Development of advanced fluorescent imaging systems for the study of bone remodeling

• Project/Area Number: 23390368
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Ehime University
• Principal Investigator: IMAMURA Takeshi 愛媛大学, 医学(系)研究科(研究院), 教授 (70264421)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: バイオテクノロジー / シグナル伝達 / 遺伝子 / タンパク質 / 細胞・組織

Research Abstract

To clarify the molecular mechanisms of coupling between bone formation and resorption during bone remodeling, we tried to establish intravital fluorescent imaging system by which cell behavior, cell differentiation and apoptosis of osteoblasts and osteoclasts can be examined in vivo. In the present study, we established in vivo fluorescent imaging modality and generated several transgenic mice for imaging of osteoblasts and osteoclasts. Using CatK-tdTomato-Tg mouse, we could visualize osteoclast in vitro and in vivo.

Research Products

• [Journal Article] Second Harmonic Generation Reveals Collagen Fibril Remodeling in Fibroblast-populated Collagen Gels (2013)
  • Author(s): Toki F, Honkura N, Shirakata Y, Imamura T, Higashiyama S, Nanba D
  • Journal Title: Cell Struct Funct Volume: 38 Pages: 227-236
  • NAID: 130004137598
  • Peer Reviewed
• [Journal Article] Second Harmonic Generation Reveals Collagen Fibril Remodeling in Fibroblast-populated Collagen Gels (2013)
  • Author(s): Toki F, Honkura N, Shirakata Y, Imamura T, Higashiyama S, Nanba D
  • Journal Title: Cell Structure and Function Volume: 38 Issue: 2 Pages: 229-238
  • DOI: 10.1247/csf.13017
  • NAID: 130004137598
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] HTLV-1 bZIP factor enhances TGF-β signaling through p300 coactivator (2011)
  • Author(s): Zhao T
  • Journal Title: Blood Volume: 118 Issue: 7 Pages: 1865-1876
  • DOI: 10.1182/blood-2010-12-326199
  • Peer Reviewed
• [Journal Article] Regulation of RANKL-induced osteoclastogenesis by TGF-b through molecular interaction between Smad3 and Traf6 (2011)
  • Author(s): Yasui T, Kadono Y, Nakamura M, Oshima Y, Matsumoto T, Masuda H, Hirose J, Omata Y, Yasuda H, Imumara T, Nakamura K, Tanaka S.
  • Journal Title: J Bone Miner Res Volume: 26(7) Issue: 7 Pages: 1447-1456
  • DOI: 10.1002/jbmr.357
• [Presentation] 生体蛍光イメージング技術が拓く次世代骨研究戦略 (2013)
  • Author(s): 今村健志
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山コンベンションセンター, 岡山市
  • Year and Date: 2013-09-21
• [Presentation] カレントコンセプト5骨髄環境と幹細胞研究の最前線光技術を駆使したがんと骨髄のイメージング (2011)
  • Author(s): 今村健志
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場大阪市(招待講演)
  • Year and Date: 2011-07-29
• [Presentation] ランチョンセミナー3光技術を駆使した時空間機能形態学 (2011)
  • Author(s): 今村健志
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜横浜市(招待講演)
  • Year and Date: 2011-04-28
• [Presentation] 生体蛍光イメージング技術が拓く次世代骨研究戦略
  • Author(s): 今村健志
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山コンベンションセンター、岡山市
  • Invited
• [Presentation] MTE3「イメージング」
  • Author(s): 今村健志
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル
  • Invited