Solution of the molecular mechanism about proliferation / recurrence of the prostate cancer and the architecture of the comprehensive treatment strategy for he prostate cancer

• Project/Area Number: 23390379
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kanazawa University
• Principal Investigator: NAMIKI Mikio 金沢大学, 医学系, 教授 (70155985)
• Co-Investigator(Kenkyū-buntansha): MIZOKAMI Atsushi 金沢大学, 医学系, 准教授 (50248580) ; KADONO Yoshifumi 金沢大学, 附属病院, 助教 (10397218) ; MIWA Sotarou 金沢大学, 医学系, 協力研究員 (80507070) ; HIGASHI Tatsuya 東京理科大学, 薬学部薬学科, 教授 (90272963)
• Project Period (FY): 2011-11-18 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 前立腺癌 / SOD3 / SPARC / フラボノイド / 共培養 / 再燃前立腺癌 / ｱﾝﾄﾞﾛｹﾞﾝ / 微小環境 / 間質細胞 / エストロゲン / cDNA microarray

Research Abstract

We performed cDNA microarray analysis with normal prostate tissue and the prostate cancer tissue, and identified SOD3 as the gene which the expression attenuated with prostate cancer tissue.
We revealed that expression diminishment of SOD3 was associated with proliferation of prostate cancer, migration, and invasion. Also, we paid attention to extracellular matrix SPARC. Expression of SPARC attenuated with prostate cancer-derived stromal cells than normal stromal cells, and inhibited a proliferation and the migration of the cancer cells by inhibiting AKT phosphorylation. SPARC acted on cancer cells via integrin beta 1 as a receptor. Also, 2'-hydroxyflavanone which is the plant flavonoid showed antitumor effect for prostate cancer via apoptosis, and revealed that 2'-hydroxyflavanone inhibited androgen receptor activity, but did not inhibit androgen synthesis.

Research Products

• [Journal Article] Exogenous SPARC suppresses proliferation and migration of prostate cancer by interacting with integrin beta1 (2013)
  • Author(s): Shin M, Mizokami A, Kim J, Ofude M, Konaka H, Kadono Y, Kitagawa Y, Miwa S, Kumaki M, Keller ET, Namiki M
  • Journal Title: The Prostate Volume: 73(11) Pages: 1159-1170
  • DOI: 10.1002/pros.22664
  • Peer Reviewed
• [Journal Article] Repression of cell proliferation and androgen receptor activity in prostate cancer cells by 2'-hydroxyflavanone (2013)
  • Author(s): Ofude M, Mizokami A, Kumaki M, Izumi K, Konaka H, Kadono Y, Kitagawa Y, Shin M, Zhang J, Keller ET, Namiki M
  • Journal Title: Anticancer Res Volume: 33(10) Pages: 4453-4461
  • URL: http://ar.iiarjournals.org/content/33/10/4453.full.pdf+html
  • Peer Reviewed
• [Presentation] SOD3 Overexpression Inhibits Growth and Migration in PC-3 Human Prostate Cancer Cells (2014)
  • Author(s): Jungim Kim, et al
  • Organizer: 泌尿器分子細胞研
  • Place of Presentation: ホテルメトロポリタン山形(山形市)
• [Presentation] SOD3 Overexpression Inhibits Growth and Migration in PC-3 Human Prostate Cancer Cells (2014)
  • Author(s): Jungim Kim
  • Organizer: 泌尿器分子細胞研究会
  • Place of Presentation: ホテルメトロポリタン山形（山形県山形市）
• [Presentation] Exogenous SPARC suppresses proliferation and migration of prostate cancer by interacting with integrin beta1 (2013)
  • Author(s): M. Shin
  • Organizer: AACR Annual Meeting 2013
  • Place of Presentation: Washington Convention Center(USA)
• [Presentation] SPARC Suppresses Proliferation and Migration of LNCaP Human prostate cancer cell (2012)
  • Author(s): Shin M, et al
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)