshi T, Kusachi S, Ninomiya Y
  • Organizer: 第43回日本動脈硬化学会学術集会
  • Place of Presentation: 札幌市
• [Presentation] The role of alpha556 chains of type IV collagen in restenosis after angioplasty (2011)
  • Author(s): Ogawa H, Yonezawa T, Sata M, Hirohata S, Oohashi T, Kusachi S, Ninomiya Y
  • Organizer: 第43回日本動脈硬化学会学術集会
  • Place of Presentation: 札幌市
• [Presentation] ADAMTS1 plays roles in endothelial cell apoptosis (2011)
  • Author(s): Obika, et al.
  • Organizer: アメリカ心臓協会(AHA)学術集会
  • Place of Presentation: 米国オーランド市
• [Presentation] Hyaluronan inhibits aggrecanase in human chondrosarcoma cell line OUMS-27 in a size dependent manner (2011)
  • Author(s): Ohtsuki, et al.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] Endothelial cell-specific early immediate response gene expression in hypoxia (2011)
  • Author(s): Hirohata, et al.
  • Organizer: 第43回日本動脈硬化学会学術集会
  • Place of Presentation: ロイトン札幌(札幌)
• [Presentation] ADAMTS1は血管新生を阻害しアポトーシスを誘導する (2011)
  • Author(s): 廣畑, et al.
  • Organizer: 第43回日本結合組織学会学術大会・第58回マトリックス研究会大会合同学術集会
  • Place of Presentation: 別府ビーコンプラザ(大分)
• [Presentation] ADAMTS1は腫瘍壊死因子刺激下の内皮細胞におけるアポトーシスに関連する (2011)
  • Author(s): ハテイポール, et al.
  • Organizer: 第43回日本結合組織学会学術大会・第58回マトリックス研究会大会合同学術集会
  • Place of Presentation: 別府ビーコンプラザ(大分)
• [Book] 血管新生研究の最先端 佐藤靖史・高倉伸幸編12 章 2)ADAMTS の機能 (2013)
  • Author(s): 廣畑 聡, 二宮善文
  • Publisher: 医薬ジャーナル社
• [Book] 血管新生研究の最先端 (2013)
  • Author(s): 佐藤靖史・高倉伸幸 編
  • Total Pages: 331
  • Publisher: 医薬ジャーナル社
• [Remarks]
  • URL: http://mbb-okayama.sakura.ne.jp/
• [Patent(Industrial Property Rights)] 新規DNA 断片およびその用途 (2014)
  • Inventor(s): 廣畑 聡, 二宮善文, 草地省蔵, オメル・ファルク ハティポール
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-14
• [Patent(Industrial Property Rights)] ECP を有効成分とする左室リモデリングの予防及び治療剤 (2014)
  • Inventor(s): 妹尾昌治, 多田宏子, 福田隆之, 廣畑 聡, 丸山昌彦, 草地省蔵, 二宮善文, 五十嵐貢一
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-02-07
• [Patent(Industrial Property Rights)] がん細胞特異的遺伝子発現法を用いた血管新生阻害薬 (2011)
  • Inventor(s): 廣畑 聡, 三好 亨, 土井正行, 小川弘子, 二宮善文
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2011-10-21