| Project/Area Number |
23390425
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Takao 北海道医療大学, 歯学部薬理学分野, 講師 (20326549)
NEZU Akihiro 北海道医療大学, 歯学部薬理学分野, 講師 (00305913)
東城 庸介 北海道医療大学, 歯学部, 教授 (90111731)
和泉 博之 北海道医療大学, 歯学部, 教授 (20108541)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
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| Keywords | 唾液腺 / イメージング / カルシウム / in vivo / イノシトール三リン酸 / 血流 / 遺伝子導入 / ウイルスベクター / カルシウムシグナル / 唾液分泌 / 発光センサー / 蛍光イメージング / 口腔乾燥症 / サイクリックAMP |
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| Outline of Final Research Achievements |
We succeeded in the in vivo imaging of Ca2+ responses in salivary gland in live rats. This novel method allowed to demonstrate the tissue-wide synchronization of Ca2+ oscillations in acetylcholine-stimulated submandibular glands, and parasympathetic nerves mediated Ca2+ responses. In addition, new techniques for the real time monitoring of salivary secretions and blood flow imaging clarified the regulatory mechanisms for salivary secretions. We also developed a method, CFLA, for the measurement of IP3 using fluorescent ligands and ligand binding domain of IP3 receptors. In comparison with the LIBRA, a FRET based IP3 sensor, the new methods provided 5-times larger FRET signal. This method was also applied for quantitative analysis of IP3.
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