Visualization of functional dynamics in bone cells by three dimensional fluorescence imaging toward understanding of bone physiopathology
Project/Area Number |
23390417
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Morphological basic dentistry
|
Research Institution | Ehime University (2013) Tokyo Medical and Dental University (2011-2012) |
Principal Investigator |
IIMURA Tadahiro 愛媛大学, プロテオサイエンスセンター, 准教授(特定教員) (20282775)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
|
Keywords | イメージング / 骨代謝 / 骨細胞 / 骨 / 骨格発生 / 蛍光イメージング / 形態計測 |
Research Abstract |
The purpose of this study is to unveil novel mechanisms of bone pathophysiology by exploiting three-dimensional (3D) fluorescence morphometry. After acquisition of fluorescence optical sections, 3D images were reconstructed in silico, by which. detailed distribution pattern and signal intensity of molecule of interest can be quantitatively analyzed. We successfully measured and compared osteocyte-network pattern in bones loaded by distinct physiological tensions. Spatio-temporal dynamics of Sclerostion production by osteocytes during postnatal bone development was described. Furthermore, we uncovered unique regulatory mechanism of FGF23 by DMP1.
|
Report
(4 results)
Research Products
(43 results)
-
-
-
-
-
-
-
-
[Journal Article] Spatiotemporal disorder in endochondral ossification during axial skeleton development in the Mesp2-null mouse: A developmental etiology of spondylocostal dysostosis and spondylothoracic dysostosis.2013
Author(s)
Makino Y, Takahashi Y, Tanabe R, Tamamura Y, Watanabe T, Haraikawa M, Hamagaki M, Hata K, Kanno J, Yoneda T, Saga Y, Goseki-Sone M, Kaneko K, Yamaguchi A, Iimura T
-
Journal Title
BONE
Volume: 53
Issue: 1
Pages: 248-258
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
[Journal Article] Retention of bone strength by feeding of milk and dairy products in ovariectomized rats; involvement of changes in serum levels of 1alpha, 25(OH)2D3 and FGF23.2012
Author(s)
Tanabe R, Haraikawa M, Sogabe N, Sugimoto A, Kawamura Y, Takasugi S, Nagata M, Nakane A, Yamaguchi A, Iimura T, Masae Goseki-Sone
-
Journal Title
J Nutr Biochem
Volume: in press
Issue: 6
Pages: 1000-7
DOI
Related Report
Peer Reviewed
-
[Journal Article] CxCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction2012
Author(s)
Oue E, Lee Ji-Won, Sakamoto K, Iimura K, Aoki K, Kayamori K, Michi Y, Yamagshiro M, Harada K, Amagasa T, Yamaguchi A
-
Journal Title
Biochem Biophys Res Commun
Volume: 424
Issue: 3
Pages: 456-461
DOI
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Cdc42 is required for chondrogenesis and interdigital programmed cell death during limb development2011
Author(s)
Aizawa R, Yamada A, Suzuki D, Iimura T, Kassai H, Harada T, Tsukasaki M, Yamamoto G, Tachikawa T, Nakao K, Yamamoto M, Yamagchi A, Aiba A, Kamijo R
-
Journal Title
AMech Dev
Volume: (In press)
Issue: 1-4
Pages: 38-50
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-