Identification of master transcription factors in corneal epithelial cells

• Project/Area Number: 23390404
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KINOSHITA Shigeru 京都府立医科大学, 医学(系)研究科(研究院), 教授 (30116024)
• Co-Investigator(Kenkyū-buntansha): UENO Morio 京都府立医科大学, 医学研究科, 助教 (40426531) ; KAWASAKI Satoshi 大阪大学, 医学研究科, 講師 (60347458)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 角膜上皮 / 転写因子 / ダイレクトリプログラミング / コア転写因子 / マスターレギュレーター / 角膜上皮細胞 / コア因子 / 形質転換 / エピゲノム

Research Abstract

We attempted to identify master transcription factors (TFs) in corneal epithelial cells (CECs) which are able to maintain the cell-type specific transcriptional profile. First, we used microarray analysis to select candidate master TFs in CECs from all the genes, which were highly expressed in CECs compared to various other cells. In order to narrow down the master TFs in CECs, iPS interference screening was performed and produced 20 candidate TFs. A particular combination of candidate TFs was able to induce CEC-like cells expressing keratin 12, which is a corneal specific marker when overexpressed in a heterologous cell type. Furthermore, the expression level of other corneal-related genes, such as keratin 3, keratin 15, aldehyde dehydrogenase 3, transketolase, and E-cadherin were upregulated in the induced cells. These results suggested that defined TFs, which enabled the induction of CECs, contributed to the maintenance of the corneal-specific transcriptional profile.

Research Products

• [Journal Article] Involvement of cyclin D and p27 in cell proliferation mediated by ROCK inhibitors Y-27632 and Y-39983 during corneal endothelium wound healing (2014)
  • Author(s): Okumura N, Nakano S, Kay EP, Numata R, Ota A, Sowa Y, Sakai T, Ueno M, Kinoshita S, Koizumi N
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 55(1) Pages: 318-29
  • Peer Reviewed
• [Journal Article] LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis (2014)
  • Author(s): Nakamura T, Hamuro J, Takaishi M, Simmons S, Maruyama K, Zaffalon A, Bentley AJ, Kawasaki S, Nagata-Takaoka M, Fullwood NJ, Itami S, Sano S, Ishii M, Barrandon Y, Kinoshita S
  • Journal Title: J Clin Invest Volume: 124(1) Pages: 385-397
  • Peer Reviewed
• [Journal Article] LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis (2014)
  • Author(s): Nakamura T, Hamuro J, Takaishi M, Simmons S, Maruyama K, Zaffalon A, Bentley AJ, Kawasaki S, Nagata-Takaoka M, Fullwood NJ, Itami S, Sano S, Ishii M, Barrandon Y, Kinoshita S.
  • Journal Title: J Clin Invest Volume: １ Issue: 1 Pages: 385-97
  • DOI: 10.1172/jci71488
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of cyclin D and p27 in cell proliferation mediated by ROCK inhibitors Y-27632 and Y-39983 during corneal endothelium wound healing. (2014)
  • Author(s): Okumura N, Nakano S, Kay EP, Numata R, Ota A, Sowa Y, Sakai T, Ueno M, Kinoshita S, Koizumi N.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: １ Issue: 1 Pages: 318-29
  • DOI: 10.1167/iovs.13-12225
  • Peer Reviewed
• [Journal Article] Clinical outcomes in descemet stripping automated endothelial keratoplasty with internationally shipped precut donor corneas. (2014)
  • Author(s): Nakagawa H, Inatomi T, Hieda O, Sotozono C, Yokoi N, Iliakis B, Miller TD, Ulrickson C, Kinoshita S.
