| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Research Abstract |
The purpose of this study is to unveil novel mechanisms of bone pathophysiology by exploiting three-dimensional (3D) fluorescence morphometry. After acquisition of fluorescence optical sections, 3D images were reconstructed in silico, by which. detailed distribution pattern and signal intensity of molecule of interest can be quantitatively analyzed. We successfully measured and compared osteocyte-network pattern in bones loaded by distinct physiological tensions. Spatio-temporal dynamics of Sclerostion production by osteocytes during postnatal bone development was described. Furthermore, we uncovered unique regulatory mechanism of FGF23 by DMP1.
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