Exploration of molecular targets for endometriosis-associated ovarian cancer with in vitro multistep carcinogenesis model
| Project/Area Number |
23390387
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kanazawa University |
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Principal Investigator |
KYO Satoru 金沢大学, 医学系, 准教授 (50272969)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Masahiro 金沢大学, 大学病院, 助教 (20313661)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | chocolate cyst / endometriosis / ovarian cancer / immortalization / carcinogenesis / 卵巣チョコレート嚢腫 / 癌化 / 不死化 / 子宮内膜症 / チョコレート嚢胞 / 内膜症 / 上皮細胞 / テロメレース |
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| Research Abstract |
To more understand the molecular mechanisms of ovarian carcinogenesis arising from ovarian endometrioma, we first established the immortalized epothelial cells from ovarian endometrioma via overexpressing cyclinD1/cdk4/hTERT cDNA. Based on the genetic analyses of clinical samples, we introduced several genetic factors into these cells to observe the transformed phenotypes. Introduction of dominant negative p53, oncogenic K-ras alleles, together with c-myc overexpression, enabled cells to obtain transformed phenotypes. These 3 steps appeared to be essential for ovarian carcinogenesis associated with endometriosis and thereby will be a promissing molecular target for the novel treatment modality.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Plasma Membrane proteomics identifies bone marrow stromal antigen 2 as a potential therapeutic target in endometrial cancer2013
Author(s)
Yokoyama T, Enomoto T, Serada S, Morimoto A, Matsuzaki S, Ueda Y, Yoshino K, Fujita M, Kyo S, Iwahori K, Fujimoto M, Kimura T and Naka T
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Journal Title
Int.J .Cancer
Volume: 132
Pages: 472-484
Related Report
Peer Reviewed
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[Journal Article] Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells2013
Author(s)
Teng Z, Yoshida T, Okabe M, Toda A, Higuchi O, Nogami M, Yoneda N, Zhou K, Kyo S, Kiyono T, Nikaido T
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Journal Title
Cell Transplant
Volume: 22
Pages: 267-278
Related Report
Peer Reviewed
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[Journal Article] Forkhead transcription factor FOXO1 is a direct target of progestin to inhibit endometrial epithelial cell growth2011
Author(s)
Kyo S, Sakaguchi J, Kiyono T, Shimizu Y, Maida Y, Mizumoto Y, Mori N, Nakamura M, Takakura M, Miyake M, Sakamoto M and Inoue M
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Journal Title
Clin.Cancer Res
Volume: 17
Pages: 525-537
Related Report
Peer Reviewed
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