| Project/Area Number |
23390424
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
JIMI EIJIRO 九州歯科大学, 歯学部, 教授 (40276598)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Kazuhiro 東京医科歯科大学, 歯学部, 准教授 (40272603)
KATAGIRI Takenobu 埼玉医科大学, 医学部, 教授 (80245802)
FUKUSHIMA Hidefumi 九州歯科大学, 九州歯科大学, 福岡歯科大学, 歯学部, 歯学部, 歯学部, 助教, 講師, 准教授 (70412624)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | 骨芽細胞 / 破骨細胞 / NF-κB / NF-κBの非古典的経路 / 骨代謝調節 / 破骨細胞分化 / 骨芽細胞分化 / BMP |
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| Research Abstract |
We investigated the molecular mechanisms triggered by the alternative NF-kB pathway in the regulation of osteoblast and osteoclast differentiation. Osteoblastic differentiation induced by BMP2, as shown by alkaline phosphatase activity. Smad1 phosphorylation was also enhanced in primary osteoblasts from aly/aly mice. The ectopic bone formation in vivo that was induced by BMP2 was enhanced in aly/aly mice compared with controls. On the other hand, the overexpression of RelB in aly/aly bone marrow cells rescues RANKL-induced osteoclastogenesis by inducing p100 processing. Cot, an MAP3K, was up-regulated by RelB overexpression and knocking down of Cot expression significantly reduced the RANKL-induced osteoclastogenesis induced by RelB overexpression. Thus, the alternative the NF-kB pathway via the processing of p52 from p100 negatively regulates osteoblastic differentiation by modifying BMP activity and positively regulates RANKL-induced osteoclastogenesis.
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