  • Journal Title: Am J Ophthalmol Volume: １ Issue: 1 Pages: 50-55.e1
  • DOI: 10.1016/j.ajo.2013.09.016
  • Peer Reviewed
• [Journal Article] Cultivation of Corneal Endothelial Cells on a Pericellular Matrix Prepared from Human Decidua-Derived Mesenchymal Cells. (2014)
  • Author(s): Numata R, Okumura N, Nakahara M, Ueno M, Kinoshita S, Kanematsu D, Kanemura Y, Sasai Y, Koizumi N
  • Journal Title: Plos One Volume: 2 Issue: 2 Pages: e88169-e88169
  • DOI: 10.1371/journal.pone.0088169
  • Peer Reviewed
• [Journal Article] Investigation of gene therapy using immortalized cells derived from a Gelatioous Drop-Like Dystrophy patient (2013)
  • Author(s): Kitazawa K, Kawasaki S, Aoi K, Shinomiya K, Matsuda A, Funaki T, Nakatsukasa M, Murakami A, Kinoshita S
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 54(8) Pages: 5701-11
  • Peer Reviewed
• [Journal Article] Corneal endothelial cell fate is maintained by LGR5 via the regulation of hedgehog and wnt pathway (2013)
  • Author(s): Hirata-Tominaga K, Nakamura T, Okumura N, Kawasaki S, Kay EP, Barrandon Y, Koizumi N, Kinoshita S
  • Journal Title: Stem Cells Volume: 31(7) Pages: 1396-1407
  • Peer Reviewed
• [Journal Article] Corneal endothelial expansion promoted by human bone marrow mesenchymal stem cell-derived conditioned medium (2013)
  • Author(s): Nakahara M, Okumura N, Kay EP, Hagiya図3図4 M, Imagawa K, Hosoda Y, Kinoshita S, Koizumi N
  • Journal Title: PLoS One Volume: 8(7)
  • Peer Reviewed
• [Journal Article] 角膜の再生医療 (2013)
  • Author(s): 上野盛夫, 中村隆宏, 木下茂
  • Journal Title: 日本医師会雑誌 Volume: 142(4) Pages: 777-780
• [Journal Article] Visual improvement following cultivated oral mucosal epithelial transplantation (2013)
  • Author(s): Sotozono C, Inatomi T, Nakamura T, Koizumi N, Yokoi N, Ueta M, Matsuyama K, Miyakoda K, Kaneda H, Fukushima M, Kinoshita S
  • Journal Title: Ophthalmology Volume: 120(1) Pages: 193-200
  • Peer Reviewed
• [Journal Article] Establishment of a human corneal epithelial cell line lacking the functional TACSTD2 gene as an in vitro model for gelatinous drop-like dystrophy. (2013)
  • Author(s): Kitazawa K, Kawasaki S, Shinomiya K, Aoi K, Matsuda A, Funaki T, Yamasaki K, Nakatsukasa M, Ebihara N, Murakami A, Hamuro J, Kinoshita S.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: ８ Issue: 8 Pages: 5701-11
  • DOI: 10.1167/iovs.12-11043
  • Peer Reviewed
• [Journal Article] Classification of secondary corneal amyloidosis and involvement of lactoferrin. (2013)
  • Author(s): Kaoru Araki-sasaki, Koji Hirano, Yasuhiro Osakabe, Masahiko Kuroda, Kazuko Kitagawa, Hiroshi Mishima, Hiroto Obata, Masakazu Yamada, Naoyuki Maeda, Kohji Nishida, Shigeru Kinoshita.
  • Journal Title: Ophthalmology Volume: 120 Issue: 6 Pages: 1166-1172
  • DOI: 10.1016/j.ophtha.2012.11.047
  • Peer Reviewed
• [Journal Article] Corneal Endothelial Cell Fate is Maintained by LGR5 via the Regulation of Hedgehog and Wnt Pathway. (2013)
  • Author(s): Hirata-Tominaga K, Nakamura T, Okumura N, Kay EP, Barrandon Y, Koizumi N, Kinoshita S
  • Journal Title: Stem Cells Volume: 7 Issue: 7 Pages: 1396-1407
  • DOI: 10.1002/stem.1390
  • Peer Reviewed
• [Journal Article] Corneal endothelial expansion promoted by human bone marrow mesenchymal stem cell-derived conditioned medium. (2013)
  • Author(s): Nakahara M, Okumura N, Kay EP, Hagiya M, Imagawa K, Hosoda Y, Kinoshita S, Koizumi N.
