Molecular mechanisms of alveolar epithelial healing process in acute lung injury
| Project/Area Number |
23390376
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
SUGAHARA Kazuhiro 琉球大学, 医学(系)研究科(研究院), 教授 (20171126)
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| Co-Investigator(Kenkyū-buntansha) |
OSHIRO Masakatsu 琉球大学, 医学部附属病院, 助教 (00315483)
TERUYA Koji 琉球大学, 大学院・医学研究科, 助教 (50437985)
MIYATA Yuji 琉球大学, 医学部附属病院, 講師 (60315471)
NOGUCHI Nobuhiro 琉球大学, 医学部附属病院, 助教 (80457671)
IZUMI Syunsuke 琉球大学, 医学部附属病院, 助教 (90649401)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 急性肺傷害 / 創傷治癒 / 肺胞上皮細胞 / 細胞増殖因子 / EGFR / サイトカイン / NF-kB / EMT / エチルピルビン酸 / epithelial-mesenchymal transition |
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| Research Abstract |
1. We have demonstrated that 1) keratinocyte growth factor (KGF) is a potent factor for proliferation and differentiation of alveolar type II cells, 2) intratracheal instillation of KGF prevented acute lung injury by bleomycin and HCl. 2. Ethyl pyruvate reportedly has anti-inflammatory actions through inhibition of NF-kB. We confirmed that treatment of cultured alveolar epithelial cells with tumor necrosis factor a (TNFa) increased the NF-kB/DNA-binding activity, which was strongly inhibited with incubating with ethyl pyruvate. We also demonstrated that ethyl pyruvate interacts with RelA and p50 to inhibit their functions at multiple points. 3. Legionella pneumophila has flagella, causes pulmonary fibrosis. Microarray analysis in cultured alveolar epithelial cells indicated that flagellin increased fibronectin level and reduced E-cadherin level, which suggested that flagellin cooperates with TGF-b and induces EMT in alveolar epithelial cells, and causes fibrosis.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Long-term treatment with San'o-shashin-to, Kampo medicine, markedly ameliorates cardiac ischemia-reperfusion injury in ovariectomized rats via the Redox-dependent mechanism2013
Author(s)
Mayuko Sakanashi, T. Matsuzaki, K. Noguchi, J. Nakasone, M. Sakanashi, T. Uchida, M. Kina-Tanada, H. Kubita, K. Arakaki, A. Tanimoto, N. Yanagihara, M. Sakanashi, Y. Ohya, H. Masuzaki, S. Ishiuchi, K. Sugahara, M. Tsutsui
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Journal Title
Circulation Journal
Volume: 77 (7)
Pages: 1827-1837
NAID
Related Report
Peer Reviewed
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[Journal Article] Dihydrobiopterin による内皮型一酸化窒素合成酵素機能障害2013
Author(s)
野口克彦, 濱舘直史, 松崎俊博, 坂梨まゆ子, 仲宗根淳子, 内田太郎, 新垣久美子, 久保田陽秋, 石内勝吾, 益崎裕章, 須加原一博, 大屋祐輔, 坂梨又郎, 筒井正人
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Journal Title
Ryukyu Med J
Volume: 32(1, 2)
Pages: 7-12
Related Report
Peer Reviewed
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[Journal Article] Long-Term Treatment With San’o-Shashin-To, a Kampo Medicine, Markedly Ameliorates Cardiac Ischemia-Reperfusion Injury in Ovariectomized Rats via the Redox-Dependent Mechanism2013
Author(s)
Sakanashi M, Matsuzaki T, Noguchi K, Nakasone J, Sakanashi M, Uchida T, Kina-Tanada M, Kubota H, Arakaki K, Tanimoto A, Yanagihara N, Sakanashi M, Ohya Y, Masuzaki H, Ishiuchi S, Sugahara K, Tsutsui M
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Journal Title
Circulation Journal
Volume: 77
Issue: 7
Pages: 1827-1837
DOI
NAID
ISSN
1346-9843, 1347-4820
Related Report
Peer Reviewed
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[Journal Article] Increasing dihydrobiopterin causes dysfunction of endothelial nitric oxide synthase in rats in vivo2011
Author(s)
Noguchi K, Hamadate N, Matsuzaki, T, Sakanashi M, Nakasone J, Arakaki K, Kubota H, Sugahara K, Ohya Y, Sakanashi M, Tsutsui M
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Journal Title
Am J Physiol Heart Circ Physiol
Volume: 301 (3)
Related Report
Peer Reviewed
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[Presentation] Dihydrobiopterin causes endothelial nitric oxide synthase dysfunction in rats in vivo2012
Author(s)
M Tsutsui, K Noguchi, T Matsuzaki, M Sakanashi, J Nakasone, T Uchida, M Tanada, H Kubota, K Sugahara, Y Ohya
Organizer
Annual meeting of AHA Scientific Sessions
Place of Presentation
Los Angels, California, U.S.A
Related Report
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