| Project/Area Number |
23390384
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MIKI TSUNEHARU 京都府立医科大学, 医学(系)研究科(研究院), 教授 (10243239)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Takashi 京都府立医科大学, 医学研究科, 助教 (50601598)
NAKAMURA Terukazu 京都府立医科大学, 医学研究科, 講師 (10381964)
HONGO Fumiya 京都府立医科大学, 医学研究科, 助教 (80291798)
KAWAUCHI Akihiro 滋賀医科大学, 医学部, 教授 (90240952)
MIKAMI Kazuya 京都府立医科大学, 医学研究科, 講師 (10291585)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | 精巣腫瘍 / ZNF288 / セミノーマ / DNMT3B / HDAC2 / AR / TCam-2 / TSPY / TSPYL5 / 抗体精製 / Sin3A |
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| Research Abstract |
To test the hypothesis that reduction in AR transcriptional activity is associated with progression of testicular cancer, we analyze the function of ZNF288, novel AR co-repressor in testicular cancer cells. The result demonstrated that ZNF288 was able to form a co-repressor complex with HDAC2 and DNMT3B, and co-represses AR in TGCT model cell lines. Moreover, ZNF288 was shown to be an accelerator in in vivo testicular tumorigenesis assays in mice. In TCam-2, seminoma model cell line, up-regulation of AR transcriptional activity repressed cell growth.
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