  • Journal Title: PLOS ONE Volume: 7 Issue: 7 Pages: e69009-e69009
  • DOI: 10.1371/journal.pone.0069009
  • Peer Reviewed
• [Journal Article] Human periodontal ligament cell sheets cultured on amniotic membrane substrate (2013)
  • Author(s): Adachi K, Amemiya T, Nakamura T, Honjyo KI, Kumamoto S, Yamamoto T, Bentley AJ, Fullwood NJ, Kinoshita S, Kanamura N
  • Journal Title: Oral Dis Volume: 8 Issue: 6 Pages: 582-590
  • DOI: 10.1111/odi.12176
  • Peer Reviewed
• [Journal Article] Visual Improvement after Cultivated Oral Mucosal Epithelial Transplantatio (2013)
  • Author(s): Sotozono C, Inatomi T, Nakamura T, Koizumi N, Yokoi N, Ueta M, Matsuyama K, Miyakoda K, Kaneda H, Fukushima M, Kinoshita S
  • Journal Title: Ophthalmol, Peer-Reviewed papers Volume: 120(1) Issue: 1 Pages: 193-200
  • DOI: 10.1016/j.ophtha.2012.07.053
  • Peer Reviewed
• [Journal Article] Clinical outcomes of phototherapeutic keratectomy in eyes with Thiel-Behnke corneal dystrophy. (2013)
  • Author(s): Hieda O
  • Journal Title: American Journal of Ophthalmology Volume: 155 Issue: 1 Pages: 66-72
  • DOI: 10.1016/j.ajo.2012.06.022
  • Peer Reviewed
• [Journal Article] A novel mutation (p.Glu1389AspfsX16) of the phosphoinositide kinase, FYVE finger containing gene found in a Japanese patient with fleck corneal dystrophy (2012)
  • Author(s): Kawasaki S, Yamasaki K, Nakagawa H, Shinomiya K, Nakatsukasa M, Nakai Y, Kinoshita S
  • Journal Title: Mol Vis Volume: 18 Pages: 2954-60
  • Peer Reviewed
• [Journal Article] ROCK inhibitor converts corneal endothelial cells into a phenotype capable of regenerating in vivo endothelial tissue (2012)
  • Author(s): Okumura N, Koizumi N, Ueno M, Sakamoto Y, Takahashi H, Tsuchiya H, Hamuro J, Kinoshita S
  • Journal Title: Am J Pathol Volume: 181(7) Pages: 268-277
  • Peer Reviewed
• [Journal Article] A selective inhibitor of the Rho kinase pathway, Y-27632, and its influence on wound healing in the corneal stroma (2012)
  • Author(s): Yamamoto M, Quantock AJ, Young RD, Okumura N, Ueno M, Sakamoto Y, Kinoshita S, Koizumi N
  • Journal Title: Mol Vis Volume: 18 Pages: 1727-1739
  • Peer Reviewed
• [Journal Article] Functional role of PPARδ in corneal epithelial wound healing (2012)
  • Author(s): Nakamura Y, Nakamura T, Tarui T, Inoue J, Kinoshita S
  • Journal Title: Am J Pathol Volume: 180(2) Pages: 583-598
  • Peer Reviewed
• [Journal Article] A novel mutation (p.Glu1389AspfsX16) of the phosphoinositide kinase, FYVE finger containing gene found in a Japanese patient with fleck corneal dystrophy. (2012)
  • Author(s): Kawasaki S
  • Journal Title: Molecular Vision Volume: 18 Pages: 2954-2960
  • Peer Reviewed
• [Journal Article] Atypical continuous keratitis in a case of rheumatoid arthritis accompanying with severe scleritis (2012)
  • Author(s): Hata M
  • Journal Title: Cornea Volume: 31 Issue: 12 Pages: 1493-1496
  • DOI: 10.1097/ico.0b013e31826218c7
  • Peer Reviewed
