Functional characterization of putative mechanoelectrical transducer channel proteins using knockout mice.
| Project/Area Number |
23390399
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Nagoya City University |
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Principal Investigator |
UGAWA SHINYA 名古屋市立大学, 医学(系)研究科(研究院), 教授 (20326135)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMURA Ken 東京医科歯科大学, その他部局等, 教授 (90010470)
NOGUCHI Yoshihiro 信州大学, 医学部, 教授 (50282752)
MURAKAMI Shingo 名古屋市立大学, 大学院医学研究科, 教授 (80157750)
UEDA Takashi 名古屋市立大学, 大学院医学研究科, 准教授 (90244540)
KAJITA Kenji 名古屋市立大学, 大学院医学研究科, 研究員 (80381820)
SAKUMA Eisuke 名古屋市立大学, 大学院医学研究科, 講師 (90295585)
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| Research Collaborator |
KROS Corne University of Sussex, UK, Dr.
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Keywords | 酸感受性イオンチャネル / メカノセンサー / 有毛細胞 / 難聴 / ノックアウトマウス / ABR / DPOAE / ASIC1b / 聴覚受容体 / 機械刺激受容チャネル / 国際研究者交流 / 英国 / ASIC4 / 内耳 / MET channel / 機械刺激電流 / パッチクランプ |
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| Outline of Final Research Achievements |
We investigated whether ASIC1b located at the ankle regions of stereocilia in mouse auditory hair cells contributes to normal auditory function, using ASIC1b knockout (KO) mice. Transmission electron microscopy demonstrated that the morphology of inner and outer hair cells of adult KO mice were normal. Patch-clamp experiments showed that immature inner and outer hair cells of wild-type mice generated large inward currents in response to acidic stimuli, and those currents were abolished in the presence of amiloride, an inhibitor of ASIC1b. Proton-induced ASIC-like currents were still detected in inner and outer hair cells of age-matched KO mice, suggesting that other ASIC subtypes also exist in mouse auditory hair cells. We are currently conducting ABR (auditory brainstem response) and DPOAE (distortion product otoacoustic emission) tests to evaluate peripheral auditory function in KO mice. Further experiments are needed to elucidate functional roles of ASIC1b in the stereocilia.
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Report
(5 results)
Research Products
(6 results)
