| Project/Area Number |
23390437
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
ISHII Nobuyuki 神奈川歯科大学, 歯学研究科(研究院), 教授 (20163610)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGAKI Yuko 神奈川歯科大学, 大学院・歯学研究科(研究院), 教授 (60050689)
WATANABE Kiyoko 神奈川歯科大学, 大学院・歯学研究科(研究院), 講師 (70148021)
MUTOH Noriko 神奈川歯科大学, 大学院・歯学研究科(研究院), 講師 (40510433)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Keywords | 歯学 / 再生医学 / セメント芽細胞 / MTA / 根管充填 / セメント質 |
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| Research Abstract |
The ideal healing of an endodontic treatment is a mineralized tissue formation on apical foramen. This study aimed at developing the new endodontic treatment which can heal by using Mineral Trioxide Aggregate(MTA) and S-PRG filler by controlling hard tissue formation of the cell which present in the periapical tissue. We used the cells that a human cementblast (HCEM), a human pulp chamber cell (HPulp) and a human periodontal ligament cell (HPLC). The co-culture with MTA and cement containing S-PRG filler. We investigated the expression of genes involved in mineralized tissue formation (Bone Sialoprotein ; BSP, Osteocalcin ; OCN), and mineralization (Alkaline phosphatase ; ALP).It was shown that BSP, OCN and ALP revelation become the maximum in 24 hours and as for all the cells, the potential of mineralized tissue ( bone or cementum) was observed. MTA and S-PRG filler promoting the differentiation induction of HCEM, HPulp, and HPLC participating in the mineralized tissue formation.
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