• [Journal Article] A selective inhibitor of the Rho kinase pathway, Y-27632, and its influence on wound healing in the corneal stroma (2012)
  • Author(s): Yamamoto M
  • Journal Title: Molecular Vision Volume: 18 Pages: 1727-1739
  • Peer Reviewed
• [Journal Article] Enhancement of corneal endothelium wound healing by a ROCK inhibitor eye drop (2011)
  • Author(s): Okumura N, Koizumi N, Ueno M, Sakamoto Y, Takahashi H, Hirata K, Torii R, Hamuro J, Kinoshita S
  • Journal Title: Br J Ophthalmol Volume: 95(7) Pages: 1006-1009
  • Peer Reviewed
• [Journal Article] Long-term results of autologous cultivated oralmucosal epithelial transplantation in the scar phase of severe ocular surface disorders (2011)
  • Author(s): Nakamura T, Takeda K, Inatomi T, Sotozono C, Kinoshita S
  • Journal Title: Br J Ophthalmol Volume: 95(7) Pages: 942-946
  • Peer Reviewed
• [Journal Article] Two novel mutations of TACSTD2 found in three Japanese gelatinous drop-like corneal dystrophy families with their aberrant subcellular localization (2011)
  • Author(s): Nakatsukasa M, Kawasaki S, Yamasaki K, Fukuoka H, Matsuda A, Nishida K, Kinoshita S
  • Journal Title: Molecular Vision Volume: 17 Pages: 965-970
  • Peer Reviewed
• [Presentation] Investigation of Gene Therapy and Pathophysiology Using Immortalized Cells Derived from a Gelatinous Drop-Like Dystrophy Patient (2014)
  • Author(s): Kitazawa K, Kawasaki S, Aoi K, Shinomiya K, Matsuda A, Funaki T, Nakatsukasa M, Murakami A, Kinoshita S
  • Organizer: Gordon Research Seminar
  • Place of Presentation: Ventura, USA
  • Year and Date: 2014-02-16
• [Presentation] LRIG1 による角膜の恒常性維持機構の解析 (2014)
  • Author(s): 中村隆宏, 羽室淳爾, 高石樹朗, 丸山和一, 永田真帆, 川崎論, 板見智, 佐野栄紀, 石井優, 木下茂
  • Organizer: 角膜カンファランス2014
  • Place of Presentation: 沖縄
  • Year and Date: 2014-01-31
• [Presentation] 角膜上皮細胞におけるR-spondin1 の機能解析 (2014)
  • Author(s): 大倉翔貴, 中村隆宏, 畑友衣子, 小林正和, 永田真帆, 小泉範子, 木下茂
  • Organizer: 角膜カンファランス2014
  • Place of Presentation: 沖縄
  • Year and Date: 2014-01-31
• [Presentation] 眼表面扁平上皮腫瘍におけるLRIG1発現解析 (2014)
  • Author(s): 永田真帆, 中村隆宏, 外園千恵, 稲富勉, 横井則彦, 木下茂
  • Organizer: 角膜カンファランス2014
  • Place of Presentation: 沖縄
  • Year and Date: 2014-01-31
• [Presentation] ヒト角膜上皮細胞シートの安定的な製造を可能にするシート作製技術の開発 (2014)
  • Author(s): 前田謙一, 横山修一, 細田勇喜, 渡部俊介, 今川究, 中村隆宏, 木下茂
  • Organizer: 角膜カンファランス2014
  • Place of Presentation: 沖縄
  • Year and Date: 2014-01-30
• [Presentation] ヒト角膜上皮細胞シートの安定的な製造を可能にするシート作製技術の開発 (2014)
  • Author(s): 135. 前田謙一, 横山修一, 細田勇喜, 渡部俊介, 今井究, 中村隆宏, 木下茂
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
• [Presentation] LRIG1による角膜の恒常性維持機構の解析 (2014)
  • Author(s): 中村隆宏, 羽室淳爾, 高石樹朗, 丸山和一, 永田真帆, 川崎論, 板見智, 佐野栄紀, 石井優, 木下茂
  • Organizer: 角膜カンファランス2014
  • Place of Presentation: 沖縄
• [Presentation] 角膜上皮細胞におけるR-spondin1の機能解析 (2014)
  • Author(s): 大倉翔貴, 中村隆宏, 畑友衣子, 小林正和, 永田真帆, 小泉範子, 木下茂
  • Organizer: 角膜カンファランス2014
  • Place of Presentation: 沖縄
• [Presentation] シリコンオイル注入眼に併発した帯状角膜変性に対しPTKを施行した2例 (2014)
