| Project/Area Number |
23390427
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MIURA Masahiko 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (10272600)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Akira 東京医科歯科大学, 医歯学総合研究科, 教授 (00142430)
HAYASHI Yoshio 東京薬科大学, 薬学部 (10322562)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | 分子イメージング / 放射線治療 / 腫瘍微小環境 |
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| Research Abstract |
Cell kinetics is thought to be an important factor that significantly affects cellular radiosensitivity in solid tumors; however, it was almost impossible to technically detect its dynamics in a condition that cells are alive. It is highly speculated that cells should exhibit a different cell kinetics in tumor microenvironments from that in monolayer cultures. In this study, we examined how tumor cells show the change of cell kinetics in tumor microenvironments following irradiation, using a novel technique to visualize the cell cycle. We succeeded in visualizing the difference in radiation-induced cell kinetics between outer- and inner-layers in multicellular spheroids. Furthermore, we found that radiation-induced G2 arrest was remarkably prolonged in tumor microenvironments. Considering that cells in the cell cycle phase of G2 arrest is radiosensitive, the present findings should provide important clinical implications in a fractionated regimen.
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