  • Author(s): 宇山紘史, 中村隆宏, 石田和寛, 外園千恵, 稲富勉, 木下茂, 栗本康夫
  • Organizer: 角膜カンファランス2014
  • Place of Presentation: 沖縄
• [Presentation] 先進医療としての羊膜移植の現状と有効性 (2014)
  • Author(s): 森川恵輔, 外園千恵, 稲富勉, 中村隆宏, 横井則彦, 松尾俊康, 木下茂
  • Organizer: 角膜カンファランス2014
  • Place of Presentation: 沖縄
• [Presentation] Investigation of gene therapy using immortalized cells derived from a Gelatioous Drop-Like Dystrophy patient (2013)
  • Author(s): Kitazawa K, Kawasaki S, Aoi K, Shinomiya K, Matsuda A, Funaki T, Nakatsukasa M, Murakami A, Kinoshita S
  • Organizer: The Association for Research in Vision and Ophthalmology, Seatle (ARVO) 2013
  • Place of Presentation: Seatle, USA
  • Year and Date: 2013-05-08
• [Presentation] Integral Analysis of Gene Signatures and MicroRNA Expression of Cultured Human Corneal Endothelial Cells in Relation to Their Functions, Cell Senescence, Epithelial-Mesenchymal Transition, and Fibrosis (2013)
  • Author(s): Asada K, Toda M, Hagiya M, Nakata K, Ueno M, Okumura N, Koizumi N, Hamuro J, Kinoshita S
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-05-07
• [Presentation] Transfer of TACSTD2 gene into corneal epithelial cells of Gelatinous drop like corneal dystrophy and its functional expression (2013)
  • Author(s): Matsunaga T, Kitazawa K, Yamasaki K, Sato T, Watanabe Y, Funaki T, Matsuda A, Ebihara N, Kawasaki S, Murakami A
  • Organizer: The Association for Research in Vision and Ophthalmology, Seatle (ARVO) 2013
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-05-07
• [Presentation] Efficacy and Safety Evaluation of Cell-Injection Therapy using Cultivated Human Corneal Endothelial Cells (2013)
  • Author(s): Koizumi N, Okumura N, Shiina T, Suzuki S, Nakamura S, Sakamoto Y, Yamasaki K, Ueno M, Hamuro J, Kinoshita S
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-05-06
• [Presentation] Rho kinase inhibitor promotes cell adhesion of corneal endothelial cells through inhibiting phosphorylation of MLC (2013)
  • Author(s): Tsujimoto Y, Okumura N, Kay EP, Numata R, Kinoshita S, Koizumi N
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-05-06
• [Presentation] A new method for the efficient and less-stress dissociation of corneal epithelial cells (2013)
  • Author(s): Shinomiya K, Kawasahi S, Aoi K, Kitazawa K, Kinoshita S
  • Organizer: The Association for Research in Vision and Ophthalmology, Seatle (ARVO) 2013
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-05-05
• [Presentation] The Biological Characteristics and Clinical Application of Human (2013)
  • Author(s): Shigeru Kinoshita
  • Organizer: Kyoto International Workshop for Visual Science 2013
  • Place of Presentation: Kyoto
• [Presentation] Investigation of Gene Therapy Using Immortalized Cells Derived from a Gelatinous Drop-Like Corneal Dystrophy Patient (2013)
  • Author(s): K Kitazawa, S Kawasaki, K Aoi, K Shinomiya, A Matsuda, T Funaki, M Nakatsukasa, J Hamuro, S Kinoshita
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle
• [Presentation] Cell Line of Fuchs' Corneal Dystrophy Produces an Abnormal Extracellular Matrix. (2013)
  • Author(s): Leona Ho, Naoki Okumura, EunDuck P. Kay, Kenta Yamasaki, Satoshi Kawasaki, Theofilos Tourtas, Ursula Schlotzer-Schrehardt, Friedrich E. Kruse, Shigeru Kinoshita, Noriko Koizumi
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle
• [Presentation] Rho-kinase inhibitor enhances corneal endothelial cell proliferation via p27 degradation. (2013)
  • Author(s): Ryohei Numata, Naoki Okumura, EunDuck P. Kay, Makiko Nakahara, Shinichiro Nakano, Morio Ueno, Shigeru Kinoshita, Noriko Koizumi
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle
• [Presentation] Proliferation Propensity of Cultured Human Corneal Endothelial Cells and Their Plasticity Dictated by Culture Microenvironments. (2013)
  • Author(s): Munetoyo Toda, Kana Nakata, kazuko asada, Michio Hagiya, Morio Ueno, Naoki Okumura, Noriko Koizumi, Junji Hamuro, Shigeru Kinoshita:
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle
• [Presentation] Cell-injection Therapy Using Rho Kinase Inhibitor in a Corneal Endothelial Dysfunction Rabbit Model (2013)
  • Author(s): Junji Kitano, Naoki Okumura, EunDuck P. Kay, Morio Ueno, Junji Hamuro, Shigeru Kinoshita, Noriko Koizumi
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle
• [Presentation] Rho kinase inhibitor promotes cell adhesion of corneal endothelial cells through inhibiting phosphorylation of MLC. (2013)
  • Author(s): Yuki Tsujimoto, Naoki Okumura, EunDuck P. Kay, Ryohei Numata, Shigeru Kinoshita, Noriko Koizumi
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle
• [Presentation] Efficacy and Safety Evaluation of Cell-Injection Therapy using Cultivated Human Corneal Endothelial Cells. (2013)
  • Author(s): Noriko Koizumi, Naoki Okumura, Takashi Shiina, Shingo Suzuki, Shinichiro Nakamura, Yuji Sakamoto, Kenta Yamasaki, Morio Ueno, Junji Hamuro, Shigeru Kinoshita
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle
• [Presentation] Development of a Human Cultivated Nasal Mucosal Epithelial Cell Sheet for the Treatment of Severe Ocular Surface Diseases. (2013)
  • Author(s): Masakazu Kobayashi, Takahiro Nakamura, Makoto Yasuda, Yuiko Hata, Shoki Okura, Naoki Okumura, Noriko Koizumi, Hisao Yasu, Shigeru Kinoshita
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle
• [Presentation] Integral Analysis of Gene Signatures and MicroRNA Expression of Cultured Human Corneal Endothelial Cells in Relation to Their Functions, Cell Senescence, Epithelial-Mesenchymal Transition, and Fibrosis. (2013)
  • Author(s): Kazuko Asada, Munetoyo Toda, Michio Hagiya, Kana Nakata, Morio Ueno, Naoki Okumura, Noriko Koizumi, Junji Hamuro, Shigeru Kinoshita
  • Organizer: The Association for Research in Vision and Ophthalmology 2013
  • Place of Presentation: Seattle
• [Presentation] 細胞障害が少ない角膜上皮細胞分離法の検討 (2013)
  • Author(s): 篠宮克彦
  • Organizer: 第37回日本角膜学会総会
  • Place of Presentation: 和歌山
• [Presentation] 羊膜を基質とし作成した口腔粘膜再生シートを用いた尿路再建 (2012)
  • Author(s): 内藤泰行, 河内明宏, 邵仁晢, 中村隆宏, 稲富勉, 木下茂, 美紀恒治
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-12
• [Presentation] Establishment of immortalized corneal and conjunctival epithelial cells lacking the functional TACSTD2 gene (2012)
  • Author(s): Kitazawa K, Kawasaki S, Shinomiya K, Matsuda A, Funaki F, Yamasaki K, Nakatsukasa M, Fukuoka H, Ebihara N, Murakami A, Kinoshita S
  • Organizer: The Association for Research in Vision and Ophthalmology, Fort Lauderdale (ARVO) 2012
  • Place of Presentation: Ft. Lauderdale, USA
  • Year and Date: 2012-05-07
• [Presentation] 結膜腫瘍でのLeucine-rich repeats and immunoglobulin-like domains 1(LRIG1)の発現 (2012)
  • Author(s): 永田真帆, 中村隆宏, 外園千恵, 稲富勉, 横井則彦, 板見智, 木下茂
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-05
• [Presentation] 角膜上皮化生をきたした培養口腔粘膜上皮移植の1 例 (2012)
  • Author(s): 張佑子, 外園千恵, 中川絋子, 中村隆宏, 稲富勉, 上田真由美, 横井則彦, 木下茂
  • Organizer: 角膜カンファランス2012
  • Place of Presentation: 東京
  • Year and Date: 2012-02-23
• [Presentation] 角膜扁平上皮癌におけるGPR49 の発現の検討 (2012)
  • Author(s): 平田香菜, 中村隆宏, 外園千恵, 稲富勉, 奥村直毅, 小泉範子, 横井則彦, 木下茂
  • Organizer: 角膜カンファランス2012
  • Place of Presentation: 東京
  • Year and Date: 2012-02-23
• [Presentation] 全層角膜移植の11 年間の動向と治療成績 (2012)
  • Author(s): 堀内稔子, 中川絋子, 稲富勉, 上田真由美, 中村隆宏, 小泉範子, 外園千恵, 横井則彦, 木下茂
  • Organizer: 角膜カンファランス2012
  • Place of Presentation: 東京
  • Year and Date: 2012-02-23
• [Presentation] 粘膜類天疱瘡の発症背景の関する検討 (2012)
  • Author(s): 福本暁子
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] 膠様滴状角膜ジストロフィ患者由来の不死化角膜および結膜上皮細胞の樹立 (2012)
  • Author(s): 北澤耕司
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] Cultivated Oral Mucosal Epithelial Transplantation for Ocular Surface Reconstruction in Stevens-Johnson Syndrome (2011)
  • Author(s): Sotozono C, Inatomi T, Nakamura T, Ueta M, Yokoi N, Miyakoda K, Kinoshita S
  • Organizer: 第115回米国眼科学会議(AAO)
  • Place of Presentation: Orlando, USA
  • Year and Date: 2011-10-24
• [Presentation] 難治性角結膜疾患に対する自家培養口腔粘膜上皮シート移植のレトロスペクティブ調査 (2011)
  • Author(s): 外園千恵, 稲富勉, 中村隆宏, 小泉範子, 横井則彦, 都田桂子 , 松山琴音, 木下茂
  • Organizer: 第65回日本臨床眼科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-08
• [Presentation] 自家培養口腔粘膜上皮シート移植による結膜嚢再建効果の検討 (2011)
  • Author(s): 稲富勉, 外園千恵, 中村隆宏, 小泉範子, 都田桂子, 松山琴音, 木下茂
  • Organizer: 第65回日本臨床眼科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-08
• [Presentation] Epigenetic Regulation and Identification of the Cis-Regulatory Region of the Keratin 12 Gene in Rabbit Cornea (2011)
  • Author(s): Satoshi Kawasaki, Kenta Yamasaki, Katsuhiko Shinomiya, Shigeru Kinoshita
  • Organizer: ARVO (Association of Research in Vision and Ophthalmology)
  • Place of Presentation: Florida, USA
  • Year and Date: 2011-05-01
• [Book] Corneal Dystropies (2011)
  • Author(s): Kawasaki S, Kinoshita S
  • Total Pages: 159
  • Publisher